1. LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson © 2011 Pearson Education, Inc. Lectures by Erin Barley Kathleen Fitzpatrick Animal Development Chapter 47

2. Overview: A Body-Building Plan A human embryo at about 7 weeks after conception shows development of distinctive features © 2011 Pearson Education, Inc.

3. Figure 47.1 1 mm

4. Development occurs at many points in the life cycle of an animal This includes metamorphosis and gamete production, as well as embryonic development © 2011 Pearson Education, Inc.

5. Figure 47.2 EMBRYONIC DEVELOPMENT Sperm Adult frog Egg Metamorphosis Larval stages Zygote Blastula Gastrula Tail-bud embryo FERTILIZATION CLEAVAGE GASTRULATION ORGANO- GENESIS

6. Although animals display different body plans, they share many basic mechanisms of development and use a common set of regulatory genes Biologists use model organisms to study development, chosen for the ease with which they can be studied in the laboratory © 2011 Pearson Education, Inc.

7. Concept 47.1: Fertilization and cleavage initiate embryonic development Fertilization is the formation of a diploid zygote from a haploid egg and sperm © 2011 Pearson Education, Inc.

8. Fertilization Molecules and events at the egg surface play a crucial role in each step of fertilization –Sperm penetrate the protective layer around the egg –Receptors on the egg surface bind to molecules on the sperm surface –Changes at the egg surface prevent polyspermy, the entry of multiple sperm nuclei into the egg © 2011 Pearson Education, Inc.

9. The Acrosomal Reaction The acrosomal reaction is triggered when the sperm meets the egg The acrosome at the tip of the sperm releases hydrolytic enzymes that digest material surrounding the egg © 2011 Pearson Education, Inc.

10. Figure 47.3-1 Basal body (centriole) Sperm head Acrosome Jelly coat Sperm-binding receptors Vitelline layer Egg plasma membrane

11. Figure 47.3-2 Basal body (centriole) Sperm head Acrosome Jelly coat Sperm-binding receptors Hydrolytic enzymes Vitelline layer Egg plasma membrane

12. Figure 47.3-3 Basal body (centriole) Sperm nucleus Sperm head Acrosome Jelly coat Sperm-binding receptors Hydrolytic enzymes Vitelline layer Egg plasma membrane Actin filament Acrosomal process

13. Figure 47.3-4 Basal body (centriole) Sperm plasma membrane Sperm nucleus Sperm head Acrosome Jelly coat Sperm-binding receptors Fused plasma membranes Hydrolytic enzymes Vitelline layer Egg plasma membrane Actin filament Acrosomal process

14. Figure 47.3-5 Basal body (centriole) Sperm plasma membrane Sperm nucleus Sperm head Acrosome Jelly coat Sperm-binding receptors Fertilization envelope Cortical granule Fused plasma membranes Hydrolytic enzymes Vitelline layer Egg plasma membrane Perivitelline space EGG CYTOPLASM Actin filament Acrosomal process

15. Gamete contact and/or fusion depolarizes the egg cell membrane and sets up a fast block to polyspermy © 2011 Pearson Education, Inc.

16. The Cortical Reaction Fusion of egg and sperm also initiates the cortical reaction Seconds after the sperm binds to the egg, vesicles just beneath the egg plasma membrane release their contents and form a fertilization envelope The fertilization envelope acts as the slow block to polyspermy © 2011 Pearson Education, Inc.

17. The cortical reaction requires a high concentration of Ca 2  ions in the egg The reaction is triggered by a change in Ca 2  concentration Ca 2  spread across the egg correlates with the appearance of the fertilization envelope © 2011 Pearson Education, Inc.

18. Figure 47.4 10 sec after fertilization 25 sec 35 sec 1 min 500  m 30 sec 20 sec 10 sec after fertilization 1 sec before fertilization Point of sperm nucleus entry Spreading wave of Ca 2  Fertilization envelope EXPERIMENT RESULTS CONCLUSION

19. Egg Activation The rise in Ca 2+ in the cytosol increases the rates of cellular respiration and protein synthesis by the egg cell With these rapid changes in metabolism, the egg is said to be activated The proteins and mRNAs needed for activation are already present in the egg The sperm nucleus merges with the egg nucleus and cell division begins © 2011 Pearson Education, Inc.

20. Fertilization in Mammals Fertilization in mammals and other terrestrial animals is internal Secretions in the mammalian female reproductive tract alter sperm motility and structure © 2011 Pearson Education, Inc.

