1. YEAST LAB DEAD YEAST W/MINERAL OIL
2 mL of sucrose solution to 50 mL beaker
Add 2 mL of DEAD yeast; pipette up and down 2-3x to mix
Cover this solution with mineral oil
Incubate beaker in warm water bath (38°-42°C) for 5 min
Place both beakers into biochamber, insert probe and press collect (data should collect every 15 seconds for 4 minutes)
After data collection, restore warm water bath temperature
Fan air over bio-chamber to restore gas levels

2. YEAST LAB DEAD YEAST W/O MINERAL OIL
Pipette the yeast solution from under mineral oil to clean 50 mL beaker (better to transfer less yeast than to transfer mineral oil)
Incubate beaker in warm water bath (38°-42°C) for 5 min
Place both beakers into biochamber, insert probe and press collect
When asked, choose ‘store latest run’
Data should collect every 15 seconds for 4 minutes

3. YEAST LAB LIVE YEAST W/MINERAL OIL
2 mL of sucrose solution to 50 mL beaker
Add 2 mL of LIVE yeast; pipette up and down 2-3x to mix
*Be careful, pipetting too vigorously will kill the yeast
Cover this solution with mineral oil
Incubate beaker in warm water bath (38°-42°C) for 5 min
Place both beakers into biochamber, insert probe and press collect, click ‘store latest run’ (data should collect every 15 seconds for 4 minutes)
After data collection, restore warm water bath temperature
Fan air over bio-chamber to restore gas levels

4. YEAST LAB LIVE YEAST W/O MINERAL OIL
Pipette the yeast solution from under mineral oil to clean 50 mL beaker (better to transfer less yeast than to transfer mineral oil).
Incubate beaker in warm water bath (38°-42°C) for 5 min
Place both beakers into biochamber, insert probe and press collect (data should collect every 15 seconds for 4 minutes)
When asked, choose ‘store latest run’