1. Understanding the Microscope Honors Biology

2. Compound Microscope Contains a combination of two lenses

3. Eyepiece or ocular lens Lens closest to the eye

4. Objective Lens Lens closest to the specimen or object

5. How to figure out magnification Eyepiece X objective = Magnification 10x X

6. Nosepiece Objectives fastened to this revolving structure

7. Stage Where you place your slide

8. Stage clips Hold slide in place

9. Body Tube Light travels through here

10. Light Source Provides light

11. Diaphragm Regulates the light reaching the objective lens

12. Base Supports the microscope

13. Arm Holds the body tube, acts as a handle

14. Coarse focusing knob Used to focus under low power only

15. Fine focusing knob Used to focus under high power only

16. A microscope reverses and inverts the image of an object seen under it e e Before viewing under the microscope While viewing under the microscope

17. Things moving under a microscope are actually moving in the opposite direction

19. Stereomicroscope Binocular microscope Object is seen in 3-D Only used to see large objects Does not reverse or invert images

20. Transmission Electron Microscope Uses a beam of electrons instead of light rays 200,000x magnification tissues have to be sliced really thin, dry and in a vacuum chamber can’t be used with living material

23. Scanning Electron Microscope provides images with 3-D quality can’t be used with living material

28. Micromanipulator Used to dissect cells, can remove nuclei Knowledge of cells has been increased by manipulation and dissection of cells

30. High – Speed Centrifuge Spin at high speeds Cell components sort out at different layers according to density

31. Microscopic Measurements Use the unit called the micrometer 1/1000 of a meter

32. How to convert millimeters to micrometers Multiply by one thousand…or Move the decimal point 3 places to the right