1. Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg June 30, 2008

2. Two F 2 populations produced DNA extracted - Qiagen DNeasy 96 plant kit AFLP fingerprinting technique Genomic DNA digested - PstI and MseI BSA performed - 256 PstI+MseI primer pairs PCR ABI 3100 - Genetic Analyzer A genetic map produced - JoinMap Materials and Methods

3. 135 150 160 200 250 300 340 350 400 370 M RP SP Primers: P-acg+M-cga Results F 2 population from CN56979/CN57130 4 90 56 82

4. Results - Mapping PaatMccg150 PaaaMcga2852 PaaaMcga5 PaggMcaa1558 PaggMcaa20515 PaggMcaa19016 PaccMcgg23022 PacgMcga37028 Sr_5713037 PaagMctc14062 PaagMctc13063 PaaaMcga33064 PaagMctc11066 PacgMcga12071