1. Tuesday, March 6, 2012  Open to entry- Protein Networks from yesterday Homework: 1)Cell Transport retakes in tutorial- LAST DAY 2)Quiz on Protein Pathways/Protein Structure- FRI 3)Serotonin Pathway worksheet- due on Block Day

2. “Coat Color in Labrador Retrievers” CocoaMidnight

3. Guiding Question: “How does variation exist between organisms?” Read: “Coat Colors in Labrador Retrievers” to help you answer this question.

4. Vocab to know for reading. Pigment = A substance, such as chlorophyll or melanin, that produces a characteristic color in plant or animal tissue. Skin and Hair pigments are produced in cells called melanocytes. Synthesize = to put together.

5. Labrador (dog) pigmentation Tyrosine Dopaquinone Brown pigment Black pigment Yellow pigment tyrosinase TRP-2 TRP-1 Boxes = metabolites Arrows = enzymes that change the metabolites What will be the dog’s coat color? BLACK

6. Tyrosine Dopaquinone Brown pigment Black pigment Yellow pigment tyrosinase TRP-2 TRP-1 What would happen if the enzyme TRP – 1 was missing? Black pigment What color is the dog’s coat?Brown Boxes = metabolites Arrows = enzymes that change the metabolites

7. Tyrosine Dopaquinone Brown pigment Black pigment Yellow pigment tyrosinase TRP-2 TRP-1 So what would happen if the enzymes TRP – 1 and TRP-2 were both missing? Black pigment What color is the dog’s coat?Yellow Brown pigment Boxes = metabolites Arrows = enzymes that change the metabolites

8. Make an entry: Entry 17: Protein Pathways Practice- 3/6/12 See attached

9. Pigment Metabolic Network for the Imaginary Bioflower Blue Flower Purple Flower Where is the variation in these flowers? Blue and Purple Flowers (Color)

10. Bioflowers A colorless starting molecule is converted by enzyme X to blue pigment. Next, enzyme Y converts the blue pigment to purple pigment. 1a.Diagram of the pathway. (include a key) Colorless Compound Blue PigmentPurple Pigment XY

11. 1b.Give an explanation for a blue flower.  Enzyme Y not available or not working

12. “Roundbuds”

13. Roundbuds In another type of wildflower, the roundbud, red pigment is synthesized from a white precursor by enzyme Q. 1.Draw the enzyme pathway for the roundbud. It was believed that all roundbuds were red until a knowledgeable, observant teenager discovered a meadow full of white-flowered roundbuds. 2. Using your knowledge of the synthesis pathway for red pigment, give an explanation for the white roundbud variety.

14. White Precursor Red Pigment Q 2a. Draw Enzyme Pathway In another type of wildflower, the roundbud, red pigment is synthesized from a white precursor by enzyme Q.

15. 2b. It was believed that all roundbuds were red until a knowledgeable, observant teenager discovered a meadow full of white-flowered roundbuds.

16. Enzyme Q is not working- therefore no conversion to red pigment. 2b. Using your knowledge of the synthesis pathway for red pigment, give an explanation for the white roundbud variety. White PrecusorRed Pigment Q

17. YELLOW PIGMENT IS CONVERTED BY ENZYME Z INTO BROWN PIGMENT YELLOW CAN ALSO BE CONVERTED BY ENZYME W INTO RED PIGMENT BROWN PIGMENT IS CONVERTED BY ENZYME X INTO BLACK PIGMENT BROWN CAN ALSO BE CONVERTED BY ENZYME Y INTO ORANGE

18. YELLOW PIGMENT RED PIGMENT BLACK PIGMENT BROWN PIGMENT ORANGE PIGMENT Z W X Y Chupacabra Coat Color Yellow= no Z or W Brown= no X or Y, W RED= no Z

19. #3. Fancy flowers a. b.Red with white spots c.No spots, red only RED RED-WHITE SPOTS RED-BLUE SPOTS S T

20. ABC D EF 1 2 3 4 5 #4 a.1,2 b.A,B, C, D c.All except F d.1, 3

21. Make an entry- Entry 18: Human Metabolic Networks- 3/6/12 Substrate Enzyme  Product Add to last question on back: How is a MAO inhibitor used to treat depression?