1. DNA and RNA Chapter 12-1 http://faculty.uca.edu/~johnc/mbi1440.htm http://www.wappingersschools.org/RCK/staff/teacherhp/johnson/visualvocab/mRNA.gif

2. GENETIC MATERIAL In the middle of the 1900’s scientists were asking questions about genes. What is a gene made of? How do genes work? How do genes determine characteristics of organisms?

3. DO PROTEINS CARRY THE GENETIC CODE? At the time most scientists believed that _________ had to be the molecules that made up genes. There were so many different kinds proteins and DNA seemed to be too monotonous... repeating the same ___ subunits. 4 proteins

4. SEE GRIFFITH’s EXPERIMENT 1928 – Frederick Griffith looked at pneumonia bacteria trying to figure out what made people die Images from: http://microvet.arizona.edu/Courses/vsc610/mic205/griffith.jpg S (SMOOTH) strain - killed mice R (Rough) strain -mice lived

5. If he heated the LETHAL strain first _______________ Images from: http://microvet.arizona.edu/Courses/vsc610/mic205/griffith.jpg The heat killed bacteria were no longer LETHAL.... mice lived.

6. BUT... If he mixed heat-killed LETHAL bacteria with live harmless bacteria ________________ Images from: http://microvet.arizona.edu/Courses/vsc610/mic205/griffith.jpg... mice DIED ! When he looked inside dead mice, he found ______________ bacteria! Somehow the heat killed LETHAL bacteria passed their characteristics to the harmless bacteria. LIVE LETHAL

7. See a video clip about GRIFFITH’S EXPERIMENTS (12A)(12A)

8. Griffith called this process __________________ because one strain of bacteria had been changed permanently into another. But what was the factor that caused the transformation? A protein ? A lipid ? A carbohydrate ? A nucleic acid ? TRANSFORMATION

9. 1944- Oswald Avery’s team of scientists repeat Griffith’s experiments looking for the transforming molecule. After heat killing the LETHAL Pneumonia bacteria, he treated them with digestive enzymes that destroy specific kinds of molecules. If proteins, polysaccharides, lipids, or RNA’s were destroyed... ______________________________ http://cystitis-cystitis.com/Images/testtube.jpg http://en.wikipedia.org/wiki/Oswald_Avery http://faculty.uca.edu/~johnc/mbi1440.htm Transformation still occurred!

10. But when they treated the heat-killed LETHAL bacteria with enzymes to destroy _____ there was NO transformation!... the mice lived! DNA was the molecule that caused the genetic change. DNA http://web.jjay.cuny.edu/~acarpi/NSC/12-dna.htm

11. GRIFFITH EXPERIMENT (PNEUMONIA-RAT) Showed ____________ could be passed between bacteria & cause a change. AVERY EXPERIMENT (Digestive enzymes) showed that the genetic material was _____ DNA genetic material

12. Scientists are skeptical… it takes more than one experiment to convince them. 1952-Alfred Hershey and Martha Chase experimented with viruses that infect bacteria = _________________ Knew bacteriophages were made of ________ and _______ bacteriophages http://www.mun.ca/biology/scarr/Chase_&_Hershey_1953.jpg Hear about their cool experiment proteins DNA

13. http://www.mun.ca/biology/scarr/hersheychase-experiment.jpg

14. HERSHEY-CHASE BLENDER EXPERIMENT Showed_______________ entered cell during infection. Conclusion: ______________in virus was _____ not protein only DNA not protein Genetic material DNA

15. BACTERIAL VIRUSES http://faculty.uca.edu/~johnc/mbi1440.htm

16. DNA is a DOUBLE HELIX http://en.wikipedia.org/wiki/Rosalind_Franklin http://www.time.com/time/time100/scientist/profile/watsoncrick.html X-ray experiments by Rosalind Franklin led James Watson and Francis Crick to the discovery of the structure of DNA in 1953

17. Hydrogen bonds Nucleotide Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G) Figure 12–7 Structure of DNA Section 12-1

18. NUCLEIC ACIDS are built from subunits called ____________________ Image by: Riedell NUCLEOTIDES SUGAR in DNA is ________________ deoxyribose

