1. Effect of Germination Time on Rate of Respiration Mark Armanious, Sharon Liu, Simmy Panesar, Alex Rucker

2. Problem/Hypothesis How is the rate of respiration of peas affected by the amount of time they have had to germinate? Peas that are allowed to germinate longer will have a higher rate of respiration.

3. Background Cellular respiration o Consumes oxygen, glucose o Produces carbon dioxide Seeds o Contain stored energy source that is consumed when the seed germinates Germination o Plant begins to grow, using the stored energy o Hypothesis: As the plant grows, so will its energy requirements, its rate of cellular respiration, and therefore the consumption of oxygen

4. Procedure ●Test tube contains: ○ Absorbent cotton soaked with 15 drops KOH solution ○ Non-absorbent cotton as a buffer ○ Peas or glass beads ○ Stopper connected to pressure probe ●Trials were run for 20 minutes ○ 3 Trials per condition ●Variables ○ outside temperature and pressure kept constant ●Conditions: ○ Glass beads ○ 0-4 days germination overnight ■Glass beads were used to keep a constant volume

5. Raw Data Graph 1: Germination time of 0 and 4 Days Graph 3: Germination time of 3 Days Graph 2: Germination time of 1 Day Graph 4: Germination time of 2 Days

7. Discussion/Future Work ●Hypothesis was correct: ○ As germination period increased, the rate of respiration increased. ●Allows for characterization of different plants based on germination rate ●Does the rate of respiration level off after a certain time? ●Do different species display the same property?