3. Cultivation To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.

4. Cultivation Bacteria Plant Animal Insect

5. Methods of animal virus cultivation 1.Organized tissue. 2.Cell culture. 3.Laboratory animals.

6. Chick embryo technique

8. Methods of animal virus cultivation 1.Organized tissue. 2.Cell culture. 3.Laboratory animals.

9. Cell culture 1.Removal. 2.Homogenization. 3.Washing. 4.Trypsinization. 5.Washing. 6.Growing. 7.Inoculation. 8.Incubation.

11. Methods of animal virus cultivation 1.Organized tissue. 2.Cell culture. 3.Laboratory animals.

12. IntracerebrallyIntraperitoneallyIntramuscularlyIntravenouslyIntranasally

13. Types of cell culture Primary Diploid Suspension Permanent

14. Primary cell culture A culture derived directly from a tissue

15. Diploid cell culture Subsequent cultures derived from primary cell culture.

16. Suspension cell culture A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.

17. Permanent cell line A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely.

18. Cultivation of plant viruses Protoplast and callus method. Mechanical method. Biological method.

19. Cultivation of plant viruses Protoplast and callus method. Mechanical method. Biological method.

20. InoculationIncubation InoculationIncubation

21. Cultivation of plant viruses Protoplast and callus method. Mechanical method. Biological method.

23. Cultivation of plant viruses Protoplast and callus method. Mechanical method. Biological method.

24. Incubation

25. Cultivation of bacterial viruses 1. On broth media 2. On solid medium

26. Bacteria + solid agar Virus + semisolid agar

27. Cultivation of Insect viruses

29. Criteria of purity Chemical purity Physical purity Biological purity