21. Sperm travel through an outer layer of cells to reach the zona pellucida, the extracellular matrix of the egg When the sperm binds a receptor in the zona pellucida, it triggers a slow block to polyspermy No fast block to polyspermy has been identified in mammals © 2011 Pearson Education, Inc.

22. Figure 47.5 Zona pellucida Follicle cell Sperm basal body Sperm nucleus Cortical granules

23. In mammals the first cell division occurs 12  36 hours after sperm binding The diploid nucleus forms after this first division of the zygote © 2011 Pearson Education, Inc.

24. Cleavage Fertilization is followed by cleavage, a period of rapid cell division without growth Cleavage partitions the cytoplasm of one large cell into many smaller cells called blastomeres The blastula is a ball of cells with a fluid-filled cavity called a blastocoel © 2011 Pearson Education, Inc.

25. Figure 47.6 (a) Fertilized egg (b) Four-cell stage (c) Early blastula (d) Later blastula 50  m

26. In frogs and many other animals, the distribution of yolk (stored nutrients) is a key factor influencing the pattern of cleavage The vegetal pole has more yolk; the animal pole has less yolk The difference in yolk distribution results in animal and vegetal hemispheres that differ in appearance © 2011 Pearson Education, Inc. Cleavage Patterns

27. The first two cleavage furrows in the frog form four equally sized blastomeres The third cleavage is asymmetric, forming unequally sized blastomeres © 2011 Pearson Education, Inc.

28. Holoblastic cleavage, complete division of the egg, occurs in species whose eggs have little or moderate amounts of yolk, such as sea urchins and frogs Meroblastic cleavage, incomplete division of the egg, occurs in species with yolk-rich eggs, such as reptiles and birds © 2011 Pearson Education, Inc.

29. Zygote 2-cell stage forming 4-cell stage forming 8-cell stage Vegetal pole Blastula (cross section) Gray crescent Animal pole Blastocoel 0.25 mm 8-cell stage (viewed from the animal pole) Blastula (at least 128 cells) Figure 47.7

30. Figure 47.7a-1 Zygote

31. Figure 47.7a-2 Zygote 2-cell stage forming Gray crescent

32. Figure 47.7a-3 Zygote 2-cell stage forming 4-cell stage forming Gray crescent

33. Figure 47.7a-4 Zygote 2-cell stage forming 4-cell stage forming 8-cell stage Vegetal pole Gray crescent Animal pole

34. Figure 47.7a-5 Zygote 2-cell stage forming 4-cell stage forming 8-cell stage Vegetal pole Blastula (cross section) Gray crescent Animal poleBlastocoel

35. Figure 47.7b Animal pole 0.25 mm 8-cell stage (viewed from the animal pole)

36. Figure 47.7c 0.25 mm Blastocoel Blastula (at least 128 cells)

37. Animal embryos complete cleavage when the ratio of material in the nucleus relative to the cytoplasm is sufficiently large © 2011 Pearson Education, Inc. Regulation of Cleavage

38. After cleavage, the rate of cell division slows and the normal cell cycle is restored Morphogenesis, the process by which cells occupy their appropriate locations, involves –Gastrulation, the movement of cells from the blastula surface to the interior of the embryo –Organogenesis, the formation of organs © 2011 Pearson Education, Inc. Concept 47.2: Morphogenesis in animals involves specific changes in cell shape, position, and survival

39. Gastrulation Gastrulation rearranges the cells of a blastula into a three-layered embryo, called a gastrula © 2011 Pearson Education, Inc.

40. The three layers produced by gastrulation are called embryonic germ layers –The ectoderm forms the outer layer –The endoderm lines the digestive tract –The mesoderm partly fills the space between the endoderm and ectoderm Each germ layer contributes to specific structures in the adult animal © 2011 Pearson Education, Inc. Video: Sea Urchin Embryonic Development

41. ECTODERM (outer layer of embryo) MESODERM (middle layer of embryo) ENDODERM (inner layer of embryo) Epidermis of skin and its derivatives (including sweat glands, hair follicles) Epithelial lining of digestive tract and associated organs (liver, pancreas) Epithelial lining of respiratory, excretory, and reproductive tracts and ducts Germ cells Jaws and teeth Pituitary gland, adrenal medulla Nervous and sensory systems Skeletal and muscular systems Circulatory and lymphatic systems Excretory and reproductive systems (except germ cells) Dermis of skin Adrenal cortex Thymus, thyroid, and parathyroid glands Figure 47.8

42. Gastrulation begins at the vegetal pole of the blastula Mesenchyme cells migrate into the blastocoel The vegetal plate forms from the remaining cells of the vegetal pole and buckles inward through invagination © 2011 Pearson Education, Inc. Gastrulation in Sea Urchins

43. The newly formed cavity is called the archenteron This opens through the blastopore, which will become the anus © 2011 Pearson Education, Inc.

