1. Commercial Assays and Detection of Parvovirus B19 genotypes Dr Sally A. Baylis, Division of Virology NIBSC

2. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

3. Fluorescence (F1/F2) Cycle Number Fluorescence (F2/Back - F1) M 1 1 3 3 2 2 NTC M Genotype 3 Genotype 1 Genotype 2NTC Cycle Number Region amplified: NS1 Roche Parvovirus B19 Quantification Kit

4. Cycle Number Fluorescence (F2/Back - F1) M 1 1 3 3 2 2 NTC M Genotype 3 Genotype 1 Genotype 2 NTC Region amplified: VP1 Artus RealArt TM Parvo B19 LC kit

5. Sensitivity of Detection of Different Genotypes of Parvovirus B19

6. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

7. Summary Choice of assay will affect outcome in terms of detection/quantification of different genotypes of parvovirus B19 Choice of assay will affect outcome in terms of detection/quantification of different genotypes of parvovirus B19 Choice of primers (and probe) effects ability to detect & quantify variant viruses Choice of primers (and probe) effects ability to detect & quantify variant viruses Maintain watching brief of databases to ensure adequate detection – science moves on Maintain watching brief of databases to ensure adequate detection – science moves on K. Hokynar et al. 2004 J. Clin. Microbiol. S. Baylis et al. 2004 J. Virol. Methods S. Braham et al. 2004 J. Clin. Virol. B. Cohen et al. 2006 J. Clin. Virol.