1. Herbicide Residues in Fish from McGarvey and Blue Creeks California Department of Fish and Game Pesticide Investigations Unit Rancho Cordova, California

2. Background Information n Study concept under discussion for 2 yrs n Work plan (sample collection, sample custody, study design) n Contract between DPR and DFG for analysis of phenoxy-herbicides and degradates n Samples collected in mid-April to mid-May n Sculpin from McGarvey Cr and trout from WF Blue Cr n 2,4-D and dichlorphenol n Triclopyr and trichloropyridinol

3. Accelerated Solvent Extractor n Heat, pressure and solvent n Extract pesticide from tissue n Other junk too

4. Gel Permeation Chromatograph n Remove fat from ASE samples n Clean-up n Leaves solvent and pesticide

5. Gas Chromatograph n Analysis instrument n Sample is super- heated to gas n A solid adsorbent causes partitioning n Band for each compound

6. Gas Chromatorgaphy Mass Spectrometry n Interface GC w/ MS n MS charges particles n Spectrum of parent ion and fragments n Unique to compound n Separate w/ GC n Identify w/ MS

7. Quality Control

8. Herbicide Residues in Fish 1 = < 1 ppb 2 = < 5 ppb 3 = < 2 ppb