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Chapter 12 The Hitachi FMBIO II Fluorescence Imaging System ©2002 Academic Press.

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Presentation on theme: "Chapter 12 The Hitachi FMBIO II Fluorescence Imaging System ©2002 Academic Press."— Presentation transcript:

1 Chapter 12 The Hitachi FMBIO II Fluorescence Imaging System ©2002 Academic Press

2 FMBIO II Fluorescence Imaging System Gel placed here ©2002 Academic Press

3 Enlargement of Gel Image from FMBIO II Instrument ©2002 Academic Press

4 Sample Processing Steps with FMBIO II Pour Gel Load Samples (allelic ladder every third lane) Electrophoresis Pour Gel Load Samples (allelic ladder every third lane) Prepare samples: denature, cool, and mix with loading dye Electrophoresis Post- Electrophoresis ©2002 Academic Press

5 Sample Processing Steps (cont.) SCAN GEL Size DNA Fragments Genotype STR alleles FMBIO II Fluorescence Imaging System Perform Data Analysis FMBIO ® Analysis Software ReadImage Software Manually inspect the data ELECTROPHORESIS and DETECTION steps are separate STaR Call™ Genotyping Software ©2002 Academic Press

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