44. Animal pole Blastocoel Mesenchyme cells Vegetal plate Vegetal pole Blastocoel Filopodia Mesenchyme cells Blastopore Archenteron 50  m Ectoderm Mouth Mesenchyme (mesoderm forms future skeleton) Blastopore Blastocoel Archenteron Digestive tube (endoderm) Anus (from blastopore) Key Future ectoderm Future mesoderm Future endoderm Figure 47.9

45. Figure 47.9a-5 Key Animal pole Blastocoel Mesenchyme cells Vegetal plate Vegetal pole Archenteron Filopodia Archenteron Blastocoel Blastopore Mouth Mesenchyme (mesoderm forms future skeleton) Anus (from blastopore) Digestive tube (endoderm) Ectoderm Future ectoderm Future mesoderm Future endoderm

46. Frog gastrulation begins when a group of cells on the dorsal side of the blastula begins to invaginate This forms a crease along the region where the gray crescent formed The part above the crease is called the dorsal lip of the blastopore © 2011 Pearson Education, Inc. Gastrulation in Frogs

47. Cells continue to move from the embryo surface into the embryo by involution These cells become the endoderm and mesoderm Cells on the embryo surface will form the ectoderm © 2011 Pearson Education, Inc.

48. Key Future ectoderm Future mesoderm Future endoderm SURFACE VIEW CROSS SECTION Animal pole Vegetal pole Early gastrula Blastocoel Dorsal lip of blasto- pore Blastopore Dorsal lip of blastopore Blastocoel shrinking Archenteron Blastocoel remnant Ectoderm Mesoderm Endoderm Blastopore Yolk plug Blastopore Late gastrula 321 Figure 47.10

49. Prior to gastrulation, the embryo is composed of an upper and lower layer, the epiblast and hypoblast, respectively During gastrulation, epiblast cells move toward the midline of the blastoderm and then into the embryo toward the yolk © 2011 Pearson Education, Inc. Gastrulation in Chicks

50. The midline thickens and is called the primitive streak The hypoblast cells contribute to the sac that surrounds the yolk and a connection between the yolk and the embryo, but do not contribute to the embryo itself © 2011 Pearson Education, Inc.

51. Future ectoderm Migrating cells (mesoderm) Blastocoel Epiblast YOLK Endoderm Hypoblast Primitive streak Fertilized egg Primitive streak Embryo Yolk Figure 47.11

52. Human eggs have very little yolk A blastocyst is the human equivalent of the blastula The inner cell mass is a cluster of cells at one end of the blastocyst The trophoblast is the outer epithelial layer of the blastocyst and does not contribute to the embryo, but instead initiates implantation © 2011 Pearson Education, Inc. Gastrulation in Humans

53. Following implantation, the trophoblast continues to expand and a set of extraembryonic membranes is formed These enclose specialized structures outside of the embryo Gastrulation involves the inward movement from the epiblast, through a primitive streak, similar to the chick embryo © 2011 Pearson Education, Inc.

54. Blastocyst reaches uterus. 1234 Blastocyst implants (7 days after fertilization). Extraembryonic membranes start to form (10–11 days), and gastrulation begins (13 days). Gastrulation has produced a three-layered embryo with four extraembryonic membranes. Uterus Maternal blood vessel Endometrial epithelium (uterine lining) Inner cell mass Trophoblast Blastocoel Expanding region of trophoblast Epiblast Hypoblast Trophoblast Expanding region of trophoblast Amniotic cavity Epiblast Hypoblast Yolk sac (from hypoblast) Extraembryonic mesoderm cells (from epiblast) Chorion (from trophoblast) Amnion Chorion Ectoderm Mesoderm Endoderm Yolk sac Extraembryonic mesoderm Allantois Figure 47.12

55. Organogenesis During organogenesis, various regions of the germ layers develop into rudimentary organs Early in vertebrate organogenesis, the notochord forms from mesoderm, and the neural plate forms from ectoderm © 2011 Pearson Education, Inc.

