1. P1 1 NACO Lab Technician Training Programme

2. P2 2 What do you know about HIV/AIDS? Do you feel apprehensive to be involved in HIV Testing? What are your expectations from this course? Introduction

3. P3 3 What do LTs want to achieve? Learn about all aspects HIV/AIDS Understand all the various issues and processes/procedures of HIV testing at ICTCs Perform laboratory tests in accordance with NACOs HIV testing strategies and testing guidelines Understand the interpretation of results Ensure standardized and quality testing all across in order to provide accurate, reliable and reproducible test results and meet the requirements of the client

4. P4 4 Enhance the theoretical and practical skills of LTs with hands on practical sessions and exercises What do we want to achieve? Performance of Rapid HIV tests Performance of ELISA assays Exercises on: -collection, storage and transport of samples -interpretation of results, - maintenance of equipment, -practice of quality assurance, -making of positive control for day to day QC -inventory management -quality control Exercise on NACO HIV testing strategies

5. P5 6 What do we want to achieve? Reduce stigma within the healthcare personnel - address anxieties and misconceptions to increase willingness to care for HIV positive people Prevent occupational exposure to blood borne pathogens by training the healthcare providers in Biosafety, standard work precautions and PEP Assure and inform about PEP and its timely use in case of accidental exposure to minimize transmission of HIV

6. P6 7 Content of Training Module 1: Introduction Module 2: Virology and pathogenesis of HIV infection Module 3: Detection of HIV infection, HIV testing protocols and Strategies Module 4: Biosafety in the laboratory and standard work precautions Module 5: Process and flow of the Client at ICTC/PPTCT for HIV counseling and testing Module 6: Collection, transport and storage of blood sample Module 7: Elements/components of Total Quality Management/Quality System Essentials Module 8: Introduction to HIV testing

7. P7 8 Content of Training Module 9: Inventory management Module 10: Document control and various documents Module 11: Common errors, causes and trouble shooting Module 12: External quality assessment Module 13: Equipment maintenance and calibration Module 14: Performance and characteristics of ELISA, validation, use of Levey Jenning chart Module 15: Performance and characteristics of HIV rapid tests Module 16: Preparation of positive control, calculation of range for validation of ELISA for day to day QC

8. P8 9 Reference material to be provided Program agenda Hand outs of slides, practical exercises, examples of documents-Temperature log, inventory management, SOP NACO lab technician manual NACO website: www.nacoonline.org