1. Photosynthesis - Chapter 8

2. Spinach Chromatography As the alcohol travels up the filter paper it carries leaf pigments. The small pigments travel farthest and fastest. The largest pigments travel slow and stay close to the start. Chlorophyll a  Chlorophyll b  Xanthophyll  Carotene 

3. 8-1 Energy and Life Energy is the ability to do work Living things depend on energy to maintain homeostasis Without the ability to obtain and use energy, life would cease to exist Where does this energy come from?

4. Chemical Energy and ATP Energy comes in many forms Exs. – light, heat and electricity ATP and ADP –Cell activities are powered by chemical fuels –One of the principal chemical compounds that living things use to store and release energy is ATP (adenosine triphosphate)

5. An ATP molecule consists of a nitrogen-containing compound called adenine, a 5-carbon sugar called ribose, and three phosphate groups pg. 202 Fig. 8-2 ATP is used by all types of cells as their basic energy source. For example, the energy needed by the cells of a soccer player comes from ATP.

6. ADP (adenosine diphosphate) has a structure that is similar to ATP There is one important difference: ADP has two phosphate groups instead of three This is the key to the way in which cells store energy

7. A cell can store small amounts of energy by adding a phosphate group to ADP molecules, producing ATP molecules ATP is like a fully charged battery, ready to power the machinery of the cell.

8. pg. 203

9. Releasing Energy From ATP Energy stored in ATP is released when ATP is converted into ADP and a phosphate group Cells can add and subtract a third phosphate group giving it a way of storing and releasing energy as needed.

10. Most cells have only a small amount of ATP, enough to last for only a few seconds of activity ATP is a great molecule for transferring energy. It is not good for long term energy storage

11. The ATP Cycle

12. ATP carries just enough energy to power a variety of cellular activities –Exs.: Active Transport (Protein Pumps) Movement (cilia, flagella, muscles) Light (Fireflies) Using Biochemical Energy

13. The characteristics of ATP make it an exceptionally useful molecule that is used by all types of cells as their basic energy source

14. Autotrophs and Heterotrophs Originally, nearly all energy in food comes from the Sun. The energy that living things need comes from food

15. Autotrophs are organisms such as plants, which make their own food Heterotrophs obtain energy from the foods they consume –Ex.: Impalas, leopards, & mushrooms

16. Fig. 8-1 Autotrophs vs. Heterotrophs Autotrophs use light energy from the sun to produce food. These impalas get their energy by eating grass. A leopard, in contrast, gets its energy by eating impalas and other animals. Impalas and leopards are both heterotrophs. pg. 201

17. Photosynthesis is a process in which plants use the energy of sunlight to convert water and carbon dioxide into oxygen and high-energy carbohydrates (sugars and starches) that can be used as food. 8–2 Photosynthesis: An Overview

18. An Overview of Photosynthesis

19. The overall equation for photosynthesis can be shown as follows: 6CO 2 + 6H 2 O sunlight C 6 H 12 O 6 + 6O 2 chlorophyll carbon dioxide + water sugar + oxygen

20. Chlorophyll and Chloroplasts Energy from the Sun travels to Earth as light. Our eyes perceive this as “white light” which is actually mixture of different wavelengths. We see these wavelengths as: Red, Orange, Yellow, Green, Blue, Indigo and Violet.

21. In addition to water and carbon dioxide, photosynthesis requires light and chlorophyll, a molecule in chloroplasts Plants gather the sun’s energy with light- absorbing molecules called pigments The plants’ principal pigment is chlorophyll There are two main types of chlorophyll: –chlorophyll a –chlorophyll b

22. Chlorophyll absorbs the blue and red regions of the visible spectrum Chlorophyll does not absorb light well in the green region of the spectrum This is why plants are green Plants also contain red and orange pigments that absorb light in other regions of the spectrum –Ex.: carotene

23. Fig. 8-5 Photosynthesis requires light and chlorophyll, which absorbs light energy. In the graph, notice how chlorophyll a absorbs light in the violet and red regions of the visible spectrum, while chlorophyll b absorbs light in the blue and red regions of the visible spectrum. pg. 207

24. Inside a Chloroplast –Photosynthesis takes place inside chloroplasts –Chloroplasts contain saclike photosynthetic membranes called thylakoids Chloroplasts

25. Thylakoids: – Are arranged in stacks known as grana (singular: granum) –The fluid portion of the chloroplast outside the thylakoids is known as the stroma. –Contain clusters of chlorophyll and other pigments and photosystems (proteins that are able to capture the energy of sunlight.

26. High-Energy Electrons Sunlight excites electrons in chlorophyll and the electrons gain a great deal of energy These high-energy electrons require a special carrier

27. One of these carrier molecules is a compound known as NADP+ The conversion of NADP+ into NADPH is one way in which some of the energy of sunlight can be trapped in chemical form.

28. Fig. 8-4 Photosynthesis is a series of reactions that uses energy from the sun to convert water and carbon dioxide into sugars and oxygen. Photosynthesis takes place in a plant organelle called the chloroplast. pg. 206

29. The photosynthesis reaction has two stages: –The Light-Dependent reactions take place within the thylakoid membranes –The Light-Independent reactions (Calvin Cycle) The Calvin Cycle takes place in the stroma, the region outside the thylakoid membranes.

30. The Process of Photosynthesis Light-Dependent Reactions Light-Independent Reactions Light & H 2 O CO 2 ATPNADPH O2O2 C 6 H 12 O 6

31. pg. 209

32. Light-Dependent Reactions (ATP and NADPH) Require light Why plants need light to grow Use energy from light to produce oxygen gas, ATP and NADPH 8–3 The Process of Photosynthesis

33. Light-Independent Reactions (Sugars) The light-independent (dark) reactions use ATP and NADPH from the light-dependent reactions to produce high-energy sugars These reactions are also called the Calvin Cycle The Calvin Cycle does not require light

34. pg. 212

35. Factors Affecting Photosynthesis Many factors affect the rate at which photosynthesis occurs

36. Temperature: –Temperatures above or below 0°C and 35°C may slow down the rate of photosynthesis –At very low temperatures, photosynthesis may stop entirely –Plants at these temperatures can carry out photosynthesis only on sunny days

37. Intensity of Light – Affects the rate at which photosynthesis occurs. –Increasing light intensity increases the rate of photosynthesis –At a certain level, the plant reaches its maximum rate of photosynthesis –This level varies from plant to plant.

38. Water: –A shortage of water can slow or even stop photosynthesis –Plants that live in dry conditions (desert plants and conifers) have a waxy coating on their leaves that reduces water loss

39. Photosynthesis Under Extreme Conditions Plants have small openings (stomata) in their leaves that admit CO 2 for photosynthesis To prevent the plant from drying out, these openings must close to conserve water. This may slow down or stop the process of photosynthesis

40. C4 and CAM Photosynthesis Some plants have adapted to extremely bright, hot conditions There are two major groups of these specialized plants: –C4 plants –CAM plants These processes minimize water loss while still allowing photosynthesis in intense sunlight

41. C4 plants have an added step during the carbon-fixing stage to preserve moisture in hotter climates. C4 plants capture CO 2 so that plants can keep working under intense light and high temperatures. C4 Plants: –Corn –Soy Beans –Sugar Cane –Crabgrass

43. CAM plants take in CO 2 only at night, trapping carbon in the leaves. During the day, when the openings in the leaves are tightly closed, the CO 2 is released allowing photosynthesis to take place. CAM Plants: –Pineapple –Cactus