1. Cell Structure Chapter 4

2. 2 Cell Theory 1.All organisms are composed of cells 2.Cells are the smallest living things 3.Cells arise only from pre-existing cells All cells today represent a continuous line of descent from the first living cells

3. 3 Cell size is limited Most cells are relatively small due reliance on diffusion of substances in and out of cells Rate of diffusion affected by –Surface area available –Temperature –Concentration gradient –Distance

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5. Surface area-to-volume ratio Organism made of many small cells has an advantage over an organism composed of fewer, larger cells As a cell’s size increases, its volume increases much more rapidly than its surface area Some cells overcome limitation by being long and skinny – like neurons 5

6. What do all cells do? Make more cells/DNA Make energy Make proteins Transport materials into and out of cell 6

7. What part of the cell does this? Make more cells  Energy  Proteins  Transport into and out of cells 7

8. 8 Basic structural similarities 1.Nucleoid or nucleus where DNA is located 2.Cytoplasm –Semifluid matrix of enzymes/organelles –Cytosol 3.Ribosomes –Synthesize proteins 4.Plasma membrane –Phospholipid bilayer

9. 9 Prokaryotic Cells (Simplest organisms) Lack a membrane-bound nucleus –DNA is present in the nucleoid (“cluster”) Enzymes Do contain ribosomes (not membrane- bound organelles) Cell membrane Cell wall outside of plasma membrane

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11. Two Domains of Prokaryotes Two domains of prokaryotes –Archaea Extremophiles –Bacteria “Bacteria” 11

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13. ProkaryotesArchaeCell WallBacteriaCell WallPeptidoglycan 13

14. Prokaryotic cell walls Archaea lack peptidoglycan Most bacterial cells are encased by a strong cell wall –composed of peptidoglycan –Cell walls of plants, fungi, and most protists different Protect the cell, maintain its shape, and prevent excessive uptake or loss of water Susceptibility of bacteria to antibiotics often depends on the structure of their cell walls 14

15. EUKARYOTIC CELLS 15

16. What do all cells do? Make more cells/DNA Make energy Make proteins Transport materials into and out of cell 16

17. BUT… Now these have specialized membrane- bound organelles! 17

18. 18 Eukaryotic Cells Possess a membrane-bound nucleus More complex than prokaryotic cells Compartmentalization –Achieved through use of membrane-bound organelles and endomembrane system Possess a cytoskeleton for support and to maintain cellular structure

19. What do all cells do? Make more cells/DNA  –Nucleus Make energy  –Mitochondria Make proteins  –Ribosomes Transport materials into and out of cell  –SER, RER, Golgi, Cell Membrane AND other specialized organelles 19

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22. 22 Nucleus Repository of the genetic information Most eukaryotic cells possess a single nucleus Nucleolus – region where ribosomal RNA synthesis takes place Nuclear envelope –2 phospholipid bilayers –Nuclear pores – control passage in and out In eukaryotes, the DNA is divided into multiple linear chromosomes –Chromatin is chromosomes plus protein

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24. Ribosomes * Ribosomes do NOT have a membrane Cell’s protein synthesis machinery Found in all cell types in all 3 domains Ribosomal RNA (rRNA)-protein complex Protein synthesis also requires messenger RNA (mRNA) and transfer RNA (tRNA) Ribosomes may be free in cytoplasm or associated with internal membranes 24

25. 25 Endomembrane System Series of membranes throughout the cytoplasm Divides cell into compartments where different cellular functions occur One of the fundamental distinctions between eukaryotes and prokaryotes

26. 26 Endoplasmic reticulum Rough endoplasmic reticulum (RER) –Attachment of ribosomes to the membrane gives a rough appearance –Synthesis of proteins to be secreted, sent to lysosomes or plasma membrane Smooth endoplasmic reticulum (SER) –Relatively few bound ribosomes –Variety of functions – synthesis, store Ca 2+, detoxification Ratio of RER to SER depends on cell’s function

