1. Varian 900-LC Series iHPLC

2. What is the iHPLC? 2 models 920-LC and 940-LC
Compact, stylish, automated analytical and semi prep HPLC
Pre-plumbed and factory tested for the highest confidence
Easy to order, install and use
Single Ethernet cable connection
Focused on improved productivity through automation
Updated premium performance hardware
Autosampler, Fraction Collector and Scale up Module for semi-prep
Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical
Updated Integrated Instrument Software Interface
Eliminate repetition, modernise UI
Consistent
Easy to use with added functionality
Adds to modular range

3. Varian 920-LC Integrated Analytical HPLC

4. Varian 920-LC What Is It? Compact, stylish automated analytical HPLC
Pre-plumbed and factory tested for the highest confidence
Single cable communications
Virtually pulse-free dual piston pump
Does not require a pulse damper
State-of-the-art low pressure quaternary gradient module with built-in degasser
Autosampler with cooling option
Synchronised injection for outstanding retention time reproducibility
Compatible with 200 x 1.6 ml HPLC vials or 3 microplates

5. Varian 920-LC What Is It? Either dual l UV-Vis or PDA detector
Optional second detector - Fluorescence or RI
High precision (<0.15oC) dual column heater available
Low delay solvent pre-heater for improved heat transfer
Optional column switching and electric injection valve
NEW improved instrument interface on GalaxieTM software
External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.

6. Varian 920-LC Silver Bullets
Premium analytical pump performance and reliability
Superb Fast LC performance optimised for sub 3 um columns
Improved throughput without compromising robustness
High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength
Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler
Full system level computer controlled AIQ (validation)
Updated driver for enhanced user experience

7. 920-LC from Conventional to Fast HPLC
25cm x 4.6mm Pursuit XRS 5um 1.0ml/min
25cm x 4.6mm Pursuit XRS 5um 2.2ml/min
15cm x 4.6mm Pursuit XRS 5um 1.0ml/min
15cm x 4.6mm Pursuit XRS 5um 3.75ml/min
10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min
5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min
16 x Speed Enhancement
5cm x 2.0mm 2.4um 1.3ml/min

8. Varian 920-LC Fast LC PAHs Varian 920-LC 6 minute Analysis
Waters Acquity
7 Minute Analysis

9. Varian 920-LC Solvent Delivery System
Dual piston quaternary pump produces fast gradients – even at 200 ml/min
Virtually pulse-free pumping without pulse damper
Outstanding gradient compositional accuracy give retention time precision at the <1% level
Built-in vacuum degas maintains pump prime and enhanced injection reliability
Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns
Overlay of 10 chromatograms
10% steps at 1ml/min

10. Varian 920-LC Autosampler
Needle-in-flow path geometry for low carry-over (<0.01%)
Typical inj precision (< 0.3% at 10 ml)
Optimized flow paths for fast injection turn-around
Synchronised injection for best RT reproducibility
Programmable wash setting for low carry-over
Injection volumes from 1ul to 50mL
Cooled option available

11. Varian 920-LC Washout Performance
125 ug/ml Caffeine
Blank injection <0.01% carryover

12. Varian 920-LC Injection Synchronization

13. Sensitive UV-Vis Detectors
Typical Agilent VWD S/N ~ 25
Typical Varian 920-LC UV/Vis S/N > 36

14. Information Rich Diode Array Detection
1024 pixels provides full coverage from 190 – 950 nm
Full l spectra collection
Enhanced purity
Automatic peak identification with customised libraries
Greater confidence on compound identification
Choice of flowcells
4 mm path length semi-micro
9mm path length analytical
Front panel access
Lamps and flowcells
D2 and Vis lamps self aligned
Easily user maintained

15. Information Rich Diode Array Detection
Automatic peak identification with customised libraries
Use spectra as well as RT for compound ID
Greater confidence on compound identification
Peak search of unknown yields match for naphthalene

