1. MOLECULAR BIOLOGY – Protein synthesis PROTEIN SYNTHESIS

2. Figure 6-50 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

3. Figure 6-51 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

4. Figure 6-52 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis tRNA

5. Figure 6-53 Molecular Biology of the Cell (© Garland Science 2008) Some tRNA can read more than one codon minimum set of tRNA is 31 (for 61 codons) I... inosine (third base not so squeezed as in double helix) MOLECULAR BIOLOGY – Protein synthesis

6. Figure 6-58 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

7. Figure 6-56 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis (Aminoacyl-AMP) Charging tRNA

8. Figure 6-61 Molecular Biology of the Cell (© Garland Science 2008) amino (N-) terminus carboxyl (C-) terminus peptide bond MOLECULAR BIOLOGY – Protein synthesis

9. Figure 6-63 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

10. 16S rRNA secondary structure rRNAs not ribosomal proteins responsible for most of the reactions: 23S rRNA – ribozyme peptidyl transferase MOLECULAR BIOLOGY – Protein synthesis

11. Figure 6-64 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

12. Prokaryotes nnnnnnAGGAGGUnnnnnnnAUGnnnnnnn UCCUCCA Shine-Delgarno sequence Start FormylMethionin 16S rRNA Shine-Delgarno sequence MOLECULAR BIOLOGY – Protein synthesis

13. Figure 6-72 (part 2 of 5) Molecular Biology of the Cell (© Garland Science 2008) Eukaryotes MOLECULAR BIOLOGY – Protein synthesis GCCRCCAUGG surrounding sequence important

14. Figure 6-72 (part 3 of 5) Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

15. Figure 6-66 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

16. Figure 6-67 (part 1 of 7) Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

17. Figure 6-67 (part 6 of 7) Molecular Biology of the Cell (© Garland Science 2008) TRANSLOCATION

18. Coupled transcription-translation in bacteria MOLECULAR BIOLOGY – Protein synthesis

19. Figure 6-76 Molecular Biology of the Cell (© Garland Science 2008) Eukaryotes MOLECULAR BIOLOGY – Protein synthesis

20. Figure 6-79 Molecular Biology of the Cell (© Garland Science 2008) MANY ANTIBIOTICS WORK BY INHIBITING BACTERIAL PROTEIN SYNTHESIS MOLECULAR BIOLOGY – Protein synthesis

21. Table 6-4 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

22. Exceptions to universal genetic code Endosymbiotic origin of mitochondria and chloroplasts MOLECULAR BIOLOGY – Protein synthesis

23. PROTEIN STRUCTURES AND FUNCTIONS MOLECULAR BIOLOGY – Protein synthesis

24. Figure 3-1 (part 1 of 2) Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

25. Figure 3-2 Molecular Biology of the Cell (© Garland Science 2008) HydrophylicHydrophobic MOLECULAR BIOLOGY – Protein synthesis

26. Primary, Secondary, Tertiary and Quaternary Protein Structure MOLECULAR BIOLOGY – Protein synthesis

27. Figure 3-4 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

28. Figure 3-5 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

29. Figure 6-82 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

30. Figure 6-86 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis Chaperonins/Chaperons needed for proper folding Hsp … heat shock proteins

31. Figure 6-87 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

32. Protein glycosylation MOLECULAR BIOLOGY – Protein synthesis N-linked glycosylation (in ER) O-linked glycosylation (in golgi) Proper folding Cell-cell adhesion O-linked serine N-linked asparagine

33. Figure 6-88 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

34. Protein degradation in protesome MOLECULAR BIOLOGY – Protein synthesis „ubiquitination“ by ubiqutin ligase

35. Signal sequence – some proteins have addresses secreted proteins, mitochondrial etc. MOLECULAR BIOLOGY – Protein synthesis

36. PROTEIN FUNCTIONS IN CELL Structural proteins Cytoskeleton Extracellualr matrix Mechanical proteins actin, myosin Enzymes Binding proteins transport, storage Information processing Proteins receptors, signals MOLECULAR BIOLOGY – Protein synthesis

37. Table 3-1 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

38. Figure 3-64 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

39. Figure 3-66 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

40. Figure 3-73 Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

41. Figure 3-81a Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis

42. Figure 3-81c Molecular Biology of the Cell (© Garland Science 2008) MOLECULAR BIOLOGY – Protein synthesis