1. Lesson Objectives Describe the equipment needed for microscopy and explain how each piece is used. Calculate the linear magnification of drawings and the actual size of specimens in images of known magnification.

2. Image from wikimedia commons: http://commons.wikimedia.org/wiki/File:Microscope-blank.svghttp://commons.wikimedia.org/wiki/File:Microscope-blank.svg Magnification The image we see through the light microscope has been magnified. Objective lens x eyepiece lens

3. Modern Microscopy Image: d2540-6 by USDA on Flickr (CC): http://flic.kr/p/dPqvvYhttp://flic.kr/p/dPqvvY

4. Modern Microscopy Image: d2540-6 by USDA on Flickr (CC): http://flic.kr/p/dPqvvYhttp://flic.kr/p/dPqvvY As we develop more and more sophisticated and precise imaging tools, we can see more detail of the cells and molecules that make us. Scanning electron microscopes deliver high- resolution, 3D surface images of structures, whereas transmission electron microscopes give us a view inside cells and organelles.

5. Emiliana huxleyi Image from: http://earthguide.ucsd.edu/earthguide/imagelibrary/emilianiahuxleyi.htmlhttp://earthguide.ucsd.edu/earthguide/imagelibrary/emilianiahuxleyi.html

6. Transmission electron micrograph of HIV particles. HIV-1. Transmission electron micrograph, via wikimedia commons: http://commons.wikimedia.org/wiki/File%3AHIV- 1_Transmission_electron_micrograph_AIDS02bbb_lores.jpghttp://commons.wikimedia.org/wiki/File%3AHIV- 1_Transmission_electron_micrograph_AIDS02bbb_lores.jpg 120nm

7. False-coloured scanning electron micrograph of HIV (green) budding on a lymphicoyte (blue) http://en.wikipedia.org/wiki/File:HIV-budding-Color.jpghttp://en.wikipedia.org/wiki/File:HIV-budding-Color.jpg OR http://phil.cdc.gov/phil/details.asp?pid=10000http://phil.cdc.gov/phil/details.asp?pid=10000 1μm Scanning electron micrograph of HIV particles budding on a human lymphocyte.

8. Some units that I use & know ÷1,000 X 1,000 μm = micrometers We usually use this in discussion of cells. There are 1,000μm in one mm. write this correctly

9. Here is an image from a microscope. How do you know how big it really is? Any ideas what it is? Magnification x150

10. Lets practice a couple of questions (worksheet 1) Now lets play with the microscopes!

11. The next few slides show some worked examples to help you with future calculations

16. Extension / Plenary Try questions 1 – 3 (all parts) in the text book pages 6-7

17. Labeling the parts of the microscope activity: http://www.biology.ualberta.ca/facilities/multimedia/uploads/intro-biology/microscope.html