16. Varian 920-LC Fluorescence Detector
Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)
Time programmable l for increase sensitivity
Automatic l validation with built-in Hg lamp
Low 12 ml dead volume flowcell for improved chromatography

17. Varian 920-LC Refractive Index Detector
Based upon 356-LC RI detector
Low volume, flow efficient cell for superior peak shape
Stable, long life and high intensity LED
Minimises drift
Provides excellent detection limits
Enhanced life-time
Temperature controlled optics minimises baseline drift and provides outstanding detection limits
Complete automation thru Galaxie software

18. Universal HPLC detector
Varian 380-LC and 385-LC ELSD
Universal HPLC detector
Compatible with gradients
More sensitive than RI
NEW Glass Nebuliser for Improved Reproducibility
2 to 3 Times More Sensitive
40Hz Data Rate for Fast LC
Complete Galaxie compatibility
Program changes thru a run

19. Heated Column Compartment
Temperature range from ambient + 10oC up to 65oC
Excellent temperature precision +0.15oC
Ensures retention time reproducibility
Eliminates re-runs
Good temperature accuracy <1oC
Low delay volume pre-heater for efficient heat transfer to mobile phase
Rapid warm-up
Typically from ambient to 40oc in <15 minutes
Spacious - easily accommodates 2 x 250mm columns and 2 valves
Option with electrically actuated column switching valve and injection valve

20. Excellent Long term Stability

21. Quick Warmup - Outstanding Stability

22. Reproducibility of 50 Injections
Reproducibility <0.8% over 50 injections and 12 hours

23. Varian 920-LC Software Usability
User friendly GalaxieTM software
Simplified, modern, consistent Instrument interface
Modular 11 pages
920-LC 4 pages!!
One page real-time status and chromatogram
Advanced integration and calibration
New IMS capabilities
Automated instrument validation
Reduces downtime and operating costs
Fusion AE

24. Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC
Leak detector
Increases confidence with unattended automated runs
Single waste point
Ensures capture of all wastes
Easy to connect to waste vessel
Built-in Solvent bottle storage
Improved safety and convenience
Lower hight than modular systems

25. Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC
Clear front doors
Stylish
Provides access to autosampler without opening
Safety increased as lab staff shielded from solvent spray and leaks
Useful, convenient storage compartments
Column rack in door
Tool drawer
Operation log drawer

26. Varian 920-LC AIQ (Validation)
AIQ (Analytical Instrument Qualification)
Fully PC controlled IQ and OQ (not PQ)
Puts the Varian 920-LC at the forefront of regulated capabilities
Consistent, reproducible methodology
Includes pumps, autosampler, detectors, column heater
Chargeable option
Fully documented

27. 900 LC -Series AIQ
Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package.
Verifies the entire LC system by systematically running the following
Flow Rate Accuracy Test
Wavelength Detector Accuracy Test
Auto-sampler Injector Accuracy Test (also measures Detector Linearity)
Auto-sampler Injector Precision Test
Auto-sampler Injector Carryover Test
Proportioning Accuracy Test
Detector Noise Test
Detector Drift Test
Auto-sampler Vial Identification Accuracy Test
Proportioning Linearity
Compositional Ripple

28. Varian 920-LC Configurations
pump
inject
detector
Oven
Detector 2
option
Options
Isocratic
Dual piston pump 10 ml head
Auto
Dual l UV/Vis
Opt
FL, RI
ELSD
Gradient
Quat, Grad, Degas
ELSD opt
High Performance Gradient
Auto cooled
Development
PDA
Std
Research

29. Varian 920-LC Consumables
Excellent range of HPLC Columns providing industry leading resolution and robustness
Pursuit
Pursuit XRS
Polymeric Phases - PL
From 2mm to 2” ID Prepacked
Other Speciality Phases
NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC
2.8um Ultra is optimised for Speed
2.4um gives further enhancements in Resolution

30. 2.8um Vs 2.4 um – High Res or Extra Speed
2.4um 3mm x 5cm for RESOLUTION
2.8um 2mm x 5cm for speed

31. Questions??