1. Discover the UniProt Blast tool

5. Murcia, February, 2011Protein Sequence Databases Customize the BLAST results

6. Look for the local alignment

7. Highlight important positions within the alignment according to the sequence annotation The metal binding sites are conserved

8. HBB_HUMAN

9. Discover the UniProt Align tool

10. 1- Select sequences of interest… (from Blast results for example ) 2 - Align…

11. Add the ‘query’ sequence (HBB_HUMAN)

12. Conservation of the metal-binding site…. Highlight important positions within the alignment according to the sequence annotation

13. Compare the Blast @ UniProt and @ NCBI

14. Protein Sequence Databases The ‘alternative’ sequence(s) not ‘directly available’ for a lot of tools, including protein identification tools, Blast, depending on the server !…. Murcia, February, 2011

15. Blast P04150 against Swiss-Prot / homo sapiens @ UniProt Isoform sequences Murcia, February, 2011Protein Sequence Databases

16. Blast P04150 against Swiss-Prot / homo sapiens @ NCBI The isoform sequences (from Swiss-Prot) are not present in the NCBI protein database ! The.x number (P06401.4) correspond to the version number of the sequence…not to an alternatively spliced sequence ! Murcia, February, 2011