1. CMIC National Mining Innovation Forum October 30, 2014 The Field Ready Electrochemical Detector Using Synthetic Biology to Design Contaminant Sensors

2. What is Synthetic Biology? By Definition Synthetic biology is the engineering of biology: the synthesis of complex, biologically based (or inspired) systems, which display functions that do not exist in nature. Serrano et al. 2007

3. What is Synthetic Biology? Student.societyforscience.org www2.fin.ucar.edu $0.28 Per Base Pair Plus Shipping

4. What is Synthetic Biology? “Synthetic Biology Market is Expected to Reach USD 16.7 Billion Globally in 2018 Market Research Reports” -PR.com Press Release 2013

5. Why Synthetic Biology? Detection Easy to Grow and Manufacture Training and Expertise Time and Transportation Remediation Extreme conditions Evolutionary Design Synthetic biology contains the answer!

6. Examples of Synthetic Biology Waste To Value Added Chemicals Detection of Contaminants Carbon Capture Biomaterials

7. How Can Synthetic Biology Affect Our Industry?

8. Most Awards Since 2003 Top 16 Team Worldwide How Can Synthetic Biology Affect Our Industry?

9. F ield R eady E lectrochemical D etector Patent Pending Technology How Can Synthetic Biology Affect Our Industry?

10. FRED – Field Ready Electrochemical Detector

12. Tailings Water: -200 million L/day -Seen from space -Corrosive & Toxic Tailing Ponds

13. The Target Market

15. FREDsenseThe Other Guy What Is In Your Water? Portability Speed Cost x3 x1 $25,000 / $250$2,500 / $150 Why Do They Care

16. Spot Test Portable Spot Test Multiple Array Testing Continuous Automated Multiple Compound Testing Real-Time Monitoring Solutions 6 Month Monitoring Solution Single or Multiple Parameters As low as 1 hour per reading ~4,320 Readings Per Device All customizable cartridges use a single detector

17. How Can the Technology be Applied FREDsense Provides A Solution To… Surface Water Monitoring Ground Water Monitoring Assessments and Baselines Remediation of Sites Long-term Monitoring

18. General Toxin Sensing Fracking -1.1 Million Wells in North America -1% Market Capture -$40 M Potential Revenue Other Related Markets -Sewage Treatment -Pharmaceuticals -Direct to Consumer Government regulated monitoring Other Market Opportunities

19. Synthetic Biology Based Tech Fast, portable, inexpensive Modular and customizable Opportunity