1. MMP’s and Their Role in the Wound Victor L. Sylvia, Ph.D. Associate Professor Department of Orthopaedics University of Texas Health Science Center 7703 Floyd Curl Drive San Antonio, TX 78220

2. http://www.med.ufl.edu/IDP/Core/Section%205/IDP%20Grand%20Rounds%20Tissue%20Engineering%202005%20version%203%20compressed.pdf

6. MMPs and Growth Factors Pathological dysregulation of MMP activity results in excessive breakdown of the extracellular matrix (ECM) and loss of growth factors, involved in normal healing, that are stored in the ECM. Growth factors such as PDGF-BB, TGF-β and VEGF are found in decreased amounts in chronic wounds, presumably due to the increase in MMPs (Loot et al., 2002; Lerman et al., 2003; Robson et al., 2004).

8. http://www.med.ufl.edu/IDP/Core/Section%205/IDP%20Grand%20Rounds%20Tissue%20Engineering%202005%20version%203%20compressed.pdf

10. Repair

11. http://www.med.ufl.edu/IDP/Core/Section%205/IDP%20Grand%20Rounds%20Tissue%20Engineering%202005%20version%203%20compressed.pdf

16. MMPSynonymsEC ClassificationLatent M.W. (kDa)Active M.W. (kDa)Substrates MMP-1Interstitial Collagenase3.4.24.75545Fibrillar collagens, casein, gelatin, proteoglycan MMP-2Gelatinase A, 72 kDa Type IV Collagenase 3.4.24.247266Denatured collagens, collagens IV, V, VII, X, gelatin MMP-3Stromelysin-13.4.24.175745Proteoglycan, collagens X, XI, procollagens, gelatin, laminin, collagenase, gelatinase B MMP-7Matrilysin ; PUMP3.4.24.232819As stromelysin 1, elastin MMP-8Neutrophil collagenase3.4.24.347558Fibrillar collagens, gelatin, proteoglycan MMP-9Gelatinase B, 92 kDa Type IV Collagenase 3.4.24.359286Denatured collagens, collagens IV, V, VII, X MMP-10Stromelysin 23.4.24.225744As Stromelysin 1 MMP-11Stromelysin 3 6247Unknown MMP-12Macrophage metalloelastase 3.4.24.655422Elastin, gelatin, collagen IV, fibronectin, laminin, vitronectin, proteoglycan MMP-13Collagenase 3--6648Fibrillar collagens, gelatin, aggrecan Matrix Metalloproteinases and their Substrates

18. http://www.med.ufl.edu/IDP/Core/Section%205/IDP%20Grand%20Rounds%20Tissue%20Engineering%202005%20version%203%20compressed.pdf

19. Remodeling

21. http://www.med.ufl.edu/IDP/Core/Section%205/IDP%20Grand%20Rounds%20Tissue%20Engineering%202005%20version%203%20compressed.pdf

27. Effect of Nitric Oxide Donor SNAP on Human Skin Fibroblasts

28. Effect of Nitric Oxide Donors on MMP-9 Expression

29. Effect of Nitric Oxide Donors on MMP-8 Expression

30. Effect of Nitric Oxide Donors on Wound Healing

31. Questions?