1. Histology Laboratories James D. Jamieson, MD/PhD
Molecules to Systems
2003
Compiled by
James D. Jamieson, MD/PhD
Thomas L. Lentz, MD
No part of this image collection may be distributed outside of the Yale University Intranet.

2. Acknowledgements Sources of Micrographs, Diagrams and Figures
Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002.
Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, 1994.
Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999.
Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, 1979.
Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971.
Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York,
Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo.
Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, 2000.
Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz.
Web Resources
Cushing Library Educational Software/Cell Biology/Several Histology Resources

3. Endocrine Systems Laboratory

4. Anterior Pituitary

6. Regulation of Synthesis
of Pituitary Hormones

7. Optic chiasma
Hypothlamus
Posterior pituitary
Stalk
Anterior pituitary
Pituitary: Sagittal Section
Sella turcica

8. Acidophils (Mammotroph, PRL
Somatotroph, GH)
Chromophobes
Basophils
(Corticotrophs,ACTH
Thyrotroph, TSH
Gonadotroph, FSH, LH)
Anterior Pituitary; H&E.

9. Fenestrated capillary
Anterior Pituitary TEM
S: somatotroph
G: gonadotroph

10. Posterior Pituitary

12. Herring bodies
Blood vessels
Glial cells
Posterior Pituitary

13. ADH/Oxytocin Release in Posterior Pituitary

14. Adrenal Cortex

15. Adrenal
C: Cortex
M: Medulla

16. Blood Supply of Adrenal

17. Capsule ZG ZF
Adrenal Cortex: Zona Glomerulosa; aldosterone

18. ZF
Adrenal Cortex: Zona Fasciculata; cortisol

19. ZR
Adrenal Cortex: Zona Reticularis; weak androgens

20. Adrenal Cortex (ZR) and Medulla (M)

21. Adrenal Cortical Cell

22. Adrenal Medulla

23. Blood vessel
Chromaffin cells
Adrenal Medulla; epinephrine and norepinephrine

24. Sympathetic nerve fibers
Chromaffin cells with epinephrine granules
Adrenal Medulla

25. Thyroid/Parathyroid

26. Follicles
Thyroid Gland

27. Follicular epithelial cells
Colloid (thyroglobulin)
Thyroid Follicles

28. Follicular epithelial cells
Parafollicular cells/C-cells
Thyroid Follicles; C cells: calcitonin, lowers blood Ca++

29. Colloid
C cell
Thyroid Follicle TEM

30. Parathyroid

31. Parathyroid
Thyroid
Parathyroid; PTH elevates Blood Ca++

32. Chief (PTH) cells
Adipose cells
Oxyphil cells
Parathyroid

33. Oxyphil cells
Chief (PTH) cells
Parathyroid

34. Endocrine Pancreas

35. Exocrine pancreas
Islets of Langerhans
Endocrine Pancreas

36. Exocrine acini
Blood vessels
Vein
Endocrine Pancreas

37. Normal Islet. Immunoperoxidase against glucagon (L) and insulin (R)

38. Islet Immunostained for Insulin

39. Same Islet Immunostained for Glucagon

40. Glucose Homeostasis: Liver and Endocrine Pancreas

41. GI Endocrine System

42. Enteroendocrine Cells: stained for serotonin

43. Gut Endocrine Cells: XS crypt
A: endocrine cells facing lamina propria

44. Lumen of gastric gland
Lamina propria
Gastric Endocrine Cells: G = gastrin cell

45. Mucosal Neuroendocrine Cells Facing Lumen and Lamina Propria

46. Mucosal Neuroendocrine Cells Facing Lamina Propria Only
Gut Endocrine Cells Facing Lumen and Lamina Propria

47. A Bit of Pathology

48. Thyroid: Graves disease
Thyroid: Graves disease. What is the functional state of the epithelial cells and the colloid? This path. Is due to autoantibodies that mimic TSH. Discuss in terms of the cell biology of the thyroid.

49. Nodular goiter. Thyroid from a person living in Switzerland
Nodular goiter. Thyroid from a person living in Switzerland. What do you see that is different from normal?

50. Hashimoto’s thyroiditis
Hashimoto’s thyroiditis. Note abnormal lymphoid follicle and infiltrating lymphocytes. What might be the cause of this condition?

51. Insulinoma (malignant): Islet cell tumor stained with insulin antibody
Insulinoma (malignant): Islet cell tumor stained with insulin antibody. What symptoms might the tumor produce?

52. Islet adenoma (benign - encapsulated) compared to normal islet (R).

53. Normal parathyroid (L); functional adenoma (R)
Normal parathyroid (L); functional adenoma (R). What might you see on bone density scans?

54. Normal anterior pituitary (L), Adenoma (R)
Normal anterior pituitary (L), Adenoma (R). This tumor is often functional and secretes prolactin. What might symptoms be in a male?

55. Anterior Pituitary: Immunoperoxidase for prolactin; used in diagnosis of functional pituitary adenomas

56. Capsule
Adrenal cortical adenoma. Cells resemble those of the zona fasciculata - lots of fat droplets. How might this condition affect this patient’s muscle mass. What hormone would you ask for in a lab test?