1. A. Malcolm Campbell Biology and GCAT Laurie J. Heyer Mathematics and GCAT AP Institute June 29, 2011 Introduction to Synthetic Biology Todd T. Eckdahl Biology and GCAT Jeff L. Poet Mathematics and GCAT

2. What is Synthetic Biology? Application of engineering principles and mathematical modeling to the design and construction of biological parts, devices, and systems with applications in energy, medicine, and technology. www.bio.davidson.edu/projects/gcat/Synthetic/What_Is_SynBio.html

3. Synthetic Biology: Win-Win Win #1: your design functions as expected.

4. Win #2: your design fails but you uncover basic biology Win #1: your design functions as expected. Synthetic Biology: Win-Win Research

5. Abstraction Modularity Standards Designing and modeling How is Synthetic Biology Different?

6. Abstraction

8. USB ports on computers Modularity

10. http://openwetware.org/images/b/bd/BBFRFC9.pdf Standardization On a Uniform System of Screw Thread William Sellers April 21, 1864 “In this country, no organized attempt has as of yet been made to establish any system, each manufacturer having adopted whatever his judgment may have dictated as best, or as most convenient for himself.”

11. On a Uniform System of Screw Thread Standardization

12. Modeling of Designs

13. Real World Applications of Synthetic Biology

14. Land Mine Detection

16. Synthetic Biology Land Mine Detection

17. Production of Medicines $1 per pill

18. Production of Medicines 10¢ per pill

19. Biofuels from Algae CO -neutral 2 1,000,000 gallons in 2008

20. Synthetic Biology Biochemistry Synthetic Biology Computer Science Engineering

21. Synthetic Biology

22. partsregistry.org Synthetic Biology

23. Anderson et al. Mol Sys Bio. 2007. Synthetic Biology

24. UC Berkeley iGEM, 2007 Synthetic Biology

25. BioBricks BioBrick Part BioBrick plasmid backbone BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS (http://partsregistry.org/Plasmids)

26. Eco RI BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS (http://partsregistry.org/Plasmids) Spe I Pst I Xba I BioBricks

27. Eco RI BioBrick plasmid backbone + 1 part BBa_B0015 origin antibiotic resistance X E PS (http://partsregistry.org/Plasmids) Spe I Pst I Xba I identical sticky ends BioBricks

28. PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S put B downstream of A BioBricks

29. PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S cut with Spe and Pst cut with Xba and Pst BioBricks

30. P S Part A originantibiotic resistance X E Part B X P S gel purify EtOH precipitate BioBricks

31. P S Part A originantibiotic resistance X E Part B X P S ligate BioBricks

32. S Part A originantibiotic resistance X E Part B X S P M mixed site = scar transform BioBricks

33. S Part A originantibiotic resistance X E Part B X S P M PS Part A originantibiotic resistance originantibiotic resistance E X E Part B X P S BioBricks

34. PS Part A originantibiotic resistance originantibiotic resistance E X E Assignment: Part B X P S put A upstream of B