1. Material Safety Data Sheets Interpreting and Understanding Information on a MSDS

2. Toxic effects of Laboratory Chemicals In order to minimize the hazards associated with chemicals used in the laboratory the researcher must investigate many sources of information to safely design an experiment. There are many ways to do this. The starting point should be with a review of a MSDS. In order to minimize the hazards associated with chemicals used in the laboratory the researcher must investigate many sources of information to safely design an experiment. There are many ways to do this. The starting point should be with a review of a MSDS.

3. Hazard Communication Hazard Communication Standard Hazard Communication Standard 29CFR 1910.1200 29CFR 1910.1200 OSHA published in 1988, requires, chemical manufacturers or importers to evaluate the hazards of the products they supply and summarize this information on Material Safety Data Sheets (MSDS), shipping labels, and product warnings OSHA published in 1988, requires, chemical manufacturers or importers to evaluate the hazards of the products they supply and summarize this information on Material Safety Data Sheets (MSDS), shipping labels, and product warnings

4. Material Safety Data Sheets (MSDS) Required to provide lab workers with proper procedures for handling substances safely Required to provide lab workers with proper procedures for handling substances safely Each MSDS is unique to a specific compound and manufacturer Each MSDS is unique to a specific compound and manufacturer When reagents are ordered, and MSDS is shipped with them When reagents are ordered, and MSDS is shipped with them MSDS also available online or on discs MSDS also available online or on discs

5. What is on a MSDS? Company and chemical ID Company and chemical ID Name, address and telephone number of manufacturer Name, address and telephone number of manufacturer Formula (composition) Formula (composition) Health Hazards Health Hazards Primary routes of entry into body Primary routes of entry into body Any general precautions for safe handling and use of the chemical Any general precautions for safe handling and use of the chemical Physical and chemical properties Physical and chemical properties Emergency and First aid procedures Emergency and First aid procedures Stability and reactivity Stability and reactivity Toxicology information Toxicology information Preventative measures Preventative measures Waste disposal Waste disposal Storage Storage Spills or leaks Spills or leaks MSDS date of preparation MSDS date of preparation

7. Routes of Exposure Inhalation - Most common route of exposure, lungs are designed for maximum transport and adsorption of vapors, large surface area (1000 sf) Inhalation - Most common route of exposure, lungs are designed for maximum transport and adsorption of vapors, large surface area (1000 sf) Dermal – Second most common route of exposure, lipid (pass with greater ease) and water soluble chemicals can pass through the skin. Has 20 sf surface area. Dermal – Second most common route of exposure, lipid (pass with greater ease) and water soluble chemicals can pass through the skin. Has 20 sf surface area. Ingestion – can occur through food contamination, eating drinking in lab, poor hygiene, mucociliary transport of vapors trapped in upper air ways, Ingestion – can occur through food contamination, eating drinking in lab, poor hygiene, mucociliary transport of vapors trapped in upper air ways, Injection – Can occur through injury and needle sticks Injection – Can occur through injury and needle sticks

8. Lethal Dose An LD 50 value is the amount of a solid or liquid material that it takes to kill 50% of test animals in one dose. The dose may be administered orally (by mouth), or injection into various parts of the body. The value is usually reported along with the administration method. An LD 50 value is the amount of a solid or liquid material that it takes to kill 50% of test animals in one dose. The dose may be administered orally (by mouth), or injection into various parts of the body. The value is usually reported along with the administration method.

9. Acute Toxicity Levels Toxicity LD- 50 (rats) LD- 50(rabbits) LC- 50 (rats) Probable lethal human dose Extremely Toxic <1mg<10ppm A taste, 1 grain Highly Toxic 1-50 mg10-100 ppm 1 tea, 4 cc Moderately Toxic 50-500 mg100-1000 ppm 1 oz, 30g. Slightly Toxic 500-5000 mg 1000- 10,000 1 pint, 250g

11. MSDS Labels

12. Red=Flammability 73 F (22 C) and a boiling point below 100 F. 73 F (22 C) and a boiling point below 100 F. 4-Materials with a flashpoint below 73 F and a boiling point below 100 F 3-Materials with a flashpoint below 73 F and a boiling point greater than or equal to 100 F (38 C) or a flashpoint above 73 F and less than 100 F. 2-Materials with a flashpoint above 100 F, but not exceeding 200 F (93.3 C). 1-Materials with a flashpoint above 200 F. 0-Materials which normally won't burn. 4-Materials with a flashpoint below 73 F and a boiling point below 100 F 3-Materials with a flashpoint below 73 F and a boiling point greater than or equal to 100 F (38 C) or a flashpoint above 73 F and less than 100 F. 2-Materials with a flashpoint above 100 F, but not exceeding 200 F (93.3 C). 1-Materials with a flashpoint above 200 F. 0-Materials which normally won't burn.

13. Blue-Health Hazard 4-Materials with an oral LD50 of less than or equal to 5 mg/kg. 3-Materials with an oral LD50 above 5, but less than 50 mg/kg. 2-Materials with an oral LD50 above 50, but less than 500 mg/kg. 1-Materials with an oral LD50 above 500, but less than 2000mg/kg. 0-Materials with an oral LD50 above 2000mg/kg.

14. Yellow=Reactivity Hazard 4-Material is capable of explosion or detonation at normal temperature and pressure. 3-Material is capable of explosion, but requires a strong initiating source, or the material reacts with water. 2-Material undergoes violent chemical changes at elevated temperature and pressure. 1-Normally stable, but can become unstable at elevated temperatures. 0-Normally stable. 4-Material is capable of explosion or detonation at normal temperature and pressure. 3-Material is capable of explosion, but requires a strong initiating source, or the material reacts with water. 2-Material undergoes violent chemical changes at elevated temperature and pressure. 1-Normally stable, but can become unstable at elevated temperatures. 0-Normally stable.

15. White = Special Hazard W Water Reactive W Water Reactive Ox Oxidizer Ox Oxidizer COR Corrosive COR Corrosive Radiation Radiation