19. NITROGEN BASES in DNA _____________= A _____________ = G _____________ = C ______________ = T ADENINE GUANINE CYTOSINE THYMINE No URACIL

20. DEOXYRIBONUCLEIC ACID Image from: http://www.tokyo-med.ac.jp/genet/picts/dna.jpg ______________ STRANDED Backbone (sides of ladder) made of _____________ and _____________ DOUBLE PHOSPHATES sugars

21. Purines (2 rings) Pyrimidines (1 ring) A G C T Phosphate group Deoxyribose sugar Nitrogen bases =“Steps of ladder” © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

22. CHARGAFF’S RULES A - T G -C Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif ______ _________ At time no one knew why… now we know its because Adenine always bonds across with____________ Guanine always bonds across with ____________ THYMINE CYTOSINE

23. DOUBLE HELIX Hydrogen Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif _____________ bonds between nitrogen bases hold the two strands together.

24. Interest Grabber Answers 1.On a sheet of paper, draw a curving or zig-zagging line that divides the paper into two halves. Vary the bends in the line as you draw it. Without tracing, copy the line on a second sheet of paper. 2.Hold the papers side by side, and compare the lines. Do they look the same? 3. Now, stack the papers, one on top of the other, and hold the papers up to the light. Are the lines the same? 4.How could you use the original paper to draw exact copies of the line without tracing it? 5.Why is it important that the copies of DNA that are given to new daughter cells be exact copies of the original? Lines will likely look similar. Overlaying the papers will show variations in the lines. Use 1 st line as a template to draw the line on another sheet of paper. Each cell must have the correct DNA, or the cell will not have the correct characteristics.

25. CHROMOSOMES & DNA REPLICATION 12-2

26. Chromosome E. coli bacterium Bases on the chromosome Chromosome Structure in Prokaryotes © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved DNA molecule in bacteria is: ______________ Found in __________ (NO nucleus) SINGLE CIRCULAR CYTOPLASM Approximately 5 million base pairs 3,000 genes

27. DNA in EUKARYOTES is packaged into chromosomes Humans have approximately 3 billion base pairs (1 m long) 60,000 to 100,000 genes If the diameter of the DNA (2 nanometers) was as wide as a fishing line (0.5 millimeters) it might stretch as far as 21.2 km (or 13.6 miles) in length which would all have to be packed into a nucleus, the equivalent size of 25 cm in diameter. That is some packaging! http://www.paternityexperts.com/images/DNA-of-life.jpg

28. THINK ABOUT IT How could you get this piece of string into the container? http://www.artzooks.com/files/3966/AZ533823_320.jpg http://www.mivaroo.com/sites/toyconnection.com/

29. Chromosome Structure of Eukaryotes Chromosome Supercoils Coils Nucleosome Histones DNA double helix © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved DNA is: ______________ Found in __________ in multiple chromosome bundles nucleus

30. Chromosome Structure of Eukaryotes Nucleosome Histones DNA double helix © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Eukaryotic chromosomes are made of _____ & __________ called ___________ Together the DNA & histone proteins forms a bead-like structure called a ______________ DNA PROTEINS HISTONES NUCLEOSOME

31. Chromosome Structure of Eukaryotes Chromosome Supercoils Coils Nucleosome Histones DNA double helix © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved Nucleosomes pack together to form thick coiled fibers. When cell is NOT dividing, these fibers are spread out in nucleus as ___________. (Allows reading of code) CHROMATIN

32. Chromosome Structure of Eukaryotes Chromosome Supercoils Coils Nucleosome Histones DNA double helix © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved When cell gets ready to divide, the fibers pack even more tightly to form ___________.(Makes it easier to move DNA during mitosis) chromosomes

33. HOW IS DNA COPIED? Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif The structure of DNA explains how it can be copied. Each strand has all the info needed to construct the __________other half. If strands are separated, _____________ rules allow you to fill in the complementary bases. matching base-pairing

