1. Who's
Hyped?

2. Purpose
I would like to test the effects of energy drinks on the heart rate of Daphnia Magna.

3. Real Life Connection
Teens are drinking a lot of energy drinks these days. Some people have died from them. I am trying to find the dangers of energy drinks in my experiment.

4. Research
There were studies at the University of Miami Concerning the use of energy drinks. The lead pediatrician was Dr. Steven E. Lipshultz. According to studies 30-50% of adolescents are drinking energy drinks regularly. The energy drink market was supposed to have reached over 9 billion dollars last year.

5. Research (continued)
In many news reports it has cautioned parents against energy drinks due to the amount of caffeine in these drinks. The healthy daily limit for a twelve year old is about 100mg every day. Most energy drink companies claim that they are dietary supplements and, therefore, are not monitored by the Food and Drug Association (FDA) like soft drinks.

6. Mg of Caffeine per fl. Ounce
Research (continued)
Drink
Mg of Caffeine per fl. Ounce
Monster
10.0mg
Amp
8.875mg
Red Bull
9.524mg
Nos
16.25mg
5-Hour
71.502

7. Research (continued) Daphnia Magna Diagram

8. Bibliography
Lipshulz, Dr. Steven E. "New Report Shows Energy Drinks May Harm Children - ABC News." ABCNews.com - Breaking News, Latest News & Top Video News - ABC News. American Broadcasting Company, Web. 27 Dec <
N.A. "Caffeine Content of Drinks." Energy Fiend - The Caffeine Fix. Energy Freind, Web. 28 Dec <
N.A. "Working with Daphnia » Ward's Science Teacher ResourcesWard's Science Teacher Resources." Home - Ward's Science Teacher ResourcesWard's Science Teacher Resources. Ward's Natural Science. Web. 1 Jan <

9. Hypothesis
I think the 5 Hour energy drink will have the most effect on the Daphnia Magna in quantity and singularly because of the amount of mg of Caffeine per fl. oz.

10. Materials 6 pipettes A sharpie A microscope 15 microscope slides
One can of Amp
One can of Monster
One can of Nos
One can of Red Bull
One bottle of 5-hour
Microscope Camera
Computer
15 Daphnia Magna

11. Procedure Hook up camera and microscope to computer
Label 5 of the pipettes with the names of the energy drinks
Label the last pipette Daphnia
Use the daphnia pipette to put a daphnia on the slide
Record the daphnia using the camera for one minute making sure to be able to see the heart the whole time
Add one drop of monster to the slide using the monster pipette
Record the daphnia using the camera for one minute making sure to be able to see the heart beat the whole time

12. Procedure (Continued)
8. Add another drop of monster to the slide using the monster pipette
9. Record the daphnia using the camera for one minute making sure to be able to see the heart beat the whole time
10. Continue this for 5 drops then repeat for three trials
11. Repeat steps 4-9 for each energy drink being sure to use different slides for each daphnia and different pipettes for each drink

13. Monster
Heart Beats per Minute
Drops of Energy Drink

14. Amp
Heart Beats per Minute
Drops of Energy Drink

15. Nos
Heart Beats per Minute
Drops of Energy Drink

16. Red Bull
Heart Beats per Minute
Drops of Energy Drink

17. 5 Hour
Heart Beats per Minute
Drops of Energy Drink

18. Data Chart Beats Per Minute
Drink Trial Base 1drop 2 drops 3 drops 4 drops 5drops
Monster
Monster
Monster
Amp
Amp
Amp
Nos
Nos
Nos
Red Bull
Red Bull
Red Bull
5 Hour
5 Hour
5 Hour

19. Results
I saw that the Red Bull had very little affect on the Crustaceans. I also saw that with most of the trials the heart rate went up but by the 3rd drop it started to slow down a lot and stay there. The Monster and Amp slowed it down but it wasn’t at a dangerous level. The 5 Hour on the other hand slowed the hearts down and stopped 2 out of the 3. When they got the Nos they went up and stayed up through out the trials

20. Conclusion
In conclusion it was a tie for the most affect between 5 hour and Nos. The 5 Hour slowed their hearts down a whole lot and the Nos sped them up.
If I could do my project again I think I might observe the rates long term to see if the energy drinks just went through the system or stayed there. In the future I will separate the ingredients and see how the different ingredients affect them.

21. Summary
In my experiment I wanted to see the affects of the energy drinks on Daphnia Magna. I thought it would speed up the heart rates until I stopped but I thought wrong the heart rates sped up but then slowed down. This is why these drinks are dangerous. The drink might boost your energy but, in turn it might slow your heart down until it stops.

23. Pictures
Heart

24. More Pictures
Heart

25. Another Picture