1. www.fludb.org Influenza Research Database (IRD) 26 September 2011 Richard H. Scheuermann, Ph.D. Department of Pathology U.T. Southwestern Medical Center

2. www.fludb.org History of IRD SEP2004 APR2007 DEC2009

3. www.fludb.org CEIRS Network

4. www.fludb.org Influenza Virus Orthomyxoviridae family Negative-strand RNA Segmented Enveloped 8 RNA segments encode 11 proteins Classified based on serology of HA and NA

5. www.fludb.org Public Health Impact of Influenza Seasonal flu epidemics occur yearly during the fall/ winter months and result in 3-5 million cases of severe illness worldwide. More than 200,000 people are hospitalized each year with seasonal flu-related complications in the U.S. Approximately 36,000 deaths occur due to seasonal flu each year in the U.S. Populations at highest risk are children under age 2, adults age 65 and older, and groups with other co-morbidities. Pandemics – 1918 Spanish flu (H1N1); 20 - 100 million deaths – 1957 Asian flu (H2N2); 1 - 1.5 million deaths – 1968 Hong Kong flu (H3N2); 750,000 - 1 million deaths – 2009 Swine origin (H1N1); > 16,000 deaths as of March 2010 Source: World Health Organization - http://www.who.int/mediacentre/factsheets/fs211/en/index.html

6. www.fludb.org IRD Overview www.fludb.org

7. Data Summary

8. www.fludb.org Data Sources

9. www.fludb.org Search Access to Data www.fludb.org

10. Data Types

11. www.fludb.org Analysis and Visualization www.fludb.org

12. Analysis and Visualization Tools

13. www.fludb.org Workbench Access www.fludb.org

14. Data Submission www.fludb.org

15. Data Submission Page

16. www.fludb.org DATA INTEGRATION

17. www.fludb.org 3D Structure Viewer

18. www.fludb.org UNIQUE COMMUNITY DATA

19. www.fludb.org Surveillance Search

20. www.fludb.org

26. Google Maps Display

27. www.fludb.org UNIQUE IRD DATA

28. www.fludb.org SFVT

29. www.fludb.org SF definition SF can be defined for any region of interest because of structural or functional properties Defined based on experimentation reported in the literature, 3D protein structures (PDB records), UniProt annotations and IEDB epitope records Defined by the specific amino acid positions in the polypeptide chain Manually curated and externally validated

30. www.fludb.org SF Counts

31. www.fludb.org NS1 Functional SFs

32. www.fludb.org SF Details

33. www.fludb.org VTs

34. www.fludb.org VT-specific Strain List

35. www.fludb.org IRD Summary Funded by U.S. National Institute of Allergy and Infectious Diseases (NIAID) Free and open access with no use restrictions Developed by a team of research scientists, bioinformaticians and professional software developers Comprehensive collection of public data Novel derived data, novel analytical tools, unique functions Integration – Integration – Integration www.fludb.org

36. Challenges and Future Directions Inconsistent annotations Competition with other influenza-focused resources GISAID collaboration Host systems biology and “omics” data

37. www.fludb.org 37 U.T. Southwestern – Richard Scheuermann (PI) – Burke Squires – Jyothi Noronha – Victoria Hunt – Shubhada Godbole – Brett Pickett – Yun Zhang MSSM – Adolfo Garcia-Sastre – Eric Bortz – Gina Conenello – Peter Palese Vecna – Chris Larsen – Al Ramsey LANL – Catherine Macken – Mira Dimitrijevic U.C. Davis – Nicole Baumgarth Northrop Grumman – Ed Klem – Mike Atassi – Kevin Biersack – Jon Dietrich – Wenjie Hua – Wei Jen – Sanjeev Kumar – Xiaomei Li – Zaigang Liu – Jason Lucas – Michelle Lu – Bruce Quesenberry – Barbara Rotchford – Hongbo Su – Bryan Walters – Jianjun Wang – Sam Zaremba – Liwei Zhou IRD SWG – Gillian Air, OMRF – Carol Cardona, Univ. Minnesota – Adolfo Garcia-Sastre, Mt Sinai – Elodie Ghedin, Univ. Pittsburgh – Martha Nelson, Fogarty – Daniel Perez, Univ. Maryland – Gavin Smith, Duke Singapore – David Spiro, JCVI – Dave Stallknecht, Univ. Georgia – David Topham, Rochester – Richard Webby, St Jude USDA – David Suarez Sage Analytica – Robert Taylor – Lone Simonsen CEIRS Centers Acknowledgments N01AI40041