1. H.Ghaderian1
1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , /155 , Esfahan , Iran

2. :Classification Family : Herpes viridea Sub family : herpes virinea
Genus : simplex
Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690
common name : herpes symplex virus

6. tegument

7. Alpha herpes viruses
latently infected

8. Herpes virus in the host cell

9. HSV of the newborn is acquired 3 periods: in utera Peripartium (85%) postpartum

10. Incidence of neonatal herpes in 3 form :
1) lesion of the skin , eyes , mouth

11. 2) Encephalitis with or without lesion

12. 3) Dissiminated desease in liver lunges gland adrenl gut

13. Neonatal herpes case of neonatal herpes HSV -1and HSV-2
Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause
Serologic investigations showed no IgM and IgG antibodies for HSV-1 and HSV-2 in new born

14. Immune ResponsesNeonatal
Infected neonates produce HSV-specific IgM antibodies
within 3 weeks of acquisition of the viral infection,
which increase rapidly during the first 2 to 3 months
and may remain for as long as 1 year after neonatal infection develops.

15. Lymphocytes from infected infants
alpha-interferon
Neonates INF –αcompare
with adults with primary HSV infection is decreased.

16. Study showed :
The first time infection of the pregnant women may lead to severe illness in pregnancy and may be virus transmission to newborn.

17. DIAGNISTIC
1)PCR
The high risk for death requires prompt diagnostic therfore suggestive that of HSV was identified by PCR
Viral DNA was extracted from all specimens
(tracheal aspirate, liver, lungs, and gut)
By using the QIAamp DNA Mini Kit

18. primers for the HSV-1: thymidinekinase gene
((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′
(Rev 5′-TTTTCTGCTCCAGGCGGACT-3′)
Primer for the HSV-2: DNA polymerase gene
((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′
(Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′)
The DNA was then used for HSV DNAPCR

19. Nucleotide sequence analysis
showed identical sequences in different specimens.
GenBank BLAST tool
highest similarity was
HSV-1 strain CL 101 and HSV-2 strain KN 53690

20. 2)Cell culture
• Multi nucleated epithelial cells

21. treatment All infants with diagnosed HSV infection
must be treated with an intravenous therapy with acyclovir
(60 mg/kg/day)
Antibody Therapy
Antibodies against the surface glycoproteins gB and gD prophylactic and therapeutic agents in HSV infection

22. Monophosphte acyclovoir
mechanism acyclovir
inactive acyclovir
Viral thimydin kinas
Monophosphte acyclovoir
cellular thimydin kinase
active )) acyclovir three phosphate
is analog to guanin blocked DNApolymerasefunction

23. PREVENTION
Cesarean Delivery cesarean delivery in a woman with active genital lesions can reduce risk of acquiring HSV to infant Antiviral Prophylaxis During Pregnancy the use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety

24. Hsv vaccine HSV-2 : glycoprotein D subunit
vaccine adjuvanted :monophosphorylipid A(MPL)
efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.

25. Thank you for your Attention