56. Figure 47.13 Neural folds 1 mm Neural fold Neural plate Notochord Ectoderm Mesoderm Endoderm Archenteron (a) Neural plate formation (b) Neural tube formation (c) Somites Neural fold Neural plate Neural crest cells Outer layer of ectoderm Neural crest cells Neural tube Eye SomitesTail bud SEM Neural tube Notochord Coelom Neural crest cells Somite Archenteron (digestive cavity) 1 mm

57. Figure 47.13a Neural folds 1 mm Neural fold Neural plate Notochord Ectoderm Mesoderm Endoderm Archenteron (a) Neural plate formation

58. The neural plate soon curves inward, forming the neural tube The neural tube will become the central nervous system (brain and spinal cord) © 2011 Pearson Education, Inc. Video: Frog Embryo Development

59. (b) Neural tube formation Neural fold Neural plate Neural crest cells Outer layer of ectoderm Neural crest cells Neural tube Figure 47.13b-3

60. Neural crest cells develop along the neural tube of vertebrates and form various parts of the embryo (nerves, parts of teeth, skull bones, and so on) Mesoderm lateral to the notochord forms blocks called somites Lateral to the somites, the mesoderm splits to form the coelom (body cavity) © 2011 Pearson Education, Inc.

61. Figure 47.13c (c) Somites Eye Somites Tail bud SEM Neural tube Notochord Coelom Neural crest cells Somite Archenteron (digestive cavity) 1 mm

62. Mechanisms of Morphogenesis Morphogenesis in animals but not plants involves movement of cells © 2011 Pearson Education, Inc.

63. Reorganization of the cytoskeleton is a major force in changing cell shape during development For example, in neurulation, microtubules oriented from dorsal to ventral in a sheet of ectodermal cells help lengthen the cells along that axis © 2011 Pearson Education, Inc. The Cytoskeleton in Morphogenesis

64. Figure 47.15-1 Ectoderm

65. Figure 47.15-2 Ectoderm Neural plate Microtubules

66. Figure 47.15-3 Ectoderm Neural plate Microtubules Actin filaments

67. Figure 47.15-4 Ectoderm Neural plate Microtubules Actin filaments

68. Figure 47.15-5 Ectoderm Neural plate Microtubules Actin filaments Neural tube

69. The cytoskeleton promotes elongation of the archenteron in the sea urchin embryo This is convergent extension, the rearrangement of cells of a tissue that cause it to become narrower (converge) and longer (extend) Convergent extension occurs in other developmental processes The cytoskeleton also directs cell migration © 2011 Pearson Education, Inc.

70. Figure 47.16 Extension Convergence

71. Programmed cell death is also called apoptosis At various times during development, individual cells, sets of cells, or whole tissues stop developing and are engulfed by neighboring cells For example, many more neurons are produced in developing embryos than will be needed Extra neurons are removed by apoptosis © 2011 Pearson Education, Inc. Programmed Cell Death

72. Concept 47.3: Cytoplasmic determinants and inductive signals contribute to cell fate specification Determination is the term used to describe the process by which a cell or group of cells becomes committed to a particular fate Differentiation refers to the resulting specialization in structure and function © 2011 Pearson Education, Inc.

73. Cells in a multicellular organism share the same genome Differences in cell types is the result of the expression of different sets of genes © 2011 Pearson Education, Inc.

74. Fate Mapping Fate maps are diagrams showing organs and other structures that arise from each region of an embryo Classic studies using frogs indicated that cell lineage in germ layers is traceable to blastula cells © 2011 Pearson Education, Inc.

75. Epidermis Central nervous system Notochord Mesoderm Endoderm BlastulaNeural tube stage (transverse section) (a) Fate map of a frog embryo 64-cell embryos Blastomeres injected with dye Larvae (b) Cell lineage analysis in a tunicate Figure 47.17

76. Figure 47.17a Epidermis Central nervous system Notochord Mesoderm Endoderm Blastula Neural tube stage (transverse section) (a) Fate map of a frog embryo

77. Figure 47.17b 64-cell embryos Blastomeres injected with dye Larvae (b) Cell lineage analysis in a tunicate

78. Axis Formation A body plan with bilateral symmetry is found across a range of animals This body plan exhibits asymmetry across the dorsal-ventral and anterior-posterior axes The right-left axis is largely symmetrical © 2011 Pearson Education, Inc.

