1. Lesson 5 Using Crossbreeding and Hybrids

2. Next Generation Science/Common Core Standards Addressed! HS ‐ LS1 ‐ 1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells. [Assessment Boundary: Assessment does not include identification of specific cell or tissue types, whole body systems, specific protein structures and functions, or the biochemistry of protein synthesis.] HS ‐ LS1 ‐ 1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells. [Assessment Boundary: Assessment does not include identification of specific cell or tissue types, whole body systems, specific protein structures and functions, or the biochemistry of protein synthesis.]

3. Agriculture, Food and Natural Resource Standards Addressed! PS.03.01. Demonstrate plant propagation techniques in plant system activities. PS.03.01. Demonstrate plant propagation techniques in plant system activities. PS.03.01.03.a. Summarize optimal conditions for asexual propagation and demonstrate techniques used to propagate plants by cuttings, division, separation, layering, budding and grafting. PS.03.01.03.a. Summarize optimal conditions for asexual propagation and demonstrate techniques used to propagate plants by cuttings, division, separation, layering, budding and grafting.

4. Bell Work Define Plant Breeding Define Plant Breeding Define the processes of selection and hybridization Define the processes of selection and hybridization Explain genetic engineering in plants Explain genetic engineering in plants

5. Terms Agrobacterium Tumefacians Agrobacterium Tumefacians Ballistic Gene Transfer Ballistic Gene Transfer Cultivar Cultivar Genetic Engineering Genetic Engineering Genotype Genotype Hybrid vigor Hybrid vigor Hybridization Hybridization Microprojectiles Microprojectiles Mutation Mutation Phenotype Phenotype Plant Breeding Plant Breeding Selection Selection Totipotent Totipotent Transgenic plants Transgenic plants

6. Interest Approach Ask the students to tell you what they have heard about genetically modified organisms (GMO). Make a list of these items on the board. Discuss these items briefly. Ask the students to tell you what they have heard about genetically modified organisms (GMO). Make a list of these items on the board. Discuss these items briefly. Ask the students to make a copy of this list. Tell them that you will take another look at the items on this list after you have completed the unit. Ask the students to make a copy of this list. Tell them that you will take another look at the items on this list after you have completed the unit. At that time, ask what the students know about GMO’s now. What items changed? At that time, ask what the students know about GMO’s now. What items changed?

7. What is plant breeding? Plant breeding is the systematic process of improving plants using scientific methods. Plant breeding is the systematic process of improving plants using scientific methods. There are a variety of methods used to make certain plants are pollinated, as well as methods to prevent unwanted pollination of plants. There are a variety of methods used to make certain plants are pollinated, as well as methods to prevent unwanted pollination of plants.

8. The goals of plant breeding vary according to the type of improvement in the plant that is desired by the plant breeder. Examples; The goals of plant breeding vary according to the type of improvement in the plant that is desired by the plant breeder. Examples; Gain disease resistance Gain disease resistance Gain insect resistance Gain insect resistance Improve environmental adaptation Improve environmental adaptation Improve productivity Improve productivity Make a species more suited to cultural practices Make a species more suited to cultural practices Obtain a more desirable product from plants Obtain a more desirable product from plants

9. The genotype is the genetic makeup of a plant The genotype is the genetic makeup of a plant Plant breeders are continually looking for new plant genotypes that will produce desired results Plant breeders are continually looking for new plant genotypes that will produce desired results

10. Phenotype Phenotype is the outward or physical appearance of a plant Changes in genotype often result in a change in phenotype also.

11. Plant breeders often develop new plant cultivars Plant breeders often develop new plant cultivars A cultivar is a cultivated plant that has specific and distinguishable characteristics. A cultivar is a cultivated plant that has specific and distinguishable characteristics. Plants within a cultivar will retain the characteristics of that group when reproduced. Plants within a cultivar will retain the characteristics of that group when reproduced.

12. What are selection and hybridization? Selection is process of breeding plants that are selected for a particular characteristic. Selection is process of breeding plants that are selected for a particular characteristic. Selection leads to dominance of certain genetic traits Selection leads to dominance of certain genetic traits A mutation is a genetic variation that naturally occurs in the plant A mutation is a genetic variation that naturally occurs in the plant Mutations cannot be predicted Mutations cannot be predicted

13. Hybridization is the process of breeding individuals from distinctly different varieties Hybridization is the process of breeding individuals from distinctly different varieties Goal is gain a superior characteristic in the offspring. Goal is gain a superior characteristic in the offspring. Hybrid vigor is a condition where the offspring may have a greater yield, height, disease resistance, or other traits than either of the parents. Hybrid vigor is a condition where the offspring may have a greater yield, height, disease resistance, or other traits than either of the parents.

14. How is genetic engineering accomplished in plants? Genetic engineering is the process of altering an organisms by deliberately changing the DNA. Genetic engineering is the process of altering an organisms by deliberately changing the DNA. Transgenic plants is the plants into which genes from another organism have been incorporated. Transgenic plants is the plants into which genes from another organism have been incorporated.

15. To genetically modify an entire plant, the initial transfer of genetic information occurs at the single cell level. To genetically modify an entire plant, the initial transfer of genetic information occurs at the single cell level. Aided by the fact that plants are totipotent. Aided by the fact that plants are totipotent. Totipotent means that an organism has the ability to grow from a single cell. Totipotent means that an organism has the ability to grow from a single cell.

16. There are two common practices of moving genetic information Agrobacterium tumefacians is a bacterium that causes a disease known as crown gall in plants. Agrobacterium tumefacians is a bacterium that causes a disease known as crown gall in plants. Bacterium infects the plant by transferring its genetic material into the plant cell Bacterium infects the plant by transferring its genetic material into the plant cell Accomplished by moving the plasmid DNA of the bacteria into an injured site on the plant Accomplished by moving the plasmid DNA of the bacteria into an injured site on the plant

17. Ballistic gene transfer is the use of tiny DNA-coated projectiles as carriers (used when agrobacterium tumefacians are not effective) Ballistic gene transfer is the use of tiny DNA-coated projectiles as carriers (used when agrobacterium tumefacians are not effective) Used to transport DNA through the walls of intended recipient cells Used to transport DNA through the walls of intended recipient cells Microprojectiles are those projectiles and are tiny gold particles Microprojectiles are those projectiles and are tiny gold particles

18. Success rate is from less than 1% to about 10% Success rate is from less than 1% to about 10% Accomplished with a gene gun or microprojectile unit Accomplished with a gene gun or microprojectile unit

19. Review What is plant breeding? What is plant breeding? What are selection and hybridization? What are selection and hybridization? How is genetic engineering accomplished in plants? How is genetic engineering accomplished in plants?

20. The End!