1. Jan-Willem de Gier Center for Biomembrane Research, Stockholm, Sweden Green Light for Membrane Proteins

2. David Drew et al. Nat Methods (2006) 4:303-13

3. Monitoring overexpression of a YedZ-GFP fusion using in-gel and whole-cell fluorescence David Drew et al. Nat Methods (2006) 4:303-13

4. From detergent screen to recovered membrane protein David Drew et al. Nat Methods (2006) 4:303-13

5. Recovered membrane proteins can be used for functional and structural studies David Drew et al. Protein Sci (2005) 14: 2011-2017

6. Simon Newstead et al. PNAS (2007) 104(35):13936-41 GFP-based pipeline in yeast

7. Biogenesis of membrane proteins in E. coli Samuel Wagner et al. (2006) Trends Biotechnol 24: 364-371 membrane proteins secretory proteins

8. E. coli BL21(DE3)/pET overexpression system Merck Novagen, pET manual

9. Experimental setup (1) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

10. Experimental setup (2) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

11. Consequences of membrane protein overexpression in E. coli (1) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

12. Consequences of membrane protein overexpression in E. coli (2) Samuel Wagner et al. (2007) Mol Cell Proteomics (9) 1527-50.

13. Characterization of C41(DE3) and C43(DE3), also known as the Walker strains

14. Differences in overexpression kinetics? Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

15. Differences in overexpression kinetics

16. Effect of the addition of IPTG Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

17. Mutations in the promoter governing T7RNAP expression in C41(DE3) and C43(DE3) Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

18. Mimicking the effect of the mutations in the promoters with T7 lysozyme Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

19. Mimicking the effect of the mutations in the promoters with T7 lysozyme Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

20. Characterization of Lemo21(DE3) Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

21. Lemo21(DE3) is an ’all in one’ platform for membrane protein overexpression Samuel Wagner et al. (2008) PNAS, 105 (38):14371-6.

23. Funding -Swedish Research Council (VR) -Wallenberg Foundation -Swedish Foundation for Strategic Research (SSF) -National Institutes of Health (NIH) www.xbranebio.com www.cbr.su.se