1. CLEANSED Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and road building LIFE12ENV /IT/000652 Francesca Ugolini, Francesca Martelli, Francesco Sabatini, Luciano Massetti Institute of Biometeorology, Firenze September 10 th, 2015. Murcia

2. Growth monitoring through height measurement and pictures followed by elaboration of growth curve Physiological responses (leaf gas exchanges & photosystem efficiency, leaf water potentials) Soil respiration Soil humidity in continuum and in vertical transect Meteorological data analysis LIST OF MONITORING ACTIONS May-September (ongoing) 2015 September 10 th, 2015. Murcia

3. Climate data September 10 th, 2015. Murcia

4. Growth curve Plants were pruned at the end of January 2015 Measure of the longest branch for each plant between June and September 2015 Average height for each species and each thesis Relationship between plant growth data and Growing Degree Days (GDD) °C d day of the year DOY (k=1, 1° January; k=90, 31° March ) T k Average air temperature day k if T k >0 else 0 September 10 th, 2015. Murcia

5. Height measurements a b aa b a a b ba b b a b ca b c a b ba b b a b ba b b a b ba b b Significant differences between treatment within the same species by ANOVA followed by Bonferroni test at p < 0.05, indicated by small letters.

6. Growth September 10 th, 2015. Murcia 30 Jan 20158 Sept 2015

7. Growth curve September 10 th, 2015. Murcia

8. Viburnum tinus September 10 th, 2015. Murcia CTL T33 T50

9. Growth curve SpeciesT0T33T50 V.tinus1.612.122.23 E. macrophylla6.857.097.03 P.x fraseri6.066.315.66 Growth rate: cm / (100 GDD) September 10 th, 2015. Murcia

10. PHYSIOLOGICAL RESPONSES Eleagnus macrophylla L.Photinia x fraseri var. Red RobinViburnum tinus L. Evapotraspiration: sporadically higher in T50 Evapotraspiration: more frequently higher in T50 Photosynthesis & Stomatal conductance: similar trend between the substrates; sporadically higher in CTL Photosynthesis & Stomatal conductance: similar trend between the substrates Photosynthesis & Stomatal conductance: more frequently higher in T50

11. September 10 th, 2015. Murcia PHYSIOLOGICAL RESPONSES Pre-dawn potentials (4-5 a.m.) The addition of phytoremediated sediments has effects only in Eleagnus and Viburnum. Eleagnus changes response along the summer with better water state in presence of phytoremediated sediments Viburnum has lower water potentials, especially in presence of phytoremediated sediments (T33)

12. PHYSIOLOGICAL RESPONSES Minimum Potentials (1-2 p.m.) At the time of the maximum water stress, Photinia and Viburnum have shown different responses in presence of phytoremediated sediments. Viburnum reached the lowest Ψmin in T33 (in August) whereas in T50 and CTL values were similar; Photinia also had lowest values in T33 (July and August) but inconsistencies are found in T50 and CTL. Photinia and Viburnum tend to show a water stress condition in T33 September 10 th, 2015. Murcia

13. SOIL RESPIRATION The addition of phytoremediated sediments increases soil respiration (more aeration, microbiological activity, root development ?) September 10 th, 2015. Murcia

14. LEAF CHEMICAL ANALYSIS (C. Garcia Izquierdo) Microelements and heavy metals analysis (main results) In Red: results different from year 2014 N CCTL & T33 > T50 in Eleagnus (range: 44.3-45.5 g/100g) NaCTL & T50 > T33 in Eleagnus & Viburnum & Photinia (range: 0.015-0.005 g/100g) PCTL > T33 & T50 in Viburnum & Photinia (range: 0.12-0.08 g/100g) Mo T50 & T33 > CTL in Viburnum & Photinia (range: 0.4-1.3 mg/Kg) N.S. in Eleagnus (range: ~100 mg/Kg) Cd T50 & T33 > CTL (tracce) in Viburnum & Photinia (range in Photinia: 0.4-0.6 mg/Kg; range in Viburnum: 3.2-5 mg/Kg). Tracce in Eleagnus Pb T33 > CTL in Photinia (range: <0.1-0.1 mg/Kg) ; T50 >T33 in Viburnum (range: 0.08-0.18 mg/Kg); N.S. in Eleagnus (range: 2.6-3 mg/Kg) Ni T50 & T33 > CTL in Eleagnus (range: 2.3-22.1 mg/Kg); Range in Viburnum: 0.48-0.6 mg/Kg; tracce in Photinia Sr T50 > CTL in Photinia & Viburnum (range: 28-44 mg/Kg) N.S in Eleagnus (tracce) Zn T50 > CTL in Photinia (range: 11-15 mg/Kg) T50 >T33 > CTL in Viburnum (range 21-34 mg/Kg) CTL > T50 in Eleagnus (range: 1-1.4 mg/Kg) September 10 th, 2015. Murcia