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28. 28 Golgi apparatus Flattened stacks of interconnected membranes (Golgi bodies) Functions in packaging and distribution of molecules synthesized at one location and used at another within the cell or even outside of it Cis and trans faces Vesicles transport molecules to destination

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31. 31 Lysosomes Membrane-bounded digestive vesicles Arise from Golgi apparatus Enzymes catalyze breakdown of macromolecules Destroy cells or foreign matter that the cell has engulfed by phagocytosis Involved in apoptosis (cell death)

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33. Peroxisomes Variety of enzyme- bearing, membrane- enclosed vesicles Peroxisomes –Contain enzymes involved in the oxidation of fatty acids –H 2 O 2 produced as by- product – rendered harmless by catalase 33

34. 34 Vacuoles Membrane-bounded structures in plants Various functions depending on the cell type There are different types of vacuoles: –Central vacuole in plant cells –Contractile vacuole of some protists –Storage vacuoles

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36. 36 Mitochondria Found in all types of eukaryotic cells Bound by membranes –Outer membrane –Intermembrane space –Inner membrane has cristae –Matrix On the surface of the inner membrane, and also embedded within it, are proteins that carry out oxidative metabolism Have their own DNA

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38. 38 Chloroplasts Organelles present in cells of plants and some other eukaryotes Contain chlorophyll for photosynthesis Surrounded by 2 membranes Thylakoids are membranous sacs within the inner membrane –Grana are stacks of thylakoids Have their own DNA

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40. 40 Endosymbiosis Proposes that some of today’s eukaryotic organelles evolved by a symbiosis arising between two cells that were each free- living One cell, a prokaryote, was engulfed by and became part of another cell, which was the precursor of modern eukaryotes Mitochondria and chloroplasts

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42. 42 Cytoskeleton Network of protein fibers found in all eukaryotic cells –Supports the shape of the cell –Keeps organelles in fixed locations Dynamic system – constantly forming and disassembling

43. 43 3 types of fibers Microfilaments (actin filaments) –Two protein chains loosely twined together –Movements like contraction, crawling, “pinching” Microtubules –Largest of the cytoskeletal elements –Dimers of α- and β-tubulin subunits –Facilitate movement of cell and materials within cell Intermediate filaments –Between the size of actin filaments and microtubules –Very stable – usually not broken down

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45. Centrosomes Region surrounding centrioles in almost all animal cells Microtubule-organizing center –Can nucleate the assembly of microtubules Animal cells and most protists have centrioles – pair of organelles Plants and fungi lack centrioles 45

46. Eukaryotic cell walls –Plants, fungi, and many protists –Different from prokaryote –Plants and protists – cellulose –Fungi – chitin –Plants – primary and secondary cell walls 46

47. 47 Extracellular matrix (ECM) Animal cells lack cell walls Secrete an elaborate mixture of glycoproteins into the space around them Collagen may be abundant Form a protective layer over the cell surface Integrins link ECM to cell’s cytoskeleton –Influence cell behavior

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50. Cell-to-cell interactions Surface proteins give cells identity –Cells make contact, “read” each other, and react –Glycolipids – most tissue-specific cell surface markers –MHC proteins – recognition of “self” and “nonself” cells by the immune system 50

51. Cell connections 3 categories based on function 1.Tight junction –Connect the plasma membranes of adjacent cells in a sheet – no leakage 2.Anchoring junction –Mechanically attaches cytoskeletons of neighboring cells (desmosomes) 3.Communicating junction –Chemical or electrical signal passes directly from one cell to an adjacent one (gap junction, plasmodesmata) 51

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53. Cell Movement Prokaryotes and Eukaryotes can BOTH have flagella and cilia… 53 ProkaryotesEukaryotes Smaller and SimplerLarger and more complex Protein flagellinTubulin (9+ 2 microtubule arrangement) Rotary MotionBending movement Proton drivenATP driven

54. Prokaryotic Flagella 54

55. Eukaryotic Flagella 55