1. Development of microsatellite markers for Puccinia psidii, a rust fungus threatening ohia (Metrosideros polymorpha) and other Myrtaceae in Hawaii
Shaobin Zhong and Baojun Yang
Department of Plant & Environmental Protection Sciences
University of Hawaii at Manoa
Honolulu, Hawaii

2. Life cycle and host range of Puccinia psidii
(Glen M et al Australasian Plant Pathology 36: 1–16)
Host range: 20 genera and 71 species from Myrtaceae: Eucalypteae, Leptospermeae, Melaleuceae, Metrosidereae, Myrteae, Syncarpieae and Syzygieae (Glen et al. 2007)

3. Origin and Distribution of Puccinia psidii
1977
2005
origin

4. Puccinia psidii in Hawaii
2005

5. Metrosidero polymorpha
Rhodomyrtus tormentosa
Eugenia koolauensis
Melaleuca quinquenervia

6. Ohia is often one of the first plants to colonize new lava flows and is the major component in Hawaiian forest
Photo by C. Elevitch

7. The native Hawaiian honeycreeper ‘apapane on an ‘Ohi‘a lehua

8. Questions to be answered:
How many races (or strains) have been introduced into Hawaii?
Where were these races from?

9. SSR-enriched DNA library
DNA sequencing
216 clones
Primer design
80 primer pairs
Evaluation of SSR markers
15 SSRs
Population genetics

10. HI Florida HI Brazil HI PsSSR12C
PsSSR12

11. HI Florida HI Brazil HI PsSSR195C
PsSSR195

12. HI Florida HI Brazil HI PsSSR208C
PsSSR208

13. Dendrogram based on 71 alleles from 15 SSR loci
100 85
100 93
100
Dendrogram based on 71 alleles from 15 SSR loci

14. Summary: Fifteen microsatellite markers were developed
Seventy one alleles were identified among 19 isolates analyzed
Three genotypes found in four isolates from Florida and two genotypes detected in four Brazil isolates
Only one genotype was found in 12 Hawaiian isolates

15. Acknowledgements:
Acelino Alfenas (Federal University of Viosa, Brazil)
Robert Hauff (Hawaii Department of Agriculture, Hawaii)
Eloise Killgore (Hawaii Department of Agriculture, Hawaii)
Min Rayachhetry (US Forest Service, Florida)
Les Szabo (CDL-USDA, Minnesota)
Janice Uchida (University of Hawaii, Hawaii)