1. Lecture 10 – Second Messengers IV – Niric Oxide, cGMP, and ROS Signalling BIOL 5190/6190 Cellular & Molecular Singal Transduction Prepared by Bob Locy Last modified -13F

2. CSB Fig 02:01 Cell signalling pathways CSB Fig 02:01 Cell signalling pathways Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012

3. Transducers Second Messengers II 1.Nitric Oxide cyclic GMP Signaling cyclic GMP Signaling 2.Redox signaling 3.ROS signaling

4. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:45 NO and cyclic GMP signalling CSB Fig 2:45 NO and cyclic GMP signalling

5. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:46 NO synthase mechanism CSB Fig 2:46 NO synthase mechanism

6. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:47 eNOS activation CSB Fig 2:47 eNOS activation

7. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:48 Summary of redox signalling CSB Fig 2:48 Summary of redox signalling

8. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:49 Reactive oxygen species (ROS) CSB Fig 2:49 Reactive oxygen species (ROS)

9. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:50 Sites of ROS formation CSB Fig 2:50 Sites of ROS formation

10. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:51 Plasma membrane ROS formation CSB Fig 2:51 Plasma membrane ROS formation

11. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:52 GSH/GSSG couple CSB Fig 2:52 GSH/GSSG couple

12. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:53 H2O2 metabolism CSB Fig 2:53 H2O2 metabolism

13. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:54 Peroxiredoxin catalytic cycles CSB Fig 2:54 Peroxiredoxin catalytic cycles

14. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:55 Reversible & irreversible ROS oxidations CSB Fig 2:55 Reversible & irreversible ROS oxidations

15. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:56 ROS formation and action CSB Fig 2:56 ROS formation and action

16. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:57 Recovery of protein oxidation CSB Fig 2:57 Recovery of protein oxidation

17. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:58 Thimerosal-induced Ca2+ signalling CSB Fig 2:58 Thimerosal-induced Ca2+ signalling

18. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Module 2:59 Figure ROS effects on Ca2+ signalling Module 2:59 Figure ROS effects on Ca2+ signalling

19. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:60 ROS microdomains CSB Fig 2:60 ROS microdomains

20. Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 Cell Signalling Biology - Michael J. Berridge - www.cellsignallingbiology.org - 2012 CSB Fig 2:61 ROS microdomains CSB Fig 2:61 ROS microdomains