15. Eleagnus macrophylla L. CTLT33T50 N (g/100g)2.51±0.312.46±0.282.69±0.39 C (g/100g)45.57±0.59 a45.81±0.82 a44.28 ±0.61 b Al (mg/kg)54.47±19.0747.84±29.3847.06±9.01 Ca (g/100g)0.90±0.110.77±0.250.90±0.16 Mg (g/100g)0.12±0.020.12±0.040.14±0.03 Fe (mg/kg)52.57±6.79 c56.93±13.30 ab68.99±13.84 a K (g/100g)1.63±0.261.51±0.311.68±0.23 Na (g/100g)0.0076±0.002 a0.0041±0.0008 b0.0076±0.004 a P (g/100g)0.12±0.010.14±0.030.14±0.02 S (g/100g)0.15±0.030.16±0.030.17±0.02 As (mg/Kg)<0.1 Be (mg/Kg)<0.5 Bi (mg/Kg)<0.5 B (mg/Kg)33.90±4.2826.75±8.8226.71±5.11 Cd (mg/Kg)<0.1 Co (mg/Kg)54.47±19.0747.84±29.3847.06±9.01 Cr (mg/kg)<0.5 Cu (mg/kg)0.12±0.10<0.10.11±0.16 Li (mg/kg)13.62±4.2515.81±5.9714.39±3.42 Mn (mg/kg)0.80±0.570.45±0.220.61±0.21 Mo (mg/kg)116.65±23.4496.64±35.51109.95±26.94 Ni (mg/kg)2.29±1.48 b17.34±7.86 a22.12±9.17 a Pb (mg/kg)2.80±0.842.60±0.843.01±0.75 Sb (mg/kg)0.12±0.100.13±0.100.09±0.04 Se (mg/kg)<0.5 Sr (mg/kg)<0.1 Ti (mg/kg)22.35±3.5523.97±8.1230.95±6.49 Tl (mg/kg)1.4±0.421.08±0.41.07±0.22 V (mg/Kg)<0.5 Zn (mg/kg)1.41±0.42 a1.08±0.40 ab1.08±0.22 b

