1. Research quality and collaboration Can Biobanks contribute? 12/05/2015 Loes Linsen

2. 2 What can I tell you? Operational manager of University Biobank Limburg (UBiLim) UBiLim: Collaboration of UH – ZOL – Jessa Director Prof. Dr. JL. Rummens Support activities of Limburg Clinical Research Program Strong expertise in biobank quality 2004: Participation in FP6 CCPRB > Good Biobanking Practices Embedded in ISO15189 accredited laboratory Implementation of CMI quality manual (audit +) Focus on biospecimen research Effect of preanalytics on research results Validation of processing procedures

3. Translational Research Gap No confirmation of research results Sample numbers Sample comparison Sample quality 3

4. 4 Why care about (sample) quality? May 2010 1500 glioblastoma samples for Cancer Genome Atlas 1 collection claimed for 12 000 samples of which ONLY 18 were acceptable Traceability Origin ELSI Fitness-for-Purpose!

5. Is it really that bad? 5 Serum spectra profiles (SELDI-TOF MS analysis) in healthy controls and PCa patients collected before 1996 (early) and after 1996 (late) (McLerran, D. et al. (2008) Clin Chem 54, 44–52.)

6. What can affect sample quality? 6

7. Processing delay & temperature: tissue 7 (Spruessel, A. et al. (2004) Biotechniques 36, 1030-1037.)

8. Processing delay & temperature: tissue 8 (Spruessel, A. et al. (2004) Biotechniques 36, 1030-1037.)

9. Processing delay & temperature: blood 9 (Clark, S. et al. (2003) Int J Epidemiol 32, 125–130.)

10. How to control? Impossible! Minimize damage with good study setup AND Track typical preanalytical factors: Standard PREanalytical Code (SPREC) (fluid – tissue) 7 item code sample and primary container type periods of cold and warm ischemia subsequent handling steps including speed and temperature of centrifugation and final storage temperature PL1-HEP-B-D-N-A-D Validate code to research needs 10

11. Does it work? 11 PL1-HEP-B-D-N-A-D PL1-HEP-D-D-N-A-D PL1-HEP-F-D-N-A-D

12. So why do you need a biobank? SPREC: easy to implement; cumbersome to apply manually Biobank Information System > default Not only preanalytics affect sample quality! Number of freeze/thaw cycles Storage/fixation duration Sample processing procedures ELSI Linked data (verification) … Biobank has expertise and systems in place to cover full process 12

13. 13 Why care about the biobank (quality)? © JH di Donato, 2011

14. Why care about the biobank quality? Sample (& data) quality is assured & garantueed in long term Within biobank Across biobanks in a network Flemish Virtual Biobank (CMI) Belgian Virtual Tumorbank (BVT) Trusted party for your samples 14

15. Do biobanks contribute to scientifc collaboration? Number of samples needed for high impact publications Omics techniques Rare diseases Confirmation of results (robustness) Sample quality of collaborators outside biobank? Built-in trust 15

16. Do biobanks stimulate collaboration? Joined interest in using samples with added value Specific data present for “all” samples Enrichment by feedback/availability of results In silico research on samples/data collected by others 16

17. 17 Conclusion? Biobanks are key facilitators for translational research Added value of biobanks Access to a magnitude of samples Not just a collection of freezers! Garbage In is Garbage Out (GIGO) Service provider FOR YOU Expertise, know how & know why Study improvement

18. Research quality and collaboration Can Biobanks contribute?

19. Acknowledgements Operational Group Prof. Dr. JL Rummens Prof. Dr. P Stinissen Prof. Dr. V Somers Prof. Dr. J Penders Many others in biobank endavours Funding: Biobank Team T Vanbinst C Motmans E Martens I Rutten K Ulenaers 19