34. Figure 12–11 DNA Replication Section 12-2 Growth Replication fork DNA polymerase New strand Original strand DNA polymerase Nitrogenous bases Replication fork Original strand New strand Sites where strand separation and replication occur are called _____________ replication forks

35. REPLICATION STEPS 1.Enzymes “unzip” molecule by breaking _______________ that hold the strands together and unwind it. 2. _______________ joins nucleotides using original strand as template and ______________for errors. 3. Copying happens in ________ directions along the two strands & in __________ places at once. Hydrogen bonds DNA polymerase spell checks opposite multiple

36. See a video clip about DNA REPLICATION (12B)12B REPLICATION ANIMATION

37. ACTIVITY BE A DNA MOLECULEDNA MOLECULE

38. RNA and PROTEIN SYNTHESIS 12-3 © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

39. RNA- the Other Nucleic Acid Also made of ___________ Sugar is _______ instead of deoxyribose. RNA is _________ stranded Contains _________ instead of thymine. NUCLEOTIDES RIBOSE SINGLE URACIL http://images2.clinicaltools.com/images/gene/dna_versus_rna_reversed.jpg

40. 3 KINDS OF RNA HELP WITH INFO TRANSFER FOR PROTEIN SYNTHESIS _________________RNA (rRNA) Combines with proteins to form ribosomes _________________RNA (tRNA) Matches m-RNA codon to add correct amino acids during protein synthesis _________________RNA (mRNA) carries code from DNA to ribosomes rRNA and t-RNA images from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved mRNA image from http://wps.prenhall.com/wps/media/tmp/labeling/1140654_dyn.gif RIBOSOMAL TRANSFER MESSENGER

41. RNA DNA RNA polymerase Figure 12–14 Transcription Section 12-3 Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only) Enzyme called _____________________ separates strands, then uses one strand as a template to assemble an RNA copy. RNA POLYMERASE

42. How does RNA POLYMERASE know where a gene starts and stops? Enzyme binds to places with specific DNA sequences called _______________. PROMOTERS tell _________________ where to start. Signals at the end of the gene code cause transcription to _____. PROMOTERS http://images2.clinicaltools.com/images/gene/dna_versus_rna_reversed.jpg RNA POLYMERASE stop

43. See a video clip about TRANSCRIPTION (12C)12C Video 3 See another transcription animation Transcription animation

44. ACTIVITY TRANSCRIPTION of DNA

45. RNA’s require EDITING before use Image by Riedell

46. WHY WASTE IT? Why spend energy making a large RNA and then throw parts away? May allow same gene to be used in different ways in different kinds of cells. May have a role in evolution… allows small changes in genes to have a big effect.

47. MASTER PLAN DNA stays safe in nucleus TRANSCRIPTION (DNA → RNA) & PROCESSING takes place in nucleus TRANSLATION (RNA → proteins) takes place on ribosomes in cytoplasm © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved “Blueprints” of master plan are carried to building site http://www.home-improvement-resource.com/images/architect.jpg

48. HOW CAN JUST 4 BASES GIVE DIRECTIONS TO MAKE 20 AMINO ACIDS? Message is read in groups of 3 = _________ UCGCACGGU UCG-CAC-GGU CODON Codons represent different amino acids Serine- Histidine- Glycine

49. The m-RNA Code Section 12-3 64 possible codons Some amino acids have more than one codon. START= _______ 3 codons for _____ AUG STOP

50. ___________ on tRNA matches up with ________ on mRNA Images modified from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ANTICODON CODON EACH tRNA carries only one kind of _____________ amino acid

51. Figure 12–18 Translation Section 12-3

52. Figure 12–18 Translation (continued) Section 12-3

53. See a video clip about PROTEIN SYNTHESIS (12D)12D Video 4 SEE ANOTHER Translation Animation TRANSLATION VIDEO TRANSLATION VIDEO (Choose Large video)

54. DNA → DNA ____________ DNA → RNA ____________ RNA → Protein ___________ REPLICATION TRANSCRIPTION TRANSLATION

55. CENTRAL DOGMA OF BIOLOGY How is information passed? _____ → ______ → ________ DNARNA PROTEIN Then the protein “DOES SOMETHING” that shows up as a ________ trait

