1. Organic Chemistry and 6 th edition Paula Yurkanis Bruice Chapter 28 The Chemistry of the Nucleic Acids Brian L. Groh Minnesota State University, Mankato Mankato, MN Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

2. Which of the following sets of bases distinguish DNA from RNA? 28.1 A.Adenine and thymine B.Thymine and cytosine C.Uracil and adenine D.Thymine and uracil E.Uracil and guanine Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

3. Which of the following sets of bases distinguish DNA from RNA? 28.1 A.Adenine and thymine B.Thymine and cytosine C.Uracil and adenine D.Thymine and uracil E.Uracil and guanine Thymine is unique to DNA while uracil is unique to RNA. Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

4. Which of the following are known as purines?28.1 A.Adenine and thymine B.Adenine and guanine C.Cytosine and uracil D.Thymine and cytosine E.Guanine and cytosine Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

5. Which of the following are known as purines?28.1 A.Adenine and thymine B.Adenine and guanine C.Cytosine and uracil D.Thymine and cytosine E.Guanine and cytosine The pyrimidine bases are cytosine, uracil, and thymine. Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

6. How many hydrogen bonds exist between the thymine and adenine base pairs? 28.3 A.1 B.2 C.3 D.4 E.5 Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

7. How many hydrogen bonds exist between the thymine and adenine base pairs? 28.3 A.1 B.2 C.3 D.4 E.5 Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

8. What is the sequence of bases in the strand of DNA that is complimentary to that shown? 5'-G-T-C-A-C-3' 28.7 A.5'-G-T-G-A-C-3' B.3'-G-T-G-A-C-5' C.5'-C-A-C-T-G-3' D.3'-C-A-C-T-G-5' E.3'-C-U-A-T-G-5' Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

9. What is the sequence of bases in the strand of DNA that is complimentary to that shown? 5'-G-T-C-A-C-3' 28.7 A.5'-G-T-G-A-C-3' B.3'-G-T-G-A-C-5' C.5'-C-A-C-T-G-3' D.3'-C-A-C-T-G-5' E.3'-C-U-A-T-G-5' The DNA strands in the double helix run in opposite directions. The 5' end of the complementary strand will be next to the 3' end of the strand. Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

10. The AGC codon on mRNA codes for which amino acid? 28.9 A.Arg B.Asp C.Gln D.Ser E.Thr Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

11. The AGC codon on mRNA codes for which amino acid? 28.9 A.Arg B.Asp C.Gln D.Ser E.Thr Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

12. A technique utilizing strand separation, base-pairing of primers, heat stable polymerases, and thermal cycling is 28.13 A.DNA sequencing B.Genetic engineering C.PCR D.Pyrosequencing E.Transcription Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.

13. A technique utilizing strand separation, base-pairing of primers, heat stable polymerases, and thermal cycling is 28.13 A.DNA sequencing B.Genetic engineering C.PCR D.Pyrosequencing E.Transcription PCR is a common technique that allows the rapid amplification of very small quantities of DNA Bruice: Organic Chemistry, © 2011 Pearson Education, Inc.