16. Viburnum tinus L. CTLT33T50 N (g/100g) 1.44 ±0.07 1.37±0.091.46±0.12 C (g/100g)48.2±1.248.1±0.748±0.7 Al (mg/kg)91.48±25.069.57±25.493.88±31.0 Ca (g/100g)0.62±0.0790.62±0.0640.69±0.060 Mg (g/100g)0.20±0.0260.18±0.020.19±0.016 Fe (mg/kg)65.58±14.3952.71±15.2466.83±16.47 K (g/100g)1.36±0.171.31±0.071.36±0.08 Na (g/100g)0.0141±0.0029 a0.0040±0.0008 b0.0153±0.0049 a P (g/100g)0.12±0.012 a0.10±0.013 b0.11±0.012 b S (g/100g)0.108±0.010 ab0.099±0.008 b0.111±0.006 a As (mg/Kg)<0.1 Be (mg/Kg)<0.5 Bi (mg/Kg) <0.5 B (mg/Kg) 24.42±2.90 b26.54±2.68 ab30.36±4.34 a Cd (mg/Kg) <0.1 b3.22±0.94 a4.97±1.10 a Co (mg/Kg) <0.5 Cr (mg/kg) 37.99±52.1875.79±46.6963.20±52.17 Cu (mg/kg) 9.54±5.706.77±1.658.77±3.82 Li (mg/kg) 0.43±0.170.40±0.080.51±0.09 Mn (mg/kg) 50.26±14.30 a26.72±6.50 b27.26±5.83 b Mo (mg/kg) 0.44±0.19 b0.89±0.25 a0.78±0.22 a Ni (mg/kg) 0.48±0.220.57±0.160.60±0.14 Pb (mg/kg) 0.13±0.08 ab0.08±0.04 b0.18±0.10 a Sb (mg/kg) <0.5 Se (mg/kg) <0.1 Sr (mg/kg) 27.81±4.89 b31.50±4.04 ab35.68±4.38 a Ti (mg/kg)2.42±0.592.05±0.662.75±0.90 Tl (mg/Kg)2.76±1.591.4±1.21.84±0.82 V (mg/kg)<0.5 Zn (mg/kg)21.06±2.76 c26.89±2.55 b34.51±2.09 a September 10 th, 2015. Murcia

17. Photinia x fraseri CTLT33T50 N (g/100g)0.97±0.070.99±0.10.98±0.06 C (g/100g)46.3±0.5746.4±0.946.2±0.7 Al (mg/kg)55.84±8.8458.22±10.3064.91±23.54 Ca (g/100g)1.02±0.151.08±0.261.06±0.21 Mg (g/100g)0.19±0.0180.17±0.0150.17±0.019 Fe (mg/kg)30.64±3.4533.97±5.1632.98±5.79 K (g/100g)1.23±0.1761.12±0.0791.22±0.213 Na (g/100g)0.01±0.003 a0.005±0.002 b0.011±0.005 a P (g/100g)0.12±0.04 a0.08±0.009 b0.08±0.022 b S (g/100g)0.048±0.0070.049±0.006 As (mg/Kg)<0.1 Be (mg/Kg)<0.5 Bi (mg/Kg) <0.5 B (mg/Kg) 24.17±4.6122.85±2.6829.61±8.62 Cd (mg/Kg) <0.1 c0.40±0.09 b0.56±0.16 a Co (mg/Kg) <0.5 Cr (mg/kg) <0.10.13±0.21<0.1 Cu (mg/kg) 4.34±1.106.12±1.694.66±2.29 Li (mg/kg) 0.68±0.110.91±0.350.73±0.23 Mn (mg/kg) 16.01±0.89 a12.61±1.39 b11.46±0.86 b Mo (mg/kg) 0.41±0.18 b0.97±0.31 a1.28±0.45 a Ni (mg/kg) <0.1 Pb (mg/kg) <0.1 b0.1±0.06 a<0.1 ab Sb (mg/kg) <0.5 Se (mg/kg) <0.1 Sr (mg/kg) 30.46±4.41 b39.86±10.32 ab43.61±11.43 a Ti (mg/kg)1.24±0.241.52±0.281.33±0.44 Tl (mg/kg)3.18±1.083.21±1.412.55±1.49 V (mg/kg)<0.5 Zn (mg/kg)11.22±2.25 b13.80±1.50 ab14.78±2.65 a

18. Pending tasks (Actions C) Measurement campaign still ongoing Missing analysis: Water Use Efficiency Photosystem efficiency Chlorophyll content Overall data revision September 10 th, 2015. Murcia

19. IBIMET Budget situation September 10 th, 2015. Murcia Mid Term Report Estimated costs after midtermtotal costProject BudgetAmendmentDifference Personnel 114893.71 *35000149893.7114630019280046500 Travel 2541.1 40006541.112500 0 External assistance 14035 26000400357372061720-12000 consumables 5688.4 40009688.45450020000-34500 *Cost of temporary contract, it doesn’t include permament staff costs

20. Next tasks and point of discussion Guidelines for the use of sediments in plant nursery: template? Video Final meeting promoted by Tuscany? Papers, review … September 10 th, 2015. Murcia