1. Introduction to the viruses

2.  Vaccinations  Cowpox ◦ cross protection against small pox  Variola virus  Major  Blisters  Blindness  Death  Minor  Poxviridae  dsDNA

3.  Define  Classificatio n ◦ Group  NA ◦ Family  -viridae ◦ Genus  -virus ◦ Species  Name

5.  Specific ◦ Host ◦ Cell type  General  Examples ◦ Bacteriophage ◦ Viroid (plant)

10.  Culture ◦ Bacteria ◦ Chick Embryos ◦ Cell/Tissue  Purified  Assays ◦ Hemagglutination ◦ Plaque

12.  Acellular  Infectious  Phases ◦ Extracellular ◦ Intracellular  Viron structure ◦ Nucelocapsid  Capsid  NA ◦ +/- Envelope

13.  Size ◦ 10-400 nm  Structural components ◦ Nucleocapsid ◦ Envelope (+/-)  Classifications ◦ NA ◦ ss or ds

14. Size

15.  Function ◦ Protect NA ◦ Aids in transfer to host  Structure ◦ Protein coat ◦ Capsomere arrange  Helical  Polyhedral  Complex

19.  Presence ◦ Enveloped ◦ Naked (non-enveloped)  Location ◦ Surrounds capsid  Source ◦ Host plasma membrane ◦ Nuclear membrane ◦ Endoplasmic reticulum  Components ◦ Phospholipid ◦ Proteins ◦ Glycoprotein spikes (+/-)  Examples ◦ Influenza ◦ Rabies ◦ Herpes ◦ HIV

21.  DNA OR RNA  Shape ◦ Circular ◦ Linear  Number ◦ One ◦ Or more  Strands ◦ ss ◦ ds ◦ + or - if RNA

22.  dsDNA ◦ Small pox ◦ Herpes ◦ Papilloma  ssDNA ◦ Parvo  dsRNA ◦ Rotavirus  +ssRNA ◦ Polio ◦ Rhino ◦ Corona  -ssRNA ◦ Measles, mumps ◦ Rabies ◦ Influenza  Retroviruses ◦ HIV

23.  Lytic ◦ Attach and entry ◦ Synthesis ◦ Assembly ◦ Lysis  Lysogenic ◦ Attach and entry ◦ NA in chromosome ◦ Binary fission ◦ Renters lytic phase  Synthesis  Assembly  Lysis

25. animation

27. Specialized Transduction

29. Phage Cycle Summary

32.  Attach  Entry ◦ Direct Penetration ◦ Membrane fusion ◦ Endocytosis  Uncoating  Synthesis  Assembly  Release

34. Direct

36. Endosome formation pH drop due to H+ pump Fusion peptide to PM Conformational change Release of NA Adenovirus uncoating Influenza Virus

37.  dsDNA: usual replication (for most)  ssDNA ◦ complementary strand ◦ Normal replication  dsRNA ◦ + strand = mRNA ◦ Template and copy  +ssRNA ◦ + strand = mRNA ◦ Complimentary strand for template  -ssRNA ◦ Viral enzymes make + strand ◦ Template for mRNA and -ss  Retroviruses ◦ +ssRNA (mRNA to make DNA) ◦ Reverse transcriptase ◦ DNA is template for new +ssRNA

42.  DNA ◦ Nucleus ◦ Moves to cytoplasm  RNA ◦ cytoplasm

43. Herpes

44.  Types ◦ Budding  Acquire membranes  envelope ◦ Exocytosis ◦ Lysis  Latency

49. Viral Transmission

51. Viral Infections

53.  Definitions ◦ Oncogenes ◦ Activation  Mutation  Transduction  Tumor ◦ Types  Benign  Malignant ◦ Characteristics  Examples ◦ DNA  Adenovirus  Herpes  Poxviruses  Papoviruses  Hepadenaviruses ◦ RNA  Retroviruses  HIV  HTLV

54. Adaptive Immunity

55.  Attachment antagonists ◦ Block attachment molecule  Arildone  Inhibit Uncoating ◦ Neutralize acid environment  Amantadine  Rimantadine  Inhibit DNA/RNA synthesis ◦ Activation by phosphorylation of drug by viral kinases  Acyclovir  Gancyclovir

56.  Infectious proteins  Structure ◦ Insoluble ◦ Beta pleated sheet  PrPc  PrPsc  Affects NS ◦ Fibrils ◦ Vacuoles (spongy)  Examples ◦ BSE ◦ CJD ◦ Scrapie ◦ Kuru

58.  ALZ  MS  Parkinsons  ALS  DM  RA  Hepatitis  SLE  Neoplasia

60. Questions?