56. GENES & PROTEINS Proteins are the connection between the gene code in the DNA and how that gene is expressed. A gene that codes for an enzyme (protein) to make a pigment can control the color of a flower. A gene that codes for an enzyme (protein) adds carbohydrates to glycoproteins to produce your blood type. Enzymes catalyze and regulate chemical reactions so proteins build and operate all cell components. Mendel/flower images from: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC.html Blood cell by Riedell

57. fromtoto make up Concept Map Section 12-3 also calledwhich functions toalso called which functions to can be

58. fromtoto make up Concept Map Section 12-3 also calledwhich functions toalso called which functions to can be RNA Messenger RNA Ribosomal RNA Transfer RNA mRNACarry instructions rRNA Combine with proteins tRNA Bring amino acids to ribosome DNARibosomeRibosomes

59. MUTATIONS 12-4

60. _______________ are changes in the genetic material. MUTATIONS mistakes REMEMBER! Mutations can happen when cells make _____________ in copying their own DNA or be caused by _______________ or ___________ in the enviroment. radiation chemicals

61. Mutations that produce changes in a single gene = ______________________ Mutations that produce changes in whole chromosomes = _____________________ KINDS OF MUTATIONS GENE MUTATIONS CHROMOSOMAL MUTATIONS

62. Mutations involving ________________ ____________ = __________________ because they occur at a single point in the DNA sequence. TYPES OF POINT MUTATIONS:POINT MUTATIONS _____________________ GENE MUTATIONS One or a few nucleotidesPoint mutation substitutions deletions insertions

63. SUBSTITUTION Changes one base for another A T T C G A G C T A T T C T A G C T

64. SICKLE CELL ANEMIA CAUSE: (autosomal recessive) A changed to T (glu to val) gene on chromosome #11 that codes for part of hemoglobin protein (carries oxygen in blood)

65. DELETION ________________________________________ Piece of DNA code for one gene is lost Image from: http://www.biology-online.org/2/8_mutations.htm

66. Duchenne Muscular Dystrophy CAUSE: (X linked recessive) DELETION in gene that codes for a muscle protein

67. INSERTION Piece of DNA is copied too many times Image from: http://www.biology-online.org/2/8_mutations.htm

68. Substitutions usually affect no more than a single ____________, but deletions and insertions can have a more dramatic effect. GENE MUTATIONS Amino acid IMAGE FROM BIOLOGY by Miller and Levine; Prentice Hall Publishing©2006

69. FRAME SHIFT MUTATIONS Change multiple bases in code thefatcatatetherat ____________________ INSERTION thefatcatateateateatetherat DELETION thefatcatatetherat the fat cat ate ate ate ate the rat the fat ata tet her at

70. Frame shift mutations change every ___________ in the ___________ that follows the shift. Frame shifts can alter a protein so much it is unable to _____________ FRAME SHIFTS Amino acid protein function

71. Location of the shift is important! AT BEGINNING the fat cat ate the rat AT END the fat cat ate the rat the fac ata tet her at the fat cat ate thr at MUTATIONS AT _____________OF GENE DAMAGE __________ OF THE CODE! BEGINNING MORE

72. Mutations involving changes in the _____________ or ______________ of whole chromosomes TYPES OF CHROMOSOMAL MUTATIONS: _____________________ CHROMOSOMAL MUTATIONS Numberstructure deletions duplications inversions translocations See a Video (deletions & duplications See a Video (inversions & translocations

73. DELETION ________________________________________ Piece of chromosome is lost Image from: http://www.biology-online.org/2/8_mutations.htm

74. DUPLICATION Piece of DNA is copied too many times ________________________________________________ Image from: http://www.biology-online.org/2/8_mutations.htm

75. HUNTINGTON’S Degenerative brain disorder Symptoms appear age 30-40 Lose ability to walk, think, talk, reason Cause = ADDITION of extra CAG repeats

76. INVERSION Segment flips and reads backwards Image from: http://www.biology-online.org/2/8_mutations.htm

77. TRANSLOCATION Segment breaks off and joins a different non-homologous chromosome Image from: http://www.biology-online.org/2/8_mutations.htm