79. The anterior-posterior axis of the frog embryo is determined during oogenesis The animal-vegetal asymmetry indicates where the anterior-posterior axis forms The dorsal-ventral axis is not determined until fertilization © 2011 Pearson Education, Inc.

80. Upon fusion of the egg and sperm, the egg surface rotates with respect to the inner cytoplasm This cortical rotation brings molecules from one area of the inner cytoplasm of the animal hemisphere to interact with molecules in the vegetal cortex This leads to expression of dorsal- and ventral- specific gene expression © 2011 Pearson Education, Inc.

81. Dorsal Right AnteriorPosterior Ventral Left (a) The three axes of the fully developed embryo (b) Establishing the axes Animal hemisphere Vegetal hemisphere Animal pole Vegetal pole Point of sperm nucleus entry Gray crescent Pigmented cortex Future dorsal side First cleavage Figure 47.21

82. In chicks, gravity is involved in establishing the anterior-posterior axis Later, pH differences between the two sides of the blastoderm establish the dorsal-ventral axis In mammals, experiments suggest that orientation of the egg and sperm nuclei before fusion may help establish embryonic axes © 2011 Pearson Education, Inc.

83. Control egg (dorsal view) 1a1b Gray crescent Control group Experimental group Experimental egg (side view) Gray crescent EXPERIMENT Thread Figure 47.22-1

84. Control egg (dorsal view) 2 1a1b Gray crescent Control group Experimental group Experimental egg (side view) Gray crescent Thread Normal Belly piece EXPERIMENT RESULTS Figure 47.22-2

85. In mammals, embryonic cells remain totipotent until the 8-cell stage, much longer than other organisms Progressive restriction of developmental potential is a general feature of development in all animals In general tissue-specific fates of cells are fixed by the late gastrula stage © 2011 Pearson Education, Inc.

86. Cell Fate Determination and Pattern Formation by Inductive Signals As embryonic cells acquire distinct fates, they influence each other’s fates by induction © 2011 Pearson Education, Inc.

87. The “Organizer” of Spemann and Mangold Spemann and Mangold transplanted tissues between early gastrulas and found that the transplanted dorsal lip triggered a second gastrulation in the host The dorsal lip functions as an organizer of the embryo body plan, inducing changes in surrounding tissues to form notochord, neural tube, and so on © 2011 Pearson Education, Inc.

88. Figure 47.23 Dorsal lip of blastopore Pigmented gastrula (donor embryo) Nonpigmented gastrula (recipient embryo) Primary embryo Secondary (induced) embryo Primary structures: Neural tube Notochord Secondary structures: Notochord (pigmented cells) Neural tube (mostly nonpigmented cells) EXPERIMENT RESULTS

89. Formation of the Vertebrate Limb Inductive signals play a major role in pattern formation, development of spatial organization The molecular cues that control pattern formation are called positional information This information tells a cell where it is with respect to the body axes It determines how the cell and its descendents respond to future molecular signals © 2011 Pearson Education, Inc.

90. The wings and legs of chicks, like all vertebrate limbs, begin as bumps of tissue called limb buds © 2011 Pearson Education, Inc.

91. Figure 47.24 Limb buds 50  m Anterior Limb bud AER ZPA Posterior Apical ectodermal ridge (AER) (a) Organizer regions (b) Wing of chick embryo Digits Anterior Proximal Dorsal Posterior Ventral Distal 2 3 4

92. Figure 47.24a Limb buds 50  m Anterior Limb bud AER ZPA Posterior Apical ectodermal ridge (AER) (a) Organizer regions

93. The embryonic cells in a limb bud respond to positional information indicating location along three axes –Proximal-distal axis –Anterior-posterior axis –Dorsal-ventral axis © 2011 Pearson Education, Inc.

94. One limb-bud regulating region is the apical ectodermal ridge (AER) The AER is thickened ectoderm at the bud’s tip The second region is the zone of polarizing activity (ZPA) The ZPA is mesodermal tissue under the ectoderm where the posterior side of the bud is attached to the body © 2011 Pearson Education, Inc.

95. Tissue transplantation experiments support the hypothesis that the ZPA produces an inductive signal that conveys positional information indicating “posterior” © 2011 Pearson Education, Inc.

96. Figure 47.25 Donor limb bud Host limb bud ZPA Anterior Posterior New ZPA 4 4 3 3 2 2 EXPERIMENT RESULTS