1. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Chapter 27 Prokaryotes

2. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Overview: They’re (Almost) Everywhere! Most prokaryotes are microscopic, but what they lack in size they make up for in numbers There are more in a handful of fertile soil than the number of people who ever lived Prokaryotes thrive almost everywhere, including places too acidic, too salty, too cold, or too hot for most other organisms They have an astonishing genetic diversity

3. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings WORD ROOTS - GEN:EXO-: -OID:HALO-: AN-:MUTU--: ANTI-:-PHILOS: BI-:HALO-: CHEMO-:PHOTO-: ENDO-:SYM-:

4. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 27.1: Structural, functional, and genetic adaptations contribute to prokaryotic success Most prokaryotes are unicellular, although some species form colonies. Prokaryotic cells have a variety of shapes. The three most common shapes 1.Spheres (cocci) 2. Rods (bacilli) 3. Spirals (spirilla)

5. LE 27-2 Spherical (cocci) Rod-shaped (bacilli) Spiral 5 µm2 µm1 µm

6. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Cell-Surface Structures An important feature of nearly all prokaryotic cells is their cell wall, which maintains cell shape, provides physical protection, and prevents the cell from bursting in a hypotonic environment Using the Gram stain, scientists classify many bacterial species into groups based on cell wall composition, Gram-positive and Gram-negative

7. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings GRAM POSITIVE AND GRAM NEGATIVE GRAM POSITIVE: These bacteria have simpler walls with a relatively large amount peptidoglycan. GRAM NEGATIVE: These bacteria have less peptidoglycan and are structurally more complex, with an outer membrane that contains lipopolysaccharides, which are carbohydrates bonded to lipids.

8. Book Page 535 Draw and label in Notes each Gram Bacteria: Pepridoglycan layer Cell wall Protein Gram- positive bacteria Gram-positive Gram-negative Gram- negative bacteria Pepridoglycan layer Cell wall Plasma membrane Lipopolysaccharide Plasma membrane Protein Outer membrane 20 µm

9. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The cell wall of many prokaryotes is covered by a capsule, a sticky layer of polysaccharide or protein called a capsule.

10. LE 27-4 Capsule 200 nm

11. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Some prokaryotes have fimbriae and pili, which allow them to stick to their substrate or other individuals in a colony

12. LE 27-5 Fimbriae 200 nm

13. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Motility Most motile bacteria propel themselves by flagella that are structurally and functionally different from eukaryotic flagella In a heterogeneous environment, many bacteria exhibit taxis, the ability to move toward or away from certain stimuli

14. LE 27-6 Flagellum Filament Cell wall Hook Basal apparatus Plasma membrane 50 nm

15. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Internal and Genomic Organization Prokaryotic cells usually lack complex compartmentalization. Some prokaryotes do have specialized membranes that perform metabolic functions

16. LE 27-7 Thylakoid membranes Respiratory membrane Photosynthetic prokaryote Aerobic prokaryote 0.2 µm 1 µm

17. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The typical prokaryotic genome is a ring of DNA that is not surrounded by a membrane and that is located in a nucleoid region

18. LE 27-8 Chromosome 1 µm

19. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Some species of bacteria also have smaller rings of DNA called plasmids

20. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Reproduction and Adaptation Prokaryotes reproduce quickly by binary fission and can divide every 1–3 hours Many prokaryotes form endospores, which can remain viable in harsh conditions for centuries

21. LE 27-9 Endospore 0.3 µm

22. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Rapid reproduction and horizontal gene transfer facilitate the evolution of prokaryotes in changing environments

23. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings CLASS START PROPERTYDESCRIPTION CELL SHAPE CELL SIZE CELL SURFACE MOTILITY INTERNAL MEMBRANES GENOMES REPRODUCTION AND GROWTH

24. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PROPERTYDESCRIPTION CELL SHAPE ROD (BACILLUS), ROUND OR SPHERE (COCCI), SPIRAL (SPIRILLA) CELL SIZE 1-5 MICROMETERS IN DIAMETER CELL SURFACE CELL WALL MADE OF PEPTIDOGLYCAN; GRAM NEG BACTERIA ALSO HAVE OUTER LIPOPOLYSACHARIDE MEMBRANE; ARCHAEA LACK PEPTIDOGLYCAN; STICKY CAPSULE FOR ADHERENCE; FIMBRAE AND PILI FOR ATTATCHMENT OR CONJUGATION MOTILITY FLAGELLA; MAY SHOW TAXIS TO STIMULI INTERNAL MEMBRANES INFOLDINGS OF PLASMA MEMBRANE MAY BE USED IN METABOLIC FUNCTIONS GENOMES CIRCULAR DNA WITH LITTLE ASSOCIATED PROTEINS IN NUCLEOID REGION; MAY HAVE PLASMIDS WITH ANTIBIOTIC RESISTANCE AND OTHER GENES REPRODUCTION AND GROWTH BINARY FISSION; RAPID POPULATION GROWTH; SOME HORIZONTAL GENE TRANSFER; RAPID ADAPTIVE EVOLUTION

25. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 27.2: A great diversity of nutritional and metabolic adaptations have evolved in prokaryotes All four models of nutrition are found among prokaryotes: 1.Photoautotrophy 2.Chemoautotrophy 3.Photoheterotrophy 4.Chemoheterotrophy

26. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings MODE OF NUTRITION ENERGY SOURCE CARBON SOURCE TYPES OF ORGANISMS EXAMPLES PHOTO AUTOTROPH LIGHTCO2PHOTOSYNTHE TIC PROKARYOT; PLANTS; CERTAIN PROTISTS CYANOBACTERIA, ALGAE CHEMOAUTO TROPH INORGANIC CHEMICALS CO2CERTAIN PROKARYOTE SULFOLOBUS PHOTOHETER OTROPH LIGHTORGANIC CMPND CERTAIN PROKARYOTE RHODOBACTERIA, CHLOROFLEXUS CHEMOHETER OTROPH ORGANIC COMPOUND ORGANIC CMPND MANY PROKARYOTE AND PROTISTS, FUNGI, ANIMALS, SOME PLANTS CLOSTRIDUM

27. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Metabolic Relationships to Oxygen Prokaryotic metabolism varies with respect to oxygen: – Obligate aerobes require oxygen – Facultative anaerobes can survive with or without oxygen – Obligate anaerobes are poisoned by oxygen

28. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Nitrogen Metabolism Prokaryotes can metabolize nitrogen in a variety of ways In nitrogen fixation, some prokaryotes convert atmospheric nitrogen to ammonia

29. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Metabolic Cooperation Cooperation between prokaryotes allows them to use environmental resources they could not use as individual cells In the cyanobacterium Anabaena, photosynthetic cells and nitrogen-fixing cells exchange metabolic products

30. LE 27-10 Heterocyte Photosynthetic cells 20 µm

31. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In some prokaryotic species, metabolic cooperation occurs in surface-coating colonies called biofilms

32. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings

33. Concept 27.3: Molecular systematics is illuminating prokaryotic phylogeny Until the late 20th century, systematists based prokaryotic taxonomy on phenotypic criteria Applying molecular systematics to the investigation of prokaryotic phylogeny has produced dramatic results

34. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Lessons from Molecular Systematics Molecular systematics is leading to a phylogenetic classification of prokaryotes It allows systematists to identify major new clades

35. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A tentative phylogeny of some of the major taxa of prokaryotes based on molecular systematics

36. LE 27-12 Domain Bacteria Domain Archaea Domain Eukarya Universal ancestor Proteobacteria Alpha Beta Gamma Delta Epsilon Chlamydias Spirochetes CyanobacteriaGram-positive bacteria KorarchaeotesEuryarchaeotesCrenarcaeotesNanoarchaeotes Eukaryotes

37. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Bacteria Diverse nutritional types are scattered among the major groups of bacteria The two largest groups are the proteobacteria and the Gram-positive bacteria

38. LE 27-13 PROTEOBACTERIA Subgroup: Alpha Proteobacteria Rhizobium (arrows) 2.5 µm Subgroup: Beta Proteobacteria Nitrosomonas 1 µm Subgroup: Gamma Proteobacteria Chromatium 0.5 µm Subgroup: Delta Proteobacteria Chrondromyces crocatus 10 µm 2 µm Subgroup: Epsilon Proteobacteria Heliocobacter pylori 5 µm Bdellovibrio bacteriophorus CYANOBACTERIA Oscillatoria 50 µm 5 µm 1 µm GRAM-POSITIVE BACTERIA Streptomyces Mycoplasmas covering a human fibroblast cell 5 µm SPIROCHETES Leptospira 2.5 µm CHLAMYDIAS Chlamydia (arrows)

39. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Archaea Archaea share certain traits with bacteria and other traits with eukaryotes

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41. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Some archaea live in extreme environments Extreme thermophiles thrive in very hot environments Extreme halophiles live in high saline environments

42. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Methanogens live in swamps and marshes and produce methane as a waste product

43. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 27.4: Prokaryotes play crucial roles in the biosphere Prokaryotes are so important to the biosphere that if they were to disappear, the prospects for any other life surviving would be dim

44. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Chemical Recycling Prokaryotes play a major role in the continual recycling of chemical elements between the living and nonliving components of ecosystems Chemoheterotrophic prokaryotes function as decomposers, breaking down corpses, dead vegetation, and waste products Nitrogen-fixing prokaryotes add usable nitrogen to the environment

45. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Symbiotic Relationships Many prokaryotes live with other organisms in symbiotic relationships In mutualism, both symbiotic organisms benefit In commensalism, one organism benefits while neither harming nor helping the other in any significant way In parasitism, one organism, called a parasite, benefits at the expense of the host

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47. Concept 27.5: Prokaryotes have both harmful and beneficial impacts on humans Some prokaryotes are human pathogens, but others have positive interactions with humans

48. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Pathogenic Prokaryotes Prokaryotes cause about half of all human diseases Lyme disease is an example

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50. Pathogenic prokaryotes typically cause disease by releasing exotoxins or endotoxins Exotoxins cause disease even if the prokaryotes that produce them are not present Endotoxins are released only when bacteria die and their cell walls break down Many pathogenic bacteria are potential weapons of bioterrorism

51. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Prokaryotes in Research and Technology Experiments using prokaryotes have led to important advances in DNA technology Prokaryotes are the principal agents in bioremediation, the use of organisms to remove pollutants from the environment

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53. Some other uses of prokaryotes: – Recovery of metals from ores – Synthesis of vitamins – Production of antibiotics, hormones, and other products