1. Agilent MassHunter Quantitation Analysis Software
Quant Method Assistant is a standalone Excel workbook that can dynamically generate a valid Quant method from a list of {CompoundName, MZ, RT,…}
Please refer to the video : Method Editor - Quant Method Assistant - Advanced.wrf
Agilent Technologies MassHunter
Quantitative Analysis Software.
All the performance, All the time

2. Text file Excel VBA macro Excel spreadsheet Mass Hunter Quant
Agilent MassHunter Quantitative Analysis Software Special Dynamically create Quant method using Excel VBA macro
Inputs {compound, MZ}
Text file
Load New Method from existing file and validate in Quant Method Editor
Excel spreadsheet
Excel VBA macro
Mass Hunter Quant
Quant Method
Quant Method Assistant is a standalone Excel workbook that can dynamically generate a valid Quant method from a list of {CompoundName, MZ, RT,…}
Steps shown…
Msxml library
Read {compound, MZ} and creates XML document
Write out deliverable: Quant method as XML document
XML doc
All the performance, All the Time

3. Dynamic Quant Method Creation
Target Compound information
Excel
Quant Method
' Purpose: Create a Quantitation Method '
' Assumptions:
' Column name match the TargetCompound schema column name
' Column values match the definitions in the QuantitationDataSet
' Steps:
' Create the XML document and protocol
' Read the columns/rows and write out the corresponding XML tagged value
' Each row represents one target compound in the quant method
' Each column represents the setting for that target compound
' Create a simple calibration table necessary for standard quantitation
' Save the document with quantmethod.xml as suffix
' Load this quant method manually into the Quant application
' Extensibility:
' The macro approach was designed for advanced users to extend the capabilities.
' For example:
' Add in additional TargetCompound columns such as SelectedMZ for MRM transitions
' Add in TargetQualifier ions transitions which requires the TargetQualifier table
' Add in ISTD relationships which requires Excel formula logic
' Add in globals such as RelativeISTD analysis
' Error Checking:
' The XML document is written as a set of tagged strings without validation
' Errors in the XML document are discovered when the quant method is loaded using
' Open Method from Existing File...
VBA macro writes Quant method (XML document) customizable
Excel reformats information from external system into loadable quant method.
Agilent Technologies MassHunter
Quantitative Analysis Software.
All the performance, All the time

4. Refer to video talking thru steps
' Purpose: Create a Quantitation Method '
' Assumptions:
' Column name match the TargetCompound schema column name
' Column values match the definitions in the QuantitationDataSet
' Steps:
' Create the XML document and protocol
' Read the columns/rows and write out the corresponding XML tagged value
' Each row represents one target compound in the quant method
' Each column represents the setting for that target compound
' Create a simple calibration table necessary for standard quantitation
' Save the document with quantmethod.xml as suffix
' Load this quant method manually into the Quant application
' Extensibility:
' The macro approach was designed for advanced users to extend the capabilities.
' For example:
' Add in additional TargetCompound columns such as SelectedMZ for MRM transitions
' Add in TargetQualifier ions transitions which requires the TargetQualifier table
' Add in ISTD relationships which requires Excel formula logic
' Add in globals such as RelativeISTD analysis
' Error Checking:
' The XML document is written as a set of tagged strings without validation
' Errors in the XML document are discovered when the quant method is loaded using
' Open Method from Existing File...
Agilent Technologies MassHunter
Quantitative Analysis Software.
All the performance, All the time