1. Redefining DNA Microarrays Tiffany McAninch Advised by: Dr. Frederick Haselton, PhD Mark McQuain, 2BPhD

2. Purpose z If humanity was able to understand genetics more completely and able to determine what the causes were for many disorders, there would be a huge sweep for this information. DNA microarrays are the most recent tool to put this information to use in a large scale way. This project has been to design a system similar to microarrays, but one in which the information can be obtained faster, more accurately, and more easily.

3. Northern Blotting

4. Microarray Procedure zTarget genes prepared and hyb. onto slides zCulture cells zIsolate mRNA zPrepare labeled cDNA z2 cDNAs competitively hybridized zAnalyzed by fluorescent signature

5. Microarray Schematic

6. Streptavidin Beads

7. Flow Cytometer PMT Dichroic Filters Bandpass Filters Laser Flow Manifold SSC FSC Cells

8. Raman Spectroscopy

9. Comparison zMicrobeads yEasier to store yDo chemically yHold patent! yLess Reagents ySpeed yFlow Cytometer y2 million signatures z Microarrays yDon’t need Raman tag yNo flow cyt. for Raman y50,000 signatures

10. Conclusion z The design has been formulated, which was the purpose of this project. There has also been much research that will aid in future use of narrowing the design further. Having found the beads with suitable surface chemistries and measured the Raman of the tags, the project is ready to be further tested.

11. Thanks!! zProfessor Haselton zMark McQuain zProfessor Mahadevan-Jansen zChad Lieber zTodd Monroe