1. Word of the Day: Enzyme: An enzyme is a substance that starts a chemical reaction in cells EnzymeChemical acted upon Product lipaseFats and oilsGlycerol and fatty acids ProteaseProteinsAmino acids CatalaseHydrogen peroxide Water and oxygen

2. What does your spit do to food? Amylase Lab

3. Background Amylase is an enzyme found in your saliva Amylase breaks down starches and turns them into sugars

4. Remember your Latin and Greek Starch -------------> glucose + glucose + glucose +… Amylase enzyme Found in saliva Substrate Type of starch Product Type of sugar which your body uses for energy

5. Let’s see how it really works… Step 1: Preparing the Lab –Get 10 test tubes –Label 1-10 –Add 5 drops of iodine to each test tube 1 2 dropper fulls of iodine 1

6. Step 2: Prepare the saliva solution Collect 4 ml of saliva - –Spit through the straw into a clean graduated cylinder –Add 17 ml of water to graduated cylinder and mix. Use straw to collect saliva

7. Step 3: Prepare the Starch Solution Using a 2nd graduated cylinder, get 7ml of starch solution and pour into a small beaker Measure 7ml of starch solution into beaker

8. Step 4: Mix the saliva solution into the starch solution Record the exact time of mixing Saliva solution Starch solution

9. Step 5: Add Saliva solution to Test Tube #1 Add 10 drops of saliva/starch to test tube # 1 Record color: –Black/Purple: starch present –Yellow/Brown: no starch present

10. Step 6: Repeat Step 5, Every Three Minutes Every three minutes, add 10 drops of saliva-starch solution to a new test tube –Record color –Repeat until the starch is no longer present

11. Data Table TimeStarch Present (Yes/No) 0 3 6 9 12 15 18

12. Claim, Evidence and Explanation Write a CEE Due Next Class