1. Day DateReadingTopic Th11/28no class – Thanksgiving Break T12/3Ch. 12more on Neurogenesis Th12/5Ch. 9 The Saga of the Germ Line T12/10more on Reproduction quiz on Tues: Draw and Describe pathfinding in any animal CNS Th12/12 don’t need to read Ch. 13Growth in class assignment on Thurs: build an Edible Urbilaterian Ancestor you can bring extra ingredients The Final Exam will be in our regular classroom on Friday, Dec. 20 th, 8:00 – 10:00 AM. The exam will cover all the material in the course. Also remember there are two, short writing assignments, each worth 20 points. Pick any topic in developmental biology (except your term paper topic) and learn more about it. Then write a two page paper and include all the sources you used (those can include any sources including web sites, newspapers, TV, etc). These are due anytime.

2. Final Exam covers the whole course

3. Made from Fig. 12.25

4. 12.18 growth cone guidance

5. Fruit fly embryonic CNS wild-type robo mutant comm mutant slit mutant (roundabout) (commissureless) (I faked this pic)

7. http://en.wikipedia.org/wiki/Urbilaterian http://palaeos.com/metazoa/bilateria.htm

9. Chapter 9: Reproduction The Saga of the Germ line cells Fertilization Sex determination Dosage compensation

10. 9.6 embryonic migration of germ line cells in mammals

11. migrating mouse germ line cells tagged with GFP

12. 1)We learned we can make a reporter gene construct by fusing any gene promoter of interest to the GFP transcription unit. When fused to the insulin promoter, for example, the hybrid gene would be in every cell but only differentiated cells of the pancreas glow.

13. 1)We learned we can make a reporter gene construct by fusing any gene promoter of interest to the GFP transcription unit. When fused to the insulin promoter, for example, the hybrid gene would be in every cell but only differentiated cells of the pancreas glow. 2)We can use the same construct and kind of flip it’s purpose. The insulin promoter is a “tissue-specific” promoter that let’s us tag a specific cell type.

14. migrating mouse germ line cells tagged with GFP

17. Developing kidney in a mouse strain tagged with GFP constructs.

18. migrating mouse germ line cells tagged with GFP

20. 9.6 germ cell migration

21. 9.20 SRY on Y chromosome

23. These are all somatic cells, including the cells that makes estrogen and testosterone. The germline cells make eggs or sperm in response to whether they are in a testes or ovary (nonautonomous).

24. 9.23 fly gynandromorph Compare sex determination in people (vertebrates) (nonautonomous regulated by steroid hormones) to sex determination in fruit flies (cell autonomous = each cell decides if it is an male or female).

25. 9.24 fly sex determination pathway

26. 9.25 sex lethal feedback

31. 9.21 mammal gonad development Every embryo starts with bipotential somatic gonads.

32. WRASSE

34. During germ line cell migration, cells erase much of their DNA methylation / imprinting / epigenome.

35. Figs 9.11/12 imprinting

36. Chapter 9: Reproduction The Saga of the Germ line cells Fertilization Sex determination Dosage compensation

37. In the nucleus of each of their cells, women have a Barr body. It is one of her two X chromosomes that has been packed away and isn’t used. dosage compensation

38. In the nucleus of each of their cells, women have a Barr body. It is one of her two X chromosomes that has been packed away and isn’t used. dosage compensation = Men have just one X and Women effectively have just one X, so the level of gene expression is the same in men and women.

39. 9.30 three pathways for dosage compensation

40. 9.33 three pathways for dosage compensation

41. 9.26 nematode sexes

42. Chapter 9: Reproduction The Saga of the Germ line cells Fertilization Sex determination Dosage compensation

43. 9.8 meiosis review

44. 9.9 gamete maturation

50. Chapter 9: Reproduction The Saga of the Germ line cells Fertilization Sex determination Dosage compensation

51. 9.14 fertilization

52. 9.15 egg cell membrane depolarization

53. 9.16 cortical granules

54. 9.17 intracellular Ca++ wave