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Genetic Engineering
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Electrophoresis & Cloning
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Miscellaneous
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Sex- Linked Traits
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Pedigrees
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Genetic Disorders
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DNA that is made up of pieces of DNA from different sources is called?
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Recombinant DNA
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The process where one organism (usually bacteria) is transformed by a gene(s) from another source ?
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Transformation
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An organism that has a functional piece of DNA is called?
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Transgenic Organism
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What type of proteins are used to cut DNA?
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Restriction Enzymes
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What are the 3 steps to manipulating DNA?
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1.Extracting DNA 2.Cutting DNA 3.Separating DNA
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Producing a genetically identical offspring from a single cell is called?
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Cloning
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Who was the first person to clone a mammal?
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Ian Wilmut
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How does Gel Electrophoresis separate DNA?
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By Size
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What charge is carried by DNA and how do you know?
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Negative. Because it moves toward the positive end.
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In the picture are the 3 samples in A identical, explain.
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No, because the banding patterns do not match or line up.
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The process where breeders choose the organisms with the most desired traits
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Selective Breeding
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What is mating between closely related individuals called?
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Inbreeding
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The offspring of parents that have different traits are called?
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Hybrids
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What is B a picture of?
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DNA
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What is going on in this picture?
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Restriction enzymes are cutting DNA into pieces.
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What are sex-linked traits (definition please no examples)?
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Genes/traits found on the sex chromosomes.
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How many sex chromosomes are there are what are they?
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2 X and Y
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In class we discussed 3 sex- linked traits name 2?
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Red-Green Colorblindness Hemophilia Muscular Dystrophy
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Why is it that the male is the one that usually gets the recessive trait?
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The male only has one X chromosome which is donated by the mom, there is nothing to mask the trait(s) on that X chromosome.
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If a normal male and a carrier female (red-green colorblindness) had a child what is the percent chance that the child will be red-green colorblind and what would the sex of the child be?
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25% chance of being a red- green colorblind male, 0% chance for a colorblind female XRXR Y XRXR X R X R Y XrXr X R X r X r Y
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A male is represented by what shape?
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Square
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A way of tracing a trait through a family history.
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Pedigree
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Individuals half shaded in are known as?
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Carriers
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The pedigree shows the inheritance of free earlobes and attached. Attached earlobes is caused by a recessive allele (f). How many generations are shown in this picture?
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5
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What are the genotypes of individuals 2, 8, 9, 5?
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Individuals 2,8,9 are Ff and 5 is FF
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Are most genetic disorders dominant or recessive?
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Recessive
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Which genetic disorder that we learned about was a co-dominant disorder? (hint: occurs mainly in African Americans)
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Sickle Cell Anemia
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This disorder causes the person to produce too much mucus or is unable to break it down.
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Cystic Fibrosis
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In this disorder the individual cannot break down the amino acid Phenylalanine or (Phe)
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Phenylketonuria or (PKU)
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Most sex chromosome disorders (ex. Jacob’s, Turner’s, Edward’s syndromes) are caused by what issue?
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Nondisjunction or the failure of the chromosomes to separate.
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