1. Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①

2. 2 | Biochemistry laboratory 1. Aims of Biochemistry Experiments  Experiments Rules  Instruments Introduction

3. GRADING Theory EXAM : 70% Attendance(Theory): 10% Lab Performance: 20%

4. 4 | 2. Experiment Rules ① Put on your white lab coat before the class begin Biochemistry laboratory

5. 5 | 2. Experiment Rules ② It is better done than said Biochemistry laboratory

6. 6 | 2. Experiment Rules ③ Make Compensation For Any Damage Biochemistry laboratory

7. 7 | 2. Experiment Rules ④ Please shut off the faucet and Power Biochemistry laboratory

8. 8 | 2. Experiment Rules ⑤ Clean the classroom before you leave! Biochemistry laboratory

9. If you break any rules above, I will lower your participation grade !

10. 10 | 3. Instruments Introduction ① check instruments for each group by the table below categorynumbercategorynumber 250-ml beaker1Dropper1 100-ml beaker1Glass stirring rod1 Large Test tube18Test-tube rack1 Small Test tube6Bulb for pipette1 Biochemistry laboratory

11. 11 | 3. Instruments Introduction Beaker Biochemistry laboratory

12. 12 | 3. Instruments Introduction Test tube Biochemistry laboratory

13. Solution Mixing  Rotation  Fliping  Shaking Biochemistry laboratory

14. 3. Instruments Introduction Test-tube rack Biochemistry laboratory

15. 15 | 3. Instruments Introduction T est-tube brush Biochemistry laboratory

16. Test tube Washing 1. Flush the test tube with water At least 3 times 2. Scrub it with detergent both inside & outside 3.Flush the test tube with water at least 5 times just like step1 4.Rinse it with distilled water and place them upside down on the rack to leave it dry Biochemistry laboratory

17. 17 | 3. Instruments Introduction Dropper Biochemistry laboratory

18. 3. Instruments Introduction Glass-stirring rod Biochemistry laboratory

19. 19 | 3. Instruments Introduction Tube clip Biochemistry laboratory

20. 3. Instruments Introduction Bulb for pipette Biochemistry laboratory

21. 3. Instruments Introduction Scaled pipette Biochemistry laboratory

22. Scaled Pipette Pattern: 0.5 ml 、 1 ml 、 2 ml 5 ml 、 10 ml etc. Biochemistry laboratory

23. Hold Like This Biochemistry laboratory

24. Usage Hold pipette Absorb Solution Adjust the Volume Drop Solution Biochemistry laboratory

25. 3. Instruments Introduction Micropipette Biochemistry laboratory

26. Micropipette Specifications: 10 μl 、 20 μl 、 100 μl 、 200 μl 、 1000 μl & 5000 μl Biochemistry laboratory

27. THE INTRODUCTION ON PIPETTE Biochemistry laboratory

28. THE INTRODUCTION ON PIPETTE Biochemistry laboratory

29. Usage 1.Set the volume of solution 2.Choose paired tip,and put on itpaired 3.Absorb solution with 1 st Lock, drop solution with 2 nd Lock.

30. Summary 1. Be careful 2. Be patient 3. Be lively Biochemistry laboratory

31. Please hand in your Report in time ! Thank you Biochemistry laboratory Changsha Medical University

32. LAB REPORT