1. Pierre Jaccard1 Chl-a Fluorescence Measurements 10.06.2013 - MyOcean Tutorial, NERSC, Bergen

2. Pierre Jaccard2 Differences from Physical Sensors Living species Measurement principle differs Chl-a Fluoresence is not Chl-a Proxy Neither really bad or good Rather better or worse Correct values from HPLC

3. Fluctuations in CHLA FL / CHLA CONC Problem for using Chla_fl as phytoplankton biomass estimation Search for “solutions” in Ferrybox data series and other dataset/time-series from other investigations using data on: – Sun light – Day length – Temperature – Species composition –... K. Sørensen, M. Norli and A. Folkestad 3

4. Measurements In Practice 10.06.2013 - MyOcean Tutorial, NERSC, Bergen Pierre Jaccard4

5. Fluorescence high at small concentrations Fluorescence low at high concentrations Ferrybox Chl_a time-series 2002-2008 No yearly calibration of the fluorescence Better match at high concentrations With yearly calibration of the fluorescence K. Sørensen, M. Norli and A. Folkestad5

6. Most months show the same trend: High ratio at night Lower ratio at daytime FerryBox seasonal and night and day variation Yearly calibration of the Chl-a fluorescense using all the Chl- a_hplc water samples K. Sørensen, M. Norli and A. Folkestad6

7. Variation of Chl-a_Fl/Chl-a for algal culture Nr.Algekulturer 1Chrysochromulina polylepis 2Dunaliella tertiolecta 3Emiliania huxleyi 4Oscilatoria agardii 5Prorocentrum minimum 6Prymnesium parvum 7Phaeodactylum tricornutum 8Selenastrum capricornutum 9Skeletonema costatum Algal culture Chl-a_Fl/Chl-a Determined during dark adapted algal from a exponetial growing algal culture Factor 6 all species tested Factor 2.5- 3 for Norw. waters K. Sørensen, M. Norli and A. Folkestad7

8. Validation of the chlorophyll-a fluorescence with monitoring data K. Sørensen, M. Norli and A. Folkestad8