1. What do the FSU Seminoles call the elderly? Coach

2. UTexas’s Presidents Mansion

3. Research at UTexas

4. UTexas Dogs

5. UTexas Graduate

6. UTexas Driver

8. Kids of UTexas Grads

9. UTexas Repairmen

10. Sign at UTexas

11. UTexas Tailgating

13. How to get tickets for a Gator game

14. Are cats too tough for bedbugs?

15. Are Bed bugs too tough for Cats? Cat after bedbug attack!

16. CNN Bed Bug Video

17. Species of Bugs Cimex hemipterus, Tropical bed bug Moderately excavated Cimex lectularius, Bed bug Deeply excavated

18. Bed Bug vs Bat Bug Bat Bug, Cimex pipistrellaBed bug, Cimex lectularius

19. Bed Bug Development Gradual Metamorphosis Eggs –~1 mm long, whitish –Sticky, attached to surfaces –Laid in loose groups, ~200 per female Nymphs –5 instars –As small as 1 mm long –Tan almost transparent in color –At least 1 blood meal per instar

20. Adults Temperature affects life-span –At 37° C, females live an average of 32 days while males live an average of 29 days –At 10° C, females live an average of 425 days while males live an average of 401 days Females can lay between 200 and 500 eggs in their lifetime (Johnson 1942, Usinger 1966)

21. Bed Bug Life Cycle? Total time = 32-48 days 1 st Instar 0.34 mg (0.36 ul) (3-4 x body wt) 2 nd Instar 0.96 mg (1.01 ul) (4-5 x body wt) 3 rd Instar 2.08 mg (2.18 ul) (4-5 x body wt) 4th Instar 4.11 mg (4.32 ul) (4-5 x body wt) 5 th Instar 7.09 mg (7.44 ul) (2-6 x body wt) Adult Female 7.81 mg (8.20 ul) (2 x body wt) Adult Male 2.37 mg (2.49 ul) (1.5 x body wt) Eggs 1 mm long Adult longevity = ~1 month 550 days without a blood meal Laid in batches of 10-50 eggs 200-500 eggs total Hatch in 7-12 days 3-12 min feeding

22. Bed bug nymph feeding!!

23. Engorged!

24. With this Info… Approximately 750,000 bed bugs, feeding simultaneously will drain an average adult.

25. And we can further infer… Roughly 375K bed bugs can change a person from this… To this…

26. Bed Bug Gut Expandable Midgut Crop Malpighian tubules Proventriculus

27. Bites Bed bugs prefer to bite by not crawling onto host Painless, red papules, often in rows 1 st bite requires 10-14 days for immune reaction, later bites react faster Same Pattern Rows of bites

28. Medical Importance of Bed Bug Bites Sometimes: no reaction Most commonly: small inflamed pruritic bumps that resolve in 1-2 wk Allergic reaction: skin reaction resulting in urticaria (hives) evolving into fluid-filled rashes, Complicated by secondary bacterial infections. Rarely, asthma occurs and may be accompanied by more extensive urticaria and anaphylaxis No documentation of disease transmission –>20 pathogens have been found in bed bug guts –No evidence of bed bugs spreading HIV or any other disease

29. Other Potential Hosts Chickens Pigeons Cats Dogs Rodents

30. How do we feed them in the lab?

31. ½ of the blood ingested is lost by defecation within 5 hours.

32. Movement Quick movers –Adults can crawl about 4 ft/minute –Nymphs can crawl about 1 ft/minute 2½ minutes for an adult to make it from a TV stand to the pillow 10 minutes for a nymph

33. Close up of Bed Bug Leg Adapted for crawling. Claw used for gripping rough surfaces or for gripping host to insert mouthparts. Claw Tarsi

34. Leg of Cockroach Adapted for running and climbing. Claws for traction. Pads for climbing smooth surfaces. Claws Tarsi Pads

35. Cockroach Easily climbs smooth surfaces. Can’t climb smooth surfaces. Pads vs. No Pads Bed bug Site for pesticide absorption None here

36. Activity of the bed bug

37. How does this insect behavior make Gator fans more susceptible to bed bug bites? January 8 th, 2007 Glendale, Arizona VS April 3 rd, 2006 Indianapolis, IN

38. Primarily Nocturnal Activity Start activity around 7:30 pm

39. Primarily Nocturnal Activity Sharp rise in activity from 8:00 pm to midnight and later.

40. Primarily Nocturnal Activity Peak activity at 4:30 am

41. Primarily Nocturnal Activity Sharp decline in activity after 7:00 am

42. What makes them active? Hunger –search for host until one is found –Most likely follow CO 2 or heat Cannot detect a host beyond 5 feet. Attractants are being developed

43. Bed Bug Aggregations Pictures courtesy of Rick Cooper, Cooper Pest Solutions Aggregate (Usinger 1966) Cracks and crevices (Usinger 1966, Pinto 2007) Bed bug aggregations are chemically mediated by pheromones (Usinger 1966, Siljander et al. 2007)

44. Drywall Screw & Bed Bug Aggregation 15 bed bug nymphs in slots of drywall screw

45. Aggregations Possible Advantages –Safe harborages (Lorenzo and Lazzari 1996) –Mate-finding (Pinto et al. 2007) –Resist desiccation (Benoit et al. 2007) Possible Disadvantages (Wertheim et al. 2005) –Competition –Natural predators –Overuse of resources –Inbreeding Specific life stage that disperses  Specific life stage that disperses

46. Bed Bug Dispersal Disperse on belongings (Usinger 1966, Pinto et al. 2007) If they are dispersing, they are alone, not aggregated Why do they disperse from aggregations? Picture courtesy Rick Cooper, Cooper Pest Solutions Luggage with bed bug eggs

47. Mating Behavior Traumatic insemination- Male’s reproductive organ actually pierces the cuticle of the female’s abdomen and injects sperm through the wound, outside of the reproductive tract (Usinger 1966) Picture from Stutt and Siva-Jothy 2001

48. Problems caused by Bedbugs Nuisance Sleepless nights Inflamed skin lesions Stained sheets and mattresses Psychological stress from infested environment

49. Bed Bug Places Apartments, condos, houses Hotels, motels, restaurants Schools, universities, colleges Halfway houses Homeless shelters Correctional facilities Hospitals Nursing homes Subways, busses, taxis Discarded mattresses & furniture Movie theaters Homeless shelters Hospitals Busses Discarded mattresses Movie theaters Nursing homes

50. Bed Bug Places

51. Canine Bed Bug Detection

52. Can cats be huge pests?

53. Punk Cats

54. Is this the best method of control?