1. A convenient system for highly specific and sensitive detection of miRNA expression Xiangqi Li, Minjie Ni, Chaobao Zhang, Wubin Ma, Yonglian Zhang SUPPLEMENTAL DATA

2. A B C miR-138 miR-99b ＊ miR-125a-3p miR-291 miR-292-5pmiR-295 miR-326 miR-3085 NO miR-122Marker miR-138 miR-99b ＊ miR-125a-3p miR-291 miR-292-5pmiR-295 miR-326 miR-3085 NO miR-122Marker miR-138 miR-99b ＊ miR-125a-3p miR-291 miR-292-5pmiR-295 miR-326 miR-3085 NO miR-122Marker Fig. S1 Detection of miRNAs under different conditions by stem-loop PCR. 50bp 100bp 50bp 100bp 50bp 100bp

3. Fig. S2 The sensitivity of the detection of miR-7578 and distinguishing miR-7578 from its precursor by northern blotting. Rno-miR-7578 Pre-miR-7578 10 fmol 1 fmol1 amol1 zmol JEG-3 Cell MG-63 CellU-2 OS cell5637 Cell T24 Cell Rno-miR-29a by aLHCD Rno-miR-29a by northern blotting A B Fig. S3 Detection of miR-29a in different cell lines by different methods.

4. Fig. S4 Quantitative detection of miRNA levels using U6 as an internal control by LH-PCR.

5. Fig. S5 Evaluation of LH-PCR amplification efficiencies of miRNA and its precursor.

6. Fig. S6 Evaluation of LH-PCR amplification efficiencies of miRNA and its family members.

7. miR-29a miR-29b miR-29c Tissue RNA Fig. S7 Detection of miR-29 family members by conventional Northern blotting. Rno-miR-138 preHelaliverkideylungbrain Fig. S8 Detection of miR-138 and its precursor from tissue samples by stem loop-PCR.