1. Fundamentals of Biochemistry Third Edition Fundamentals of Biochemistry Third Edition Chapter 11 Enzymatic Catalysis Chapter 11 Enzymatic Catalysis Copyright © 2008 by John Wiley & Sons, Inc. Donald Voet Judith G. Voet Charlotte W. Pratt

2. Section 1 – General Properties of Enzymes High reaction rates Milder reaction conditions Greater reaction specificity Capacity for regulation

4. Stereospecificity

5. Specificity of enzymes

8. Section 2 – Activation Energy and Reaction Coordinate

9. Section 3 Catalytic Mechanisms Acid-base catalysis Covalent catalysis Metal ion catalysis Proximity and orientation effects Preferential binding of the transition state complex

10. General Acid – Base Catalysis

12. Box 11-1

14. Bovine Pancreatic RNase A

15. Covalent Catalysis

18. Metal Ion Catalysis Bind to substrate and orient them Mediate Redox reactions Stabilize or shield negative charges

19. Carbonic Anhydrase

21. How enzymes facilitate reactions Enzyme brings substrates close together (x5) Enzymes orient the substrates (x100) Stabilize transition state Eliminate translational and vibrational motion (10 7 )