2. Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction Enzymes Gel Electrophoresis Confidentiality

3. Question Answer Careers-100 ANSWER: The individual performs DNA profiling. QUESTION: Who is a DNA analyst?

4. Question Answer Careers-200 ANSWER: A physician who performs an autopsy when death may be accidental or violent. QUESTION: Who is a medical examiner?

5. Question Answer Careers-300 ANSWER: This individual performs scientific tests on bodily fluids and tissue samples to identify any drugs or chemicals present in the body. QUESTION: Who is a toxicologist?

6. Question Answer Careers-400 ANSWER: This individual provides medical treatment at the scene of a crime or on the way to a hospital. QUESTION: Who is an emergency medical technician (EMT)?

7. Question Answer Careers-500 ANSWER: This individual pronounces death and signs the death certificate. QUESTION: Who is a coroner?

8. Question Answer ANSWER: This person is responsible for notifying the appropriate responders. QUESTION: What is a 911 operator? Grab Bag-100

9. Question Answer Grab Bag-200 ANSWER: Demonstrating reliability, give credit to the author, as a source for additional information. QUESTION: What are reasons to document (cite) your sources?

10. Question Answer Grab Bag-300 ANSWER: Larger pieces of DNA will travel _____________ than small ones in a gel. QUESTION: What is slower?

11. Question Answer Grab Bag-400 ANSWER: To help determine sizes of unknown DNA fragments. QUESTION: Why do scientists load DNA of known sizes into the agarose gel?

12. Question Answer Grab Bag-500 ANSWER: This is the name given to the morgue assistant that helps prep the body for autopsy. QUESTION: What is the diener?

13. Question Answer Restriction Enzymes-100 ANSWER: A degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA. QUESTION: What is a restriction enzyme?

14. Question Answer Restriction Enzymes-200 ANSWER: Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes). QUESTION: What are Restriction Fragment Length Polymorphisms (RFLPs)?

15. Question Answer Restriction Enzymes-300 ANSWER: Segments of DNA cuts with restriction enzymes are measured in number of this. QUESTION: What is number of base pairs?

16. Question Answer Restriction Enzymes-400 ANSWER: Where restriction enzymes are found. QUESTION: What is bacteria?

17. Question Answer Restriction Enzymes-500 ANSWER: The restriction enzyme EcoR1 cuts a strand of DNA at four restriction sites resulting in this many RFLPs. QUESTION: What are five?

18. Question Answer Gel Electrophoresis-100 ANSWER: In gel electrophoresis, DNA shows up as these on the gel. QUESTION: What are bands?

19. Question Answer Gel Electrophoresis-200 ANSWER: In gel electrophoresis, DNA is fragments are separated based on this. QUESTION: What is number of base pairs?

20. Question Answer Gel Electrophoresis-300 ANSWER: During gel electrophoresis, this causes DNA to move out of the wells and through the gel. QUESTION: What is the attraction of the negative DNA, from the phosphate groups, to the positive electrode?

21. Question Answer Gel Electrophoresis-400 ANSWER: During gel electrophoresis, this size fragments migrate further in the gel due to less friction with the agarose. QUESTION: What are smaller fragments?

22. Question Answer Gel Electrophoresis-500 ANSWER: This is added to a gel to separate different size fragments on DNA. QUESTION: What is an electric current?

23. Question Answer Confidentiality-100 ANSWER: A comprehensive set of standards and practices designed to give patients specific rights regarding their personal health information. QUESTION: What is the Health Insurance Portability and Accountability Act (HIPAA)?

24. Question Answer Confidentiality-200 ANSWER: Individually identifiable health information such as demographic data, mental/health condition, and payment information is considered this. QUESTION: What is protected health information (PHI)?

25. Question Answer Confidentiality-300 ANSWER: It is ok to do this when trying to prevent child abuse, domestic violence, or the outbreak of a disease. QUESTION: What is break confidentiality?

26. Question Answer Confidentiality-400 ANSWER: These individuals should keep patient information private. QUESTION: Who is any medical personnel and those who have access to medical records?

27. Question Answer Confidentiality-500 ANSWER: Violating patient confidentiality can possibly result in serious fines, criminal prosecution, and this. QUESTION: What is loss of employment?