1. Colorimetric of aspirin determination

2. antiplatelet effect by inhibiting the production of thromboxane Acetyl salicylic acid

3. determine the amount of aspirin in a commercial aspirin product. used to determine the purity of the aspirin produced in the Microscale Synthesis of Acetylsalicylic Acid lab. purposes

4. principle Exx NaOH ∆ + FeCL 3 Neutralize Why? Neutralize Why? To prevent form Fe(OH)3 By what ? Conc.HCL By what ? Conc.HCL Na acetate Na salicylate

5. procedure

6. Pipet a 5 mL of standard solution to a 100 mL volumetric flask. then add 5 ml of FeCL3 solution then complete to market with distilled water. Label the flask.

8. Pipette 5ml of sample solution to 100 ml volumetric flask. Add 5 ml of 5% FeCL3 solution then complete with distilled water To the market. ( mix well). Label the flask. Put 5 ml of FeCL3 to 100ml volumetric flask then complete the volume by distilled water

9. Standard sample blank

11. For sample Calculated Conc. Practical Conc. = 2 mg /100ml (mg%) 1-beers law. 2-matching point.