78. Most mutations are ____________ meaning they have little or no effect on gene ____________. Mutations that cause ________________ are usually ____________ Harmful mutations are associated with many ________________ and can cause ____________ MUTATIONS neutral function defective proteins HARMFUL genetic disorders cancer

79. Can help an organism _________________ Provide _________ in population for ____________ to act upon Mutations are also a source of _________________ and can be _____________ MUTATIONS Genetic variability beneficial Survive and reproduce variation natural selection MORE ON THIS 2 nd SEMESTER!

80. Condition in which an organism has extra sets of chromosomes = _______________ __________ in humans, but beneficial in some ___________. Triploid (___) or tetraploid (___) plants are often ________________ than diploid plants. POLYPLOIDY LETHAL 3N plants 4N larger and stronger

81. GENE REGULATION 12-5 http://www.awesomebackgrounds.com/s-energy-and-power.htm

82. Only a fraction of genes in a cell are expressed (made into RNA) at any given time. How does the cell decide which will be turned on and which will stay “silent”? You already know about _____________ regions that show RNA polymerase where to start. There are other ______________________ that control whether a gene is ON or OFF. PROMOTER REGULATORY SITES

83. Regulatory sites Promoter (RNA polymerase binding site) Start transcription DNA strand Stop transcription Typical Gene Structure Section 12-5

84. E. Coli lac operon Group of genes that operate together are called an ________________ OPERON http://www.life.uiuc.edu/bio100/lectures/s97lects/16GeneControl/lac_operon_ind.GIF Genes code for enzymes needed to digest lactose sugar. Only needed if glucose is not available See a MOVIE choose animation/narrated

85. Most of time glucose is available so lac operon is turned _____ by a ____________ molecule that sits on a regulatory site next to the promoter called the ___________ REPRESSOR OPERATOR OFF

86. Cells need to get rid of the repressor and turn _____the lac genes to digest lactose instead. The presence of lactose causes a change in the ____________ molecule so so it can’t bind the operator site. REPRESSOR ON What if there’s NO GLUCOSE? Image modified from: http://www.life.uiuc.edu/bio100/lectures/s97lects/16GeneControl/lac_operon_ind.GIF

87. Cells turn genes ON & OFF as needed Many genes are regulated by _____________ proteins that keep them turned off until needed. Others use proteins that speed up _______________ or affect ___________________ REPRESSOR transcription protein synthesis

88. EUKARYOTES are more COMPLEX Additional regulatory sequences: 1. ___________ regions upstream from promoters bind many different regulatory proteins 2. __________ (TATATA or TATAAA) helps position RNA POLYMERASE TATA box Image by Riedell ENHANCER

89. DEVELOPMENT & DIFFERENTIATION Gene regulation is also important in shaping way organisms develop How does a zygote become a multi-cellular organism? How does it know what kind of cell to be?

90. DEVELOPMENT & DIFFERENTIATION Cells ________________ by turning different genes on and off. BUT… How does a cell know where it is in the body? and what genes it should turn on? and when? DIFFERENTIATE http://www.ncu.edu.tw/~ls/graph/faculty_pictures/whole_time/SLC/SLC_lab-1.jpg

91. In the 1980s, researchers discovered a series of genes in fruit flies called ___________ These genes control the organization of the developing embryo and tell parts where to grow and when. Mutations to Hox genes can cause a leg to grow where an antenna should sprout. http://evolution.berkeley.edu/evosite/history/hox.shtml Hox genes

92. Since that time, HOX genes with almost identical sequences have been found in a variety of organisms including ____________ HUMANS © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

93. HOX GENES Similar genes controlling the eyes of insects and our own eyes have also been discovered. Our version of the gene can be inserted in a fly and still trigger the building of an insect eye! http://evolution.berkeley.edu/evosite/history/hox.shtml

94. SO WHAT? The similarities between HOX gene sequences in very different organisms and the ability of these genes to trade places and still function in different species suggests that these organisms __________________________ share a common ancestor