1. Wheat CAP: Mapping of Wheat Quality E. Souza - USDA-ARS, Wooster C. Sneller, M. Guttieri, N. Smith – Ohio State University Acknowledgments Jorge Dubcovsky, UC Davis, – Project Coordinator Jamie Sherman, Montana State Univ. – Outreach Coordinator Carl Griffey, Virginia Tech – Eastern US Coordinator Duc Hua, Ohio State – Technical flour analysis Anne Sturbaum, USDA-ARS, Wooster – Association Mapping Gina Brown-Guediera, USDA-ARS Raleigh – Genotyping support Mapping Project Leaders J. Johnson – GA, C. Griffey – VA, H. Ohm, - IN, M. Sorrells – NY, C. Sneller - OH

3. Wheat Coordinated Agricultural Project Comprehensive mapping effort for wheat characteristics of economic value Manipulation of previously mapped or marked genes for the development of new varieties

4. Quality Traits for Marker Assisted Selection Gluten Strength –HMW Glutenins Glu-A1, Glu-B1, Glu-D1 –LMW Glutenins Glu-A3 –Gliadins Gli-D1 –Rye Translocations 1B/1R, 1A/1R Protein Concentration –Gpc-1 Grain Hardness –Softer Hard Wheat PinA => PinB on 5D –Multiple loci for softness – Pina-A m 1 on 5A

5. Quality Traits for Marker Assisted Selection Pre-harvest sprouting –Phs-1 –Vp1 Semolina color –Lr19 rust resistance linked to yellow pigment Starch structure –Amylose reducing (Waxy) Wx-1A, Wx-1B, and Wx-1D

6. Effect of Glutenin and Rye Translocations in an Association Mapping Population with Genetic State Determined by Markers 187 Soft winter wheat cultivars grown in 7 environments, 2007-2008. Background genetic effects adjusted by genetic similarity using DArT markers Glu-1D a (2+12) Glu-1D d (5+10) Lactic acid 111.8%118.3% No. of Cultivars 12551 1BL:1RS Rye Transloc. 1BL:1BS No Transloc. Lactic acid 103.3 %114.6 % No. of Cultivars 12174 Difference sig. @ p<0.0015Difference sig. @ p<0.0004

7. PIPedigreeSizeMarkers# of Envs SorrellsFoster/Kanqueen90+254 SSR and AFLP (DArTs added) 3 (NY, OH) Ohio StateFoster/PIO 25R26175163 SSR4 (OH, NY) JohnsonSS 550/PIO 26R46150153+ SSRs4 OH 2 GA to do GriffeyJaypee/USG 3209125220 SSR, 363 DArT5 (VA,NC) Ohm92201/91193190383 DArT and SSR4 (IN, NY, OH) SorrellsCayuga/Caledonia155256 SSR and AFLP (DArTs added) 4 for Starch, 2 for others Ohio State, USDA SWQL Association Mapping: Historical set 187~600 DArTs, SSRs, others Populations & Current Phenotyping & Genotyping

8. Water absorption Starch damage Pentosans Flour Yield Gluten strength Softness Equivalent (SE) (break flour yield) Flour Protein (PRO) Test Weight (TW) Key Components of Soft Wheat Quality Measured By OSU ACT LIKE ONE TRAIT Fl. Yield Water Pentosan Starch Dam. r = -0.60, -0.90

9. 42 Trait/Population combinations Summary of R 2 (x100) for 218 QTL 58% QTL < 10% 28% QTL 10-20% 14% QTL > 20%

10. R 2 (x100), Location, Population of Three Largest Effect QTL 1 st 2 nd 3 rd Protein26%, 1BL, 9/925%, 2BS, F/K24%, 2BS, F/25 Glu Strgth38%, IBCL, F/K32%, 1BCL, F/2531%, 1BCL, S/26 Water Abs27%, 6BC, S/2625%, 1BL, 9/925%, 1BL, F/K Starch Dam57%, 2BS, F/K28%, 1BCL, J/U28%, 1BL, 9/9 Pentosans25%, 1BCL, J/U24%, 2BS, F/2522%, 6BC, S/26 Fl. Yield30%, 1BCL, F/2527%, 2BS, F/K16%, 2BS, F/25 Soft Eq.23%, 2BS, F/K20%, 5AS, F/K16%, 3BS, S/26 10/21 QTL from 1B 7/21 from 2BS

11. R 2 (x100) of QTL on 2B Short Arm Fo/KFo/25S/26Ja/U9/9Ca/C Protein252419 Glu Strgth95 Water Abs182119 Starch Dam57 † 7111012 Pentosans1724878 Fl. Yield27168 Soft Eq.23135 = not significant † 90 lines

12. Association Mapping Population of 187 Soft Winter Wheat Cultivars, 2007 & 2008 Grown in IN, KY OH, NY, and VA

13. Effect of Milling QTL Chromosome 2B in an Association Mapping Population – XBarc10 marker 187 Soft winter wheat cultivars grown in 7 environments, 2007-2008. Background genetic effects adjusted by genetic similarity using DArT markers Barc10 303 bp Barc10 297 bp or 300 bp Break Flour (Softness equiv.) 56.5%58.8% No. of Cultivars 8791 Barc10 303 bp Barc10 297 bp or 300 bp Break Flour (Allis Mill) 30.7 %33.5 % No. of Cultivars 8791 Difference sig. @ p<0.0007Difference sig. @ p<0.0001

14. Effect of Dwarfing and Photoperiodism in an Association Mapping Population with Genetic State Determined by Markers 187 Soft winter wheat cultivars grown in 7 environments, 2007-2008. Background genetic effects adjusted by genetic similarity using DArT markers TallSemi- dwarf Rht1 or 2 Break Flour (Softness equiv.) 56.1%58.8% No. of Cultivars 70112 P-period Insensitive P-period Sensitive Break Flour (Softness equiv.) 57.7 % No. of Cultivars 46129 Non-significant differenceDifference sig. @ p<0.0001

15. Discussion Points In soft wheat many of the quality traits are positively associated in terms of desirability – e.g. greater flour yield and lower water absorption Quality traits in all mapping populations are highly heritable Most of the quality traits are complex – multiple places in the genome