1. Copyright 2008 © Silliker, All Rights Reserved Collection of Samples for Chemical Analysis

2. Copyright 2008 © Silliker, All Rights Reserved Outline Reasons for Sampling Sampling Protocols Controlling Variability Quantifying Variability Discussion

3. Copyright 2008 © Silliker, All Rights Reserved Reasons for Sampling Regulatory Compliance HACCP requirement Risk Assessment Product Quality Monitoring Response to Complaint

4. Copyright 2008 © Silliker, All Rights Reserved Sampling Protocols Will vary based on the reasons for sampling Consult with your analytical lab BEFORE sampling Follow regulatory requirements to a tee

5. Copyright 2008 © Silliker, All Rights Reserved Types of Samples Grab Samples Composite Samples Random Samples Targeted Samples Market Basket Samples Partial vs. Whole sample

6. Copyright 2008 © Silliker, All Rights Reserved Sampling Considerations Containers Sample sensitivity to ambient conditions (light, heat, air) Preservatives Holding Times

7. Copyright 2008 © Silliker, All Rights Reserved Water Sample Containers

8. Copyright 2008 © Silliker, All Rights Reserved Containers Flexible Rigid Plastic Glass Native Packaging (unopened if possible)

9. Copyright 2008 © Silliker, All Rights Reserved Preservatives Chemical Preservatives used primarily with water sampling Stabilize the target analyte by decreasing chemical or biological degradation May render sample unsuitable for other analytes Refrigeration vs Freezing Cooling vs. keeping cool

10. Copyright 2008 © Silliker, All Rights Reserved Example preservatives Sodium thiosulphate  Dechlorinator Nitric acid  Stabilizer for metals in water Sulphuric Acid  Stabilizer for ammonia, oil and grease

11. Copyright 2008 © Silliker, All Rights Reserved Example Preservatives

12. Copyright 2008 © Silliker, All Rights Reserved Holding Time Clock starts at sampling Holding time is under specified conditions Usually taken to be the time when the variability due to degradation becomes significant compared to the expected variability of analysis

13. Copyright 2008 © Silliker, All Rights Reserved Example Preservatives and HOLD Times Volatiles in water  3 X 40 ml septum vial (no headspace)  Sodium bisulphate  14 days BOD in wastewater  1 litre  48 hours  Keep at 4 deg C.

14. Copyright 2008 © Silliker, All Rights Reserved Controlling Variability Sampling Methodology Preferred Lab ? Others factors

15. Copyright 2008 © Silliker, All Rights Reserved Sampling Data to be Recorded Who, When and Where  Record on CoC or traceable notes Environmental Conditions during sampling Any information that may influence results or interpretation of results

16. Copyright 2008 © Silliker, All Rights Reserved Quantifying Variability If we can’t control it, we must try to measure it Often as simple as noting changes in case they impact data Field duplicates Understanding “Measurement Uncertainty” in assessing your data

17. Copyright 2008 © Silliker, All Rights Reserved Questions? Contact Information  Walter Brandl  Silliker JR Labs  walter.brandl@silliker.com walter.brandl@silliker.com  604-432-9311