1. THE ACTIVITY BALANCE OF PLATELET COLLECTION BY APHERESIS OVER A PERIOD OF 72 MONTHS (2006-2011) AT SINGLE CENTER IN ALGERIA R.Belhadj; R.Ahmed-nacer; M.Benakli; N.Rahmoune; F.Mehdid; A.Talbi M.Baazizi; D.Ait- Ouali; S.Akhrouf; F.Mehenou; RM.hamladji. Department of Hematology and BMT unit, Pierre and Marie Curie Center, Algiers, Algeria.

2. INTRODUCTION The platelet transfusion is a surrogate therapeutic way to offset a deficit induced by disease or cytotoxic therapy. Platelet transfusion can be a life-saving procedure in preventing or treating serious complications from bleeding and hemorrhage in patients who have disorders manifesting as thrombocytopenia or platelet dysfunction. This transfusion is a necessary and constant part of hematologic reanimation. We present the activity balance of platelet collection in our center.

3. Material and methods Period 72 months : (2006-2011): 5548 sampling platelet concentrate units PCU (924 / year). Origin of donations (PCU): family + + ++ volunteers

4. Material and methods Terms of sampling:  Personal qualified.  Questioning.  Physical examination.  Laboratory study: complete blood count: rate plat ≥ 150×10 9 /l Blood group. Viral serology: VIH, HbS, CVH, Syphilis (negative)  The platelet concentrate unit performed on a single donor thanks to a automated cell separator model TRIMA.

5. Material and methods TRIMA Accel : N= 3Agitator: N=2 Continuous flow separator. BV extracorporeal low. A Better hemodynamic tolerance continuous Horizontal agitation. Conservation 5-7 days. Temperature 22 0 C

6. Material and methods DISTRIBUTION BY GENDER: male predominance (93,2%) ONLY 379 (6.8%) are female P50 Amiri Nadia TUNISIA:(97,5% donors is the young males) 673 1452 1272 1232

7. Material and methods REPARTITION ACCORDING TO THE TYPE OF DONATIONS Donations Volunteers: 512 (9.2% )

8. RESULTS N = 5548 Before apheresisAfter apheresisLoss (%) Hematocrit (%) (average) 41,9 (30,1 – 57,6)40,5 (30 – 56 ) 3,34 rate plat (10 9 /l) (average) 262 ( 150 – 491)163 (79 – 335) 37 BLOOD DONORS RATES BEFORE AND AFTER APHERESIS

9. RESULTS N = 5548VALUES AFTER APHERESIS Volume deals average (ml) 3479 ( 890 – 5213) Volume of ACDA average (ml) 314,1 (91 – 505 ) Collection volume bag average (ml) 386 (50 -647) Plat. average yield / bag (10 11 ) 5,8 ( 1 – 8) Duration of procedure (min) 63,71 (19 – 120 ) VALUES OF PROCEEDINGS GIFT OF PLATELET CONCENTRATE UNITS

10. RESULTS Cancelled samples N = 76 (1.35%): poor donor tolerance /poor venous access

11. RESULTS UNITS USERS NUMBER % STERILE UNIT (BMT/ INDUCTION CT) 3495 63 GENERAL HEMATOLOGY CENTER 1110 20 HEMATOLOGY DAY HOSPITAL / CONSULTATION 888 16 EXTRA MURAL CENTER 63 1 TOTAL 5548 100 DISTRIBUTION OF USER UNITS

12. RESULTS Number of donation Donors volunteers AGAINST PART BTC/ T-O (2005- 2009) 47.77414 %86 % OUR CENTER ( 2006-2011) 55489,2 %90,8 % L.Ainas Revue Algérienne d’hématologie N° 5 septembre 2011: prévalence des infections VIH; VHC et Syphilis chez les donneurs de sang au CTS de (2005-2009 ) COMPARISON RATES OF VOLUNTEERS DONORS IN BLOOD COMPONENT : PCU : our center / Red Blood Unit : Blood Center tranfusion (BTC) Tizi-ouzou (TO)

13. DISTRIBUTION OF PUC / YEAR IN ALGERIA : Multicentric study of 1 year (2009) BTC PLATELETS SAMPLS % NUMBER OF MACHINS FOR CYTAPHERESIS OUR CENTER 1452 543 UHC Oran 504 18,741 UHC SBA 340 12,642 UHC Batna 125 4,641 UHE Oran 119 4,421 Beni Messous 94 3,491 Tizi-Ouzou 45 1,71 BTC Blida 10 0,371 TOTAL 2689 10011 S. Nekkal, M.Belhani. Revue Algérienne d’hématologie N° 5 septembre 2011 : la transfusion de plaquettes en hématologie: le point en 2009 Etude multicentrique

14. Conclusion The platelet transfusion is very important in hematologic reanimation. Despite the large number of donations (PCU) per year; it remains insufficient because of the lack of voluntary donors especially in summer and the month of Ramadan